US4070247A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic media
Assignee: Indiana University Foundation Inc
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Filing: 1977-03-30
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Granted: 1978-01-24
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Expires: 1997-03-30
Abstract
Abstract Improved diagnostic media for isolating, identifying, and classifying Enterobacteriaceae comprise an effective amount of isopropyl-beta-D-thiogalactopyranoside in order to reduce the incidence of false lactose negative determinations and to differentiate between lac inducible and lac constitutive bacteria.
Inventors
- 👤 Stanton C. Burt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:38
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/10
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
36 words
In a diagnostic medium for culturing micro-organisms comprising at least one nutrient, the improvement comprising incorporating therein an amount of isopropyl-beta-D-Thiogalactopyranoside effective to induce the production of beta galactosidase in lactose negative strains of E. Coli.
Claim 2
Dependent Claim
36 words
In a diagnostic medium for culturing micro-organisms comprising at least one nutrient, the improvement comprising incorporating therein an amount of isopropyl-beta-D-Thiogalactopyranoside effective to induce the production of beta galactosidase in lactose negative strains of E. Coli.
Claim 3
Dependent Claim
20 words
An improvement as claimed in claim 1, wherein the isopropyl-beta-D-thiogalactopyranoside is present at a level of about 0.0024-0.01%, by weight.
Claim 4
Dependent Claim
37 words
An improved diagnostic medium for use in isolating, identifying, and classifying Enterobacteriaceae comprising at least one nutrient and an amount of isopropyl-beta-D-thiogalactopyranoside effective to induce the production of beta galatosidase in lactose negative strains of E. coli.
Claim 5
Dependent Claim
37 words
An improved diagnostic medium for use in isolating, identifying, and classifying Enterobacteriaceae comprising at least one nutrient and an amount of isopropyl-beta-D-thiogalactopyranoside effective to induce the production of beta galatosidase in lactose negative strains of E. coli.
Claim 6
Dependent Claim
21 words
A diagnostic medium, as claimed in claim 3, wherein the isopropyl-beta-D-thiogalactopyranoside is present at a level of about 0.0024-0.01% by weight.
Claim 7
Dependent Claim
12 words
A diagnostic medium, as claimed in claim 3, wherein nutrient comprises lactose.
Claim 8
Dependent Claim
12 words
An improvement, as claimed in claim 1, wherein the nutrient comprises agar.
Claim 9
Independent Claim
52 words
A method for classifying microorganisms comprising the steps of: inoculating the micro-organism onto a diagnostic medium comprising at least one nutrient and an amount of isopropyl-beta-D-thiogalactopyranoside effective to induce the production of beta galactosidase in lactose negative strains of E. coli; incubating te inoculated medium; and assaying the inoculated medium for beta-galactosidease.
Claim 10
Independent Claim
52 words
A method for classifying microorganisms comprising the steps of: inoculating the micro-organism onto a diagnostic medium comprising at least one nutrient and an amount of isopropyl-beta-D-thiogalactopyranoside effective to induce the production of beta galactosidase in lactose negative strains of E. coli; incubating te inoculated medium; and assaying the inoculated medium for beta-galactosidease.
Claim 11
Dependent Claim
23 words
A method, as claimed in claim 7, wherein the isopropyl-beta-D-thiogalactopyranoside is present in the medium at a level of about 0.0024-0.01% by weight.
Claim 12
Dependent Claim
25 words
A method, as claimed in claim 7, wherein the medium is assayed for beta-galactosidase by measuring the degree to which the incubated microorganism hydrolyzes orthonitrophenyl-beta-D-galactopyranoside.
References Cited (Backward Citations)
US3870601A
Cited
US3957584A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-23 | 1981-07-25 | 1982-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-23 | 1985-07-25 | 1986-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-23 | 1989-07-25 | 1990-01-24 | $7700.00 | Window Closed / Lapsed |