US4065359A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell removing device
Assignee: Merck and Co Inc
•
Filing: 1976-07-06
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Granted: 1977-12-27
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Expires: 1996-07-06
Abstract
Abstract Apparatus for physically removing cells in a sterile manner from a disc stack on which the cells have been cultured comprises a substantially shaft-like member having at least one butressed rod-like member mounted at substantially a right angle to the axis of the rod.
Inventors
- 👤 William M. Hurni
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:54
CPC Classifications
C; C12; C12M; C12M25/00; C12M25/06; C12M27/00; C12M27/14; C12M33/00
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
126 words
Apparatus for removing cells from cell culture apparatus having a plurality of discs mounted on a central shaft, the cell removing apparatus comprising a substantially shaft-like member having mounted thereon substantially perpendicularly to the axis of the shaft-like member a plurality of spaced apart contacting members, each contacting member adapted to contact at least part of the surface of a disc, each contacting member having two arms, each arm attached separately to the shaft-like member, the arms being joined a distance removed from the shaft-like member whereby the contacting member is substantially triangular in shape having its base at about the shaft-like member and its apex a distance removed from the shaft-like member, and each contacting member being moved directly upon rotation of the shaft-like member.
Claim 2
Dependent Claim
126 words
Apparatus for removing cells from cell culture apparatus having a plurality of discs mounted on a central shaft, the cell removing apparatus comprising a substantially shaft-like member having mounted thereon substantially perpendicularly to the axis of the shaft-like member a plurality of spaced apart contacting members, each contacting member adapted to contact at least part of the surface of a disc, each contacting member having two arms, each arm attached separately to the shaft-like member, the arms being joined a distance removed from the shaft-like member whereby the contacting member is substantially triangular in shape having its base at about the shaft-like member and its apex a distance removed from the shaft-like member, and each contacting member being moved directly upon rotation of the shaft-like member.
Claim 3
Dependent Claim
28 words
Apparatus according to claim 1 wherein the portion of the shaft to which each arm is attached has two substantially flat surfaces, each surface provided with a recess.
Claim 4
Dependent Claim
21 words
Apparatus according to claim 2 wherein each arm is attached to a recess provided in one of the substantially flat surfaces.
Claim 5
Dependent Claim
17 words
Apparatus according to claim 3 wherein the recesses are at an angle with respect to one another.
Claim 6
Dependent Claim
16 words
Apparatus according to claim 4 wherein the recesses are substantially perpendicular with respect to one another.
Claim 7
Dependent Claim
17 words
Apparatus according to claim 1 wherein each arm is coated with a flexible, substantially inert, impermeable material.
Claim 8
Dependent Claim
19 words
Apparatus according to claim 1 wherein each arm is covered with a tubing of flexible, substantially inert, impermeable material.
Claim 9
Dependent Claim
11 words
Apparatus according to claim 7 wherein the tubing is polyfluorinated hydrocarbon.
Claim 10
Dependent Claim
50 words
A method of physically removing cells from a disc on which the cells have been cultured comprising the step of positioning a contacting member described in claim 1 on at least part of the surface of the disc and rotating the contacting member and the disc relative to each other.
Claim 11
Dependent Claim
99 words
In a multiplate propagator comprising a substantially cylindrical vessel having a plurality of spaced apart parallel discs on which discs cells are grown, the discs mounted on a rotatable shaft within the vessel, the improvement comprising a second shaft pivotably mounted within the cylindrical vessel, the second shaft having mounted thereon contacting means adapted to contact at least part of the surface of a disc upon pivotal movement of the second shaft whereby cells are removed from the surface of the disc, the contacting means comprising a cell contacting surface having means for laterally reinforcing the cell contacting surface.
Claim 12
Dependent Claim
99 words
In a multiplate propagator comprising a substantially cylindrical vessel having a plurality of spaced apart parallel discs on which discs cells are grown, the discs mounted on a rotatable shaft within the vessel, the improvement comprising a second shaft pivotably mounted within the cylindrical vessel, the second shaft having mounted thereon contacting means adapted to contact at least part of the surface of a disc upon pivotal movement of the second shaft whereby cells are removed from the surface of the disc, the contacting means comprising a cell contacting surface having means for laterally reinforcing the cell contacting surface.
Claim 13
Dependent Claim
16 words
A propagator according to claim 10 wherein the contacting means comprises a substantially wedge shaped member.
Claim 14
Dependent Claim
15 words
A propagator according to claim 11 wherein the wedge shaped member comprises two converging arms.
Claim 15
Dependent Claim
18 words
A propagator according to claim 12 wherein each arm is attached to a recess in the second shaft.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-26 | 1981-06-27 | 1981-12-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-26 | 1985-06-27 | 1985-12-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-26 | 1989-06-27 | 1989-12-27 | $7700.00 | Window Closed / Lapsed |