US4069105A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Lidocaine antigens and antibodies
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1977-03-03
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Granted: 1978-01-17
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Expires: 1997-03-03
Abstract
Abstract Derivatives of anesthetics involving anilides, lidocaine being illustrative, are provided, having an annular amino group. A difunctional linking group is provided, which provides a link to the annular amino group and an antigen, with the resulting conjugate being employed for the preparation of antibodies. The antibodies find particular use in competitive protein binding assays. Conjugates to enzymes are prepared which find particular use in homogeneous enzyme immunoassays.
Inventors
- 👤 Prithipal Singh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; C; C07; C07K; C07K16/00; C07K16/44; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/964; Y10S436/00; Y10S436/815; Y10S436/816; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
144 words
A composition of the formula ##STR6## wherein: X and X 1 are methyl; a and b are 0 or 1, the sum of a + b being at least 1; Y is hydrogen, methyl, or may be taken together with Z to form a 6 membered ring with the carbon and nitrogen atoms to which Y and Z are respectively attached; Z and Z 1 are the same or different and are alkyl of from 1 to 4 carbon atoms, with the proviso that Z may be taken together with Y; the amino groups bonded to the aromatic ring are separated by from 3 to 4 carbon atoms; R is a linking group; A is a poly(amino acid) of at least about 5,000 molecular weight; and n is at least 1 and not more than the number of available amino groups present in A.
Claim 2
Dependent Claim
144 words
A composition of the formula ##STR6## wherein: X and X 1 are methyl; a and b are 0 or 1, the sum of a + b being at least 1; Y is hydrogen, methyl, or may be taken together with Z to form a 6 membered ring with the carbon and nitrogen atoms to which Y and Z are respectively attached; Z and Z 1 are the same or different and are alkyl of from 1 to 4 carbon atoms, with the proviso that Z may be taken together with Y; the amino groups bonded to the aromatic ring are separated by from 3 to 4 carbon atoms; R is a linking group; A is a poly(amino acid) of at least about 5,000 molecular weight; and n is at least 1 and not more than the number of available amino groups present in A.
Claim 3
Dependent Claim
11 words
A composition according to claim 1, wherein A is an antigen.
Claim 4
Dependent Claim
11 words
A composition according to claim 1, wherein A is an enzyme.
Claim 5
Dependent Claim
23 words
A composition according to claim 1, wherein Z and Z 1 are ethyl, a and b are both 1 and Y is hydrogen.
Claim 6
Dependent Claim
45 words
A composition according to claim 1, wherein R is of the formula ##STR7## wherein: E is a bond, alkylene or alkyleneamino of from 1 to 5 carbon atoms; D and D 1 are oxygen, nitrogen or sulfur; and p and q are 0 or 1.
Claim 7
Dependent Claim
22 words
A composition according to claim 5, wherein E is alkylene p and q are 1 and D and D 1 are oxygen.
Claim 8
Dependent Claim
18 words
A composition according to claim 5, wherein p is 1, q is 0 and E is a bond.
Claim 9
Dependent Claim
58 words
A composition of the formula ##STR8## wherein: A is a poly(amino acid); n is is the range of 1 to 500; R is of the formula ##STR9## wherein: E is a bond, alkylene or alkyleneamino of from 1 to 5 carbon atoms; D and D 1 are oxygen or nitrogen; and p and q are 0 or 1.
Claim 10
Dependent Claim
58 words
A composition of the formula ##STR8## wherein: A is a poly(amino acid); n is is the range of 1 to 500; R is of the formula ##STR9## wherein: E is a bond, alkylene or alkyleneamino of from 1 to 5 carbon atoms; D and D 1 are oxygen or nitrogen; and p and q are 0 or 1.
Claim 11
Dependent Claim
11 words
A composition according to claim 8, wherein A is an antigen.
Claim 12
Dependent Claim
11 words
A composition according to claim 8, wherein A is an enzyme.
Claim 13
Dependent Claim
19 words
A composition according to claim 8, wherein D and D 1 are oxygen, and p and q are 1.
Claim 14
Dependent Claim
10 words
A composition according to claim 11, wherein E is alkylene.
Claim 15
Dependent Claim
22 words
A composition according to claim 8, wherein E is a bond, D 1 is oxygen, q is 1, and p is 0.
Claim 16
Dependent Claim
12 words
A composition of the formula ##STR10## wherein: A is a poly(amino acid).
Claim 17
Dependent Claim
12 words
A composition of the formula ##STR10## wherein: A is a poly(amino acid).
Claim 18
Dependent Claim
11 words
A composition according to claim 14, wherein A is bovine γ-globulin.
Claim 19
Dependent Claim
13 words
A composition of the formula ##STR11## wherein: A is an antigenic poly(amino acid).
Claim 20
Dependent Claim
13 words
A composition of the formula ##STR11## wherein: A is an antigenic poly(amino acid).
Claim 21
Dependent Claim
11 words
A composition according to claim 16, wherein A is bovine γ-globulin.
Claim 22
Dependent Claim
12 words
A composition according to claim 16, wherein A is bovine serum alubmin.
Claim 23
Dependent Claim
11 words
A composition of the formula ##STR12## wherein: ENZ is an enzyme.
Claim 24
Dependent Claim
11 words
A composition of the formula ##STR12## wherein: ENZ is an enzyme.
Claim 25
Dependent Claim
12 words
A composition according to claim 19, wherein said enzyme is a dehydrogenase.
Claim 26
Dependent Claim
12 words
A composition according to claim 20, wherein said dehydrogenase is glucose-6-phosphate dehydrogenase.
Claim 27
Dependent Claim
12 words
An antibody prepared in response to a conjugate according to claim 2.
Claim 28
Dependent Claim
12 words
An antibody prepared in response to a conjugate according to claim 9.
Claim 29
Dependent Claim
12 words
An antibody prepared in response to a conjugate according to claim 16.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-16 | 1981-07-18 | 1982-01-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-16 | 1985-07-18 | 1986-01-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-16 | 1989-07-18 | 1990-01-17 | $7700.00 | Window Closed / Lapsed |