US4157383A
Expired / Lapsed
US
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Tanned thrombocytes
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1976-10-13
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Granted: 1979-06-05
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Expires: 1996-10-13
Abstract
Abstract The invention provides tanned thrombocytes, a process for preparing them and a diagnostic agent containing them to make evident antibodies of thrombocytes.
Inventors
- 👤 Hans-Harald Sedlacek
- 👤 Roloff Johannsen
- 👤 Fritz Seiler
- 👤 Hermann Karges
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:47
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554; A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/19; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
54 words
In a test method for the detection of antigens or antibodies of thrombocytes by the formation of immune complexes using thrombocytes as a diagnostic reagent, the improvement wherein said thrombocytes are tanned by suspending freshly-obtained thrombocytes in an isotonic, approximately neutral, aqueous solution containing a protective colloid and treating them with a tanning agent.
Claim 2
Dependent Claim
54 words
In a test method for the detection of antigens or antibodies of thrombocytes by the formation of immune complexes using thrombocytes as a diagnostic reagent, the improvement wherein said thrombocytes are tanned by suspending freshly-obtained thrombocytes in an isotonic, approximately neutral, aqueous solution containing a protective colloid and treating them with a tanning agent.
Claim 3
Dependent Claim
15 words
The method as in claim 1 wherein said tanning agent is sulfosalicylic acid or glutardialdehyde.
Claim 4
Dependent Claim
14 words
The method as in claim 1 wherein said protective colloid is a lyophilic colloid.
Claim 5
Dependent Claim
17 words
The method as in claim 1 wherein said aqueous solution additionally contains a chelating agent for metals.
Claim 6
Independent Claim
63 words
The method of tanning thrombocytes which comprises suspending freshly-obtained thrombocytes in an isotonic, approximately neutral, aqueous solution containing a protective colloid and treating them with sulfosalicylic acid at a concentration from 0.002 to 4 percent, at a pH of 6 to 8, at a temperature of 0° C. to 37° C., and for a time from a few minutes up to several hours.
Claim 7
Independent Claim
63 words
The method of tanning thrombocytes which comprises suspending freshly-obtained thrombocytes in an isotonic, approximately neutral, aqueous solution containing a protective colloid and treating them with sulfosalicylic acid at a concentration from 0.002 to 4 percent, at a pH of 6 to 8, at a temperature of 0° C. to 37° C., and for a time from a few minutes up to several hours.
Claim 8
Dependent Claim
14 words
The method as in claim 5 wherein said protective colloid is a lyophilic colloid.
Claim 9
Dependent Claim
27 words
The method as in claim 6 wherein said lyophilic colloid is polyvinyl pyrrolidone and is present in said solution at a concentration from 0.01 to 5 percent.
References Cited (Backward Citations)
US3553310A
Cited
US3753357A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-06-04 | 1982-12-04 | 1983-06-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-06-04 | 1986-12-04 | 1987-06-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-06-04 | 1990-12-04 | 1991-06-05 | $7700.00 | Window Closed / Lapsed |