Patent US5938672A successfully refreshed from Google Patents.
US4152410A
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Diagnosis reagent for neoplasm and method for diagnosis of neoplasm
Assignee: Nippon Kayaku Co Ltd
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Filing: 1976-09-01
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Granted: 1979-05-01
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Expires: 1996-09-01
Abstract
Abstract NEA (Neoplasm embryonic antigen) and NEA antibody; diagnosis reagents for neoplasm comprising a substance selected from the group consisting of NEA antibody, NEA or NEA antibody labelled with a radioactive isotope, a combined product of NEA or NEA antibody with a particulate substance, a gel plate containing NEA antibody, and a carbamylated NEA antibody; methods for the diagnosis of neoplasm; processes for the production of NEA antibody; and methods for the purification of NEA and NEA antibody. The reagents are very useful for the diagnosis of neoplasms.
Inventors
- 👤 Masaru Ishii
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 02:39
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4748; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/5757; A; A61; A61K; A61K39/00
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
37 words
Substantially pure neoplasm embryonic antigen, characterized by a molecular weight of about 100,000±20,000, a photo-absorption coefficient of E 1 cm 0 .1% 280=0.936, an isoelectric point of pH 9.1-9.4, and movement in the γ-globulin region by immunoelectrophoresis.
Claim 2
Dependent Claim
37 words
Substantially pure neoplasm embryonic antigen, characterized by a molecular weight of about 100,000±20,000, a photo-absorption coefficient of E 1 cm 0 .1% 280=0.936, an isoelectric point of pH 9.1-9.4, and movement in the γ-globulin region by immunoelectrophoresis.
Claim 3
Dependent Claim
15 words
Diagnosis reagent for neoplasm, comprising an antibody of the neoplasm embryonic antigen of claim 1.
Claim 4
Dependent Claim
47 words
Diagnosis reagent for neoplasm comprising a gel plate which is prepared by dissolving an antibody of the neoplasm embryonic antigen of claim 1 in a hot solution of supporting medium selected from agar, agarose, starch, and polyacrylamide, and cooling the resulting solution to form a solid plate.
Claim 5
Dependent Claim
15 words
Diagnosis reagent for neoplasm as claim in claim 3, wherein the supporting medium is agarose.
Claim 6
Dependent Claim
20 words
Diagnosis reagent for neoplasm, comprising an antibody of the neoplasm embryonic antigen of claim 1, which antibody has been carbamylated.
Claim 7
Dependent Claim
27 words
Diagnosis reagent for neoplasm, comprising the neoplasm embryonic antigen of claim 1 labelled with a radioactive isotope or neoplasm embryonic antigen antibody labelled with a radioactive isotope.
Claim 8
Dependent Claim
16 words
Diagnosis reagent for neoplasm as claimed in claim 6, wherein the radioactive isotope is 125 I.
Claim 9
Dependent Claim
27 words
Diagnosis reagent for neoplasm, comprising the neoplasm embryonic antigen of claim 1, bound to a particulate substance or neoplasm embryonic antigen antibody bound to a particulate substance.
Claim 10
Dependent Claim
36 words
Diagnosis reagent for neoplasm as claimed in claim 8, wherein the particulate substance is selected from the group consisting of blood cells of mammals and birds, polystyrene latex, polyester latex, vinyl chloride, bentonite, and glass beads.
Claim 11
Dependent Claim
17 words
Diagnosis reagent for neoplasm as claimed in claim 8, wherein the particulate substance is erythrocytes of sheep.
Claim 12
Dependent Claim
20 words
Diagnosis reagent for neoplasm as claimed in claim 8, wherein the particulate substance is blood cells of mammals or birds.
Claim 13
Dependent Claim
36 words
Diagnosis reagent for neoplasm as claimed in claim 8, comprising a product which is prepared by coating glutaraldehyde-tannic acid treated blood cells of mammals or birds with the neoplasm embryonic antigen or neoplasm embryonic antigen antibody.
Claim 14
Dependent Claim
17 words
Diagnosis reagent for neoplasm as claimed in claim 12, wherein the blood cells are erythrocytes of sheep.
Claim 15
Dependent Claim
83 words
Method for the diagnosis of human neoplasm by detecting the neoplasm embryonic antigen of claim 1, which comprises (a) mixing an antibody of the neoplasm embryonic antigen of claim 1, the neoplasm embryonic antigen of claim 1 labelled with a radioactive isotope, and test serum from the human, (b) separating the radioactive neoplasm embryonic antigen bound to the neoplasm embryonic antigen antibody from the radioactive neoplasm embryonic antigen in the reaction mixture, and (c) measuring the radioactivity of either of said separate fractions.
Claim 16
Dependent Claim
15 words
The method of claim 14, wherein the radioactive isotope in step (a) is 125 I.
Claim 17
Dependent Claim
42 words
The method of claim 14, wherein the separation in step (b) is carried out by adding to the reaction mixture a second antibody which is able to bind to an neoplasm embryonic antigen-neoplasm embryonic antigen antibody complex, and collecting the resulting precipitate.
Claim 18
Dependent Claim
14 words
The method of claim 16, wherein the second antibody is anti-rabbit γ-globulin goat serum.
Claim 19
Dependent Claim
48 words
Method for the diagnosis of human neoplasm by detecting the neoplasm embryonic antigen of claim 1, which comprises mixing test serum from the human with an antibody of the neoplasm embryonic antigen of claim 1 bound to a particulate substance, and observing whether agglutination occurs in the mixture.
Claim 20
Dependent Claim
16 words
The method of claim 18, wherein the particulate substance is blood cells of mammals or birds.
Claim 21
Dependent Claim
12 words
The method of claim 18, wherein the particulate substance is sheep erythrocytes.
Claim 22
Dependent Claim
58 words
Method for the diagnosis of human neoplasm by detecting the neoplasm embryonic antigen of claim 1, which comprises injecting test serum from the human into a well in a gel plate of 1-2 mm in thickness containing an antibody of the neoplasm embryonic antigen of claim 1, and observing whether a precipation ring is formed on the plate.
Claim 23
Dependent Claim
14 words
The method of claim 21, wherein the gel plate is an agarose gel plate.
Claim 24
Dependent Claim
94 words
Method for the diagnosis of human neoplasm by detecting the neoplasm embryonic antigen of claim 1, which comprises (a) making a pair of wells in an agar plate of 1-2 mm in thickness, (b) injecting a carbamylated antibody of the neoplasm embryonic antigen of claim 1 into the well on the cathode side of the plate and injecting test serum from the human into the well on the anode side of the plate, (c) transmitting an electric current to the agar plate, and (d) observing whether a precipitation line is formed on the plate.
Claim 25
Dependent Claim
37 words
Process for the production of an antibody of the neoplasm embryonic antigen of claim 1, which comprises immunizing animals other than humans with the neoplasm embryonic antigen, and recovering antiserum containing the antibody from the immunized animals.
Claim 26
Dependent Claim
11 words
The process of claim 24, wherein the animal is a rabbit.
Claim 27
Dependent Claim
23 words
The process of claim 24, wherein the immunization is carried out with an emulsion of the neoplasm embryonic antigen and Freund complete adjuvant.
Claim 28
Dependent Claim
60 words
Method for obtaining the neoplasm embryonic antigen of claim 1, characterized by subjecting a material containing the neoplasm embryonic antigen of claim 1 to chromatography on a supporting material bound to an neoplasm embryonic antigen antibody to form an neoplasm embryonic antigen-neoplasm embryonic antigen antibody complex on the supporting material, and dissociating the complex to recover the neoplasm embryonic antigen.
Claim 29
Dependent Claim
59 words
Method for obtaining an antibody of the neoplasm embryonic antigen of claim 1, characterized by subjecting a material containing neoplasm embryonic antigen antibody to chromatography on a supporting material bound to neoplasm embryonic antigen to form an neoplasm embryonic antigen-neoplasm embryonic antigen antibody complex on the supporting material, and dissociating the complex to recover the neoplasm embryonic antigen antibody.
Claim 30
Dependent Claim
50 words
Purification method for obtaining the neoplasm embryonic antigen of claim 1, characterized by subjecting a material containing the neoplasm embryonic antigen of claim 1 to chromatography on a basic anion exchange resin, thereby absorbing impurities on the resin, and recovering the eluate containing the neoplasm embryonic antigen of claim 1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-30 | 1982-10-30 | 1983-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-30 | 1986-10-30 | 1987-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-30 | 1990-10-30 | 1991-05-01 | $7700.00 | Window Closed / Lapsed |