US4153668A
Expired / Lapsed
US
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Multi-zone analytical element and method using same
Assignee: Eastman Kodak Co
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Filing: 1978-01-03
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Granted: 1979-05-08
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Expires: 1998-01-03
Abstract
Abstract A multi-zone analytical element for the analysis of a proteinaceous or protein-bound substance contained in an aqueous liquid and a method of using the same are disclosed. The multi-zone element contains a spreading zone and a reagent zone in fluid contact. The reagent zone of the element contains an interactive composition. Incorporated in the spreading zone of the element is an immobilized polymeric material containing charge-bearing functional groups that, under conditions of use of the element, exhibit an electric charge opposite to that of said proteinaceous substance or the protein portion of said protein-bound substance.
Inventors
- 👤 Doyle E. Hill
- 👤 Karl J. Sanford
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/526; G01N33/68; G01N33/6803; G01N33/6827; G01N33/683; G01N33/6833; G01N33/6839; G01N33/92
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
133 words
In a multi-zone analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous liquid, said element comprising (1) a spreading zone to receive and distribute therewithin an applied sample of said aqueous liquid, and (2) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to form a detectable species within said element, the improvement wherein said spreading zone comprises an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
Claim 2
Dependent Claim
133 words
In a multi-zone analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous liquid, said element comprising (1) a spreading zone to receive and distribute therewithin an applied sample of said aqueous liquid, and (2) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to form a detectable species within said element, the improvement wherein said spreading zone comprises an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
Claim 3
Dependent Claim
22 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material has a molecular weight in excess of about 10,000.
Claim 4
Dependent Claim
17 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material is an anionic polymer.
Claim 5
Dependent Claim
30 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material contains a charge-bearing functional group selected from the group consisting of carboxy, sulfonic, sulfonate, and phosphonic groups.
Claim 6
Dependent Claim
16 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material is carboxymethyl cellulose.
Claim 7
Dependent Claim
31 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material contains a charge-bearing functional group which exhibits a positive charge at a pH of at least about 8.0.
Claim 8
Dependent Claim
17 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material contains an amino group.
Claim 9
Dependent Claim
17 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material is a cationic polymer.
Claim 10
Dependent Claim
24 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material is a cationic polymer containing an ammonium or a phosphonium group.
Claim 11
Dependent Claim
18 words
A multi-zone analytical element as defined in claim 1 wherein said polymeric material is poly(vinyl benzyltrimethyl ammonium chloride).
Claim 12
Dependent Claim
127 words
An integral multilayer analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous fluid, said element comprising a radiation-transmissive support bearing a reagent layer and, superposed thereover, an isotropically porous spreading layer, (a) said reagent layer being in fluid contact with said spreading layer under conditions of use of said element and comprising a composition interactive with said analyte to provide a radiometrically detectable change within said element, and (b) said spreading layer comprising an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
Claim 13
Dependent Claim
127 words
An integral multilayer analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous fluid, said element comprising a radiation-transmissive support bearing a reagent layer and, superposed thereover, an isotropically porous spreading layer, (a) said reagent layer being in fluid contact with said spreading layer under conditions of use of said element and comprising a composition interactive with said analyte to provide a radiometrically detectable change within said element, and (b) said spreading layer comprising an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
Claim 14
Independent Claim
150 words
A method for the analysis of a proteinaceous or protein-bound analyte in an aqueous liquid sample, said method comprising (1) contacting said aqueous liquid sample together with a multi-zone analytical element having (a) a spreading zone to receive and distribute therewithin said sample and (b) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to provide a detectable change in said element, said spreading zone containing an immobile polymeric material which (i) is substantially non-interfering with respect to said interactive composition and (ii) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte; and (2) detecting, after a predetermined time, the detectable change produced in said element.
Claim 15
Independent Claim
150 words
A method for the analysis of a proteinaceous or protein-bound analyte in an aqueous liquid sample, said method comprising (1) contacting said aqueous liquid sample together with a multi-zone analytical element having (a) a spreading zone to receive and distribute therewithin said sample and (b) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to provide a detectable change in said element, said spreading zone containing an immobile polymeric material which (i) is substantially non-interfering with respect to said interactive composition and (ii) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte; and (2) detecting, after a predetermined time, the detectable change produced in said element.
Claim 16
Dependent Claim
64 words
An integral multilayer analytical element as defined in claim 11 wherein said element is for the detection of total protein, said element comprising in the reagent layer thereof a composition interactive with total protein to provide said detectable change, and said element comprising as said polymeric material contained in the spreading layer thereof a cationic polymer or a polymer having amino groups appended thereto.
Claim 17
Dependent Claim
54 words
An integral multilayer analytical element as defined in claim 11 wherein said element is for the detection of albumin, said element comprising in the reagent layer thereof a composition interactive with albumin to provide said detectable change, and said element comprising an anionic polymer as said polymeric material contained in the spreading layer thereof.
Claim 18
Dependent Claim
54 words
An integral multilayer analytical element as defined in claim 11 wherein said element is for the detection of triglyceride, said element comprising in the reagent layer thereof a composition interactive with triglyceride to provide said detectable change, and said element comprising an anionic polymer as said polymeric material contained in the spreading layer thereof.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-05-07 | 1982-11-06 | 1983-05-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-05-07 | 1986-11-06 | 1987-05-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-05-07 | 1990-11-06 | 1991-05-08 | $7700.00 | Window Closed / Lapsed |