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US4094646A Expired / Lapsed US ⏱️ 0.0 years remaining

Rapid method of assaying collagen in meat and meat products

Assignee: Baltimore Spice Co
•
Filing: 1977-06-02
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Granted: 1978-06-13
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Expires: 1997-06-02
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Abstract

Abstract A rapid method for assaying collagen in meat and meat products. A sample of the material being tested is subjected to a controlled, incomplete hydrolysis of the amino acids with an acidified solution of stannous chloride in such a way that all of the tryptophan is completely destroyed but only a portion of the hydroxyproline is hydrolyzed. The percent collagen is correlated with the percent hydroxyproline, colorimetrically determined.

Inventors

  • 👤 David L. Stern
  • 👤 Daniel B. Samchuck

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 19:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/02; G01N33/12; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555

Claims Analysis

Total Claims: 4
Claim 1
Independent Claim 61 words
A rapid method for assaying collagen in a simple of meat or meat products comprising hydrolyzing the amino acids in said sample under conditions such that all of the tryptophan therein is destroyed and only a portion of the hydroxyproline therein is hydrolyzed and then determining the percent collagen by colorimetrically reading the present hydroxyproline which has been hydrolyzed and extracted.
Claim 2
Dependent Claim 61 words
A rapid method for assaying collagen in a simple of meat or meat products comprising hydrolyzing the amino acids in said sample under conditions such that all of the tryptophan therein is destroyed and only a portion of the hydroxyproline therein is hydrolyzed and then determining the percent collagen by colorimetrically reading the present hydroxyproline which has been hydrolyzed and extracted.
Claim 3
Dependent Claim 20 words
The method of claim 1 wherein the hydrolysis is effected in an acidified solution of tin chloride (SnCl 2 ).
Claim 4
Dependent Claim 28 words
The method of claim 2 wherein the hydrolysis is effected by boiling said sample for between 20 minutes and 90 minutes in said acidified solution of stannous chloride.

References Cited (Backward Citations)

US3936271A Cited
US3980434A Cited
US4009390A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1981-06-12 1981-12-12 1982-06-13 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1985-06-12 1985-12-12 1986-06-13 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1989-06-12 1989-12-12 1990-06-13 $7700.00 Window Closed / Lapsed

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