Blood fractionation process using block copolymers of ethylene oxide and polyoxypropylene

Claim 2

A process for fractionating blood serum and plasma comprising selective precipitation of said material by admixing with a precipitating amount from about 1% to about 35% on a weight per volume basis of a compound of the formula ##STR2## wherein a and c are integers such that the hydrophile portion represented by (CH 2 CH 2 O) constitutes at least about 50% of the molecule and b is an integer such that the hydrophobic portion represented by ##STR3## has a molecular weight of at least about 950.

Claim 3

The process of claim 1 in which the block copolymer contains about 80% of polyoxyethylene hydrophilic units in the molecule and the polyoxypropylene hydrophobic base has a molecular weight between about 950 and 1750.

Claim 4

The process of claim 1 in which blood coagulation factors are separated from serum and plasma by employing a concentration of the block copolymer of from about 1% to about 20%.

Claim 5

The process of claim 3 in which a concentrate of antihemophilic factor is obtained from cryoprecipitated plasma by admixing said cryoprecipitate in aqueous solution with about 3 to 5% by weight of the block copolymer, separating the resulting precipitate therefrom, admixing the retained supernatant with additional said block copolymer to a final concentration of about 10% and retaining the resulting precipitate as the desired concentrate.

Claim 6

The process of claim 4 in which the block copolymer contains about 80% of polyoxyethylene hydrophilic units in the molecule and the polyoxypropylene hydrophobic base has a molecular weight between about 950 to 1750.

Claim 7

The process of claim 3 in which a prothrombin complex is obtained from Cohn Fraction IV-1 by admixing said Fraction in aqueous solution with from about 0.5% to 2% tribasic calcium phosphate, suspending with resulting precipitate in aqueous solution of about 0.1 molar trisodium citrate, separating the remaining precipitate therefrom, admixing the retained supernatant with about 5% of the block copolymer, separating the resulting precipitate therefrom and admixing the retained supernatant with additional said block copolymer to a final concentration of about 20% and retaining the resulting precipitate as the desired prothrombin complex.

Claim 8

The process of claim 6 in which the block copolymer contains about 80% of polyoxyethylene hydrophilic units in the molecule and the polyoxypropylene hydrophobic base has a molecular weight between 950 to 1750.