US4082613A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of insulin by genetically transformed fungal cells
Assignee: University of Minnesota System
•
Filing: 1976-04-23
•
Granted: 1978-04-04
•
Expires: 1996-04-23
Abstract
Abstract A process for the production of insulin using fungal cells. Human insulin producing cells are extracted to obtain the functional genome (the genetic material which determines the capacity of the cells to produce insulin) which is used to transform fungal cells, making them capable of producing insulin. The insulin has been extracted and identified by radioimmune assay and by bioassay. The same process can also be applied to produce animal insulins using specific specie transforming functional insulinogenic genomes. A process for serial secondary culture of insulin producing cells is disclosed. The significance of the invention is related to the great need to produce human insulin in large amounts. Human insulin has distinct biological and clinical advantages when compared with commercially available animal insulins now used for the treatment of diabetes mellitus in man.
Inventors
- 👤 Mandayam J. Thirumalachar
- 👤 Mandayam J. Narasimhan
- 👤 John A. Anderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 07:59
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
111 words
A process for the production of insulin by genetically transformed fungal cells which comprises: A. selectively growing insulin producing beta-epithelioid cells from a pancreas and serially sub-culturing the cells under aerated cell growth conditions in a nutrient amino acid-rich medium, B. inoculating the rapid growth fungus Pseudosaccharomycete TC-1176 with genomic material extracted from the sub-culture cells and incubating in the presence of an anti-fungal membrane permeability agent to incorporate the functional genome into the fungal cell structure, C. incubating the resultant bio-transformed fungal cells in a carbohydrate-nitrogen-rich medium under cell growth conditions, and D. separating the fungal cells from the media and extracting the insulin from the cells and supernatant media.
Claim 2
Dependent Claim
111 words
A process for the production of insulin by genetically transformed fungal cells which comprises: A. selectively growing insulin producing beta-epithelioid cells from a pancreas and serially sub-culturing the cells under aerated cell growth conditions in a nutrient amino acid-rich medium, B. inoculating the rapid growth fungus Pseudosaccharomycete TC-1176 with genomic material extracted from the sub-culture cells and incubating in the presence of an anti-fungal membrane permeability agent to incorporate the functional genome into the fungal cell structure, C. incubating the resultant bio-transformed fungal cells in a carbohydrate-nitrogen-rich medium under cell growth conditions, and D. separating the fungal cells from the media and extracting the insulin from the cells and supernatant media.
Claim 3
Dependent Claim
15 words
A process according to claim 1 further characterized in that said pancreas is human pancreas.
Claim 4
Dependent Claim
220 words
A process according to claim 1 for the production of insulin by genetically transformed fungal cells which comprises: A. selectively obtaining insulin producing beta epithelioid cells from a pancreas, B. dispersing said cells in a nutrient amino acid-rich medium composition in an open aeration incubation vessel maintained at about 32° to 39° C, C. initially maintaining said cells under aerated cell growth conditions for several days, D. serially sub-culturing the resultant initial culture every several days and reconstituting the original volume with additional medium, E. maintaining the sub-cultures under aerated cell growth conditions for a further several days until the desired optimum cell growth level is reached, F. separating the cells from the media and extracting genomic material from the cells, G. dispersing said genomic material (functional genome) in a further nutrient amino acid-rich medium in an incubation vessel, H. inoculating said dispersion with Pseudosaccharomycete TC-1176, I. incubating the culture under cell growth conditions in the presence of an anti-fungal membrane permeability agent and mitogen to incorporate the functional genome into the fungal cell structure, J. transferring the resultant transformed fungal cells to a carbohydrate-nitrogen-rich medium, K. maintaining the culture under cell growth conditions until the desired optimum fungal growth level is reached, L. separating the cells from the media and extracting insulin from the cells and from the media.
Claim 5
Dependent Claim
20 words
A process according to claim 3 further characterized in that said pancreas is human and said insulin is human insulin.
Claim 6
Dependent Claim
36 words
A process according to claim 3 further characterized in that the amino acid-rich medium is Medium 199 with Earle's base supplemented by the addition of minor amounts of liver extract, hydrocortisone and anti-bacterial and anti-fungal antibiotics.
Claim 7
Dependent Claim
36 words
A process according to claim 5 further characterized in that said medium composition includes about 5 to 20 ml of liver extract and about 5 to 20 mg of hydrocortisone sulfate per liter of Medium 199.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-04-03 | 1981-10-03 | 1982-04-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-04-03 | 1985-10-03 | 1986-04-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-04-03 | 1989-10-03 | 1990-04-04 | $7700.00 | Window Closed / Lapsed |