US4101381A
Expired / Lapsed
US
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Method and reagent for the determination of substances forming hydrogen peroxide
Assignee: Roche Diagnostics GmbH
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Filing: 1976-12-27
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Granted: 1978-07-18
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Expires: 1996-12-27
Abstract
Abstract In a conventional method for the quantitative determination of substances, particularly in serum, which, by action of an oxidase thereon, combine with oxygen to form hydrogen peroxide and wherein the hydrogen peroxide formed is converted, in the presence of methanol and catalase, to formaldehyde, the formaldehyde is reacted with hydrazone in the presence of ferric chloride, and the dye thus formed is determined photometrically, the improvement comprising using as the hydrazone, 3-methyl-2-sulfonylbenzothiazolone-hydrazone; use of this hydrazone results in the formation of a very easily soluble dye and is suited for use in automatic analyzers.
Inventors
- 👤 Sigmar Klose
- 👤 Ulfert Deneke
- 👤 Erich Haid
- 👤 Gunter Weimann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:21
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/32
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
65 words
Method for the quantitative determination of a substance which forms H 2 O 2 with oxygen under the action of an oxidase, which comprises treating the H 2 O 2 formed with methanol and catalase to yield formaldehyde, reacting the formaldehyde with 3-methyl-2-(sulfonyl)-benzothiazolone-hydrazone in the presence of an oxidizer, and photometrically determining the dye formed thereby as a measure of the said substance initially present.
Claim 2
Dependent Claim
65 words
Method for the quantitative determination of a substance which forms H 2 O 2 with oxygen under the action of an oxidase, which comprises treating the H 2 O 2 formed with methanol and catalase to yield formaldehyde, reacting the formaldehyde with 3-methyl-2-(sulfonyl)-benzothiazolone-hydrazone in the presence of an oxidizer, and photometrically determining the dye formed thereby as a measure of the said substance initially present.
Claim 3
Dependent Claim
17 words
Method as claimed in claim 1 wherein uric acid is determined and the said oxidase is uricase.
Claim 4
Dependent Claim
17 words
Method as claimed in claim 1 wherein glucose is determined and the said oxidase is glucose oxidase.
Claim 5
Dependent Claim
16 words
Method as claimed in claim 1 wherein cholesterol is determined and said oxidase is cholesterol oxidase.
Claim 6
Dependent Claim
13 words
Method as claimed in claim 1 wherein the oxidizer is potassium cyanoferrate (III).
Claim 7
Dependent Claim
13 words
Method as claimed in claim 1 wherein the oxidizer is FeCl 3 .
Claim 8
Dependent Claim
19 words
Method as claimed in claim 1 wherein the oxidizer is (NH 4 ) 4 -Ce(SO 4 ) 4 .
Claim 9
Dependent Claim
25 words
Method as claimed in claim 1 wherein said oxidizer is used in the form of a 0.2 to 5% solution thereof in a dilute acid.
Claim 10
Dependent Claim
21 words
Method as claimed in claim 1 wherein the said dye is formed at a pH value of between 0.5 and 3.
Claim 11
Dependent Claim
39 words
Reagent for the quantitative determination of substances in the serum which form H 2 O 2 with oxygen under the action of oxidase, containing an oxidase, catalase, methanol, buffer, an oxidizer and acid, characterized by a content of 3-methyl-2-(sulfonyl)-benzothiazolonehydrazone.
Claim 12
Dependent Claim
39 words
Reagent for the quantitative determination of substances in the serum which form H 2 O 2 with oxygen under the action of oxidase, containing an oxidase, catalase, methanol, buffer, an oxidizer and acid, characterized by a content of 3-methyl-2-(sulfonyl)-benzothiazolonehydrazone.
Claim 13
Dependent Claim
53 words
Reagent for the determination of uric acid, characterized in that it contains more than 0.1 U/ml of uricase, more than 800 U/ml of catalase, 0.02 - 0.1 M buffer pH 7 - 9, 2-8 vol. % of methanol, 0.01-0.1% of a non-ionic surface active agent, and separately therefrom 0.03 - 0.5% of 3-methyl-2-(sulfonyl)-benzothiazolonehydrazone,
Claim 14
Dependent Claim
53 words
Reagent for the determination of uric acid, characterized in that it contains more than 0.1 U/ml of uricase, more than 800 U/ml of catalase, 0.02 - 0.1 M buffer pH 7 - 9, 2-8 vol. % of methanol, 0.01-0.1% of a non-ionic surface active agent, and separately therefrom 0.03 - 0.5% of 3-methyl-2-(sulfonyl)-benzothiazolonehydrazone,
Claim 15
Dependent Claim
11 words
3 - 3% of potassium cyanoferrate III in dilute sulfuric acid.
Claim 16
Dependent Claim
11 words
3 - 3% of potassium cyanoferrate III in dilute sulfuric acid.
Claim 17
Dependent Claim
72 words
Reagent as claimd in claim 11 characterized in that it contains more than 0.05 U/ml of cholesteroloxidase, more than 0.05 U/ml of cholesterolesterase, more than 800 U/ml of catalase, 0.2 - 1.0 M of buffer pH 6.0 - 8.5, 2 - 8 vol. % of methanol, 0.1 - 0.8% of non-ionic surface active agent and separately therefrom 0.03 - ≡% of 3-methyl-2-(sulfonyl)-benzothiazolonehydrazone, 0.5 - 3.0% of FeCl 3 in dilute sulfuric acid.
Claim 18
Dependent Claim
20 words
Reagent as claimed in claim 11, characterized in that it contains, as the buffer, borate buffer pH 8.0 to 8.6.
Claim 19
Dependent Claim
20 words
Reagent as claimed in claim 12, characterized in that, as the buffer, it contains phosphate buffer pH 6.5 to 7.5.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-07-17 | 1982-01-16 | 1982-07-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-07-17 | 1986-01-16 | 1986-07-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-07-17 | 1990-01-16 | 1990-07-18 | $7700.00 | Window Closed / Lapsed |