US4060456A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Glucose isomerization process
Assignee: RJ Reynolds Tobacco Co
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Filing: 1975-10-14
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Granted: 1977-11-29
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Expires: 1995-10-14
Abstract
Abstract Microbial cell material having a glucose-isomerizing enzyme associated therewith is subjected to flocculation conditions to produce a flocculated aggregate containing the cell material. Glucose syrups are brought into contact with the flocculated aggregates and a portion of the glucose is thereby converted to fructose in the presence of the glucose-isomerizing enzyme.
Inventors
- 👤 Margaret E. Long
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:24
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N1/00; C12N1/02; C12P; C12P19/00; C12P19/24; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/853; Y10S435/886
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
123 words
A continuous process for isomerizing glucose to fructose in the presence of glucose isomerase-containing microbial cells wherein said cells are used in the form of an aggregate resulting from prior treatment of said cells with 0.5 to 50 percent by weight based on the wet weight of said cells of a polyelectrolyte flocculating agent followed by drying and sieving of the aggregate to produce dried aggregate particles that are approximately 10 to 30 mesh in size, said continuous process comprising passing a glucose solution through a bed of said aggregate at a temperature of 50° to 90° C. and a pH of 6 to 10 to effect isomerization of a portion of the glucose and recovering an effluent syrup containing glucose and fructose.
Claim 2
Dependent Claim
123 words
A continuous process for isomerizing glucose to fructose in the presence of glucose isomerase-containing microbial cells wherein said cells are used in the form of an aggregate resulting from prior treatment of said cells with 0.5 to 50 percent by weight based on the wet weight of said cells of a polyelectrolyte flocculating agent followed by drying and sieving of the aggregate to produce dried aggregate particles that are approximately 10 to 30 mesh in size, said continuous process comprising passing a glucose solution through a bed of said aggregate at a temperature of 50° to 90° C. and a pH of 6 to 10 to effect isomerization of a portion of the glucose and recovering an effluent syrup containing glucose and fructose.
Claim 3
Dependent Claim
29 words
A process according to claim 1 in which said aggregate is dried to a moisture level of less than 15 percent prior to use in the glucose isomerization process.
Claim 4
Dependent Claim
20 words
A process according to claim 2 in which the cells are derived from a microorganism belonging to the genus Arthrobacter.
Claim 5
Dependent Claim
20 words
A process according to claim 2 in which the cells are derived from a microorganism belonging to the genus Streptomyces.
Claim 6
Dependent Claim
142 words
A continuous process for converting glucose to fructose in the presence of glucose isomerase-containing microbial cells wherein said cells are used in the form of an aggregate resulting from prior flocculation of said cells with 0.5 to 50 percent by weight based on the wet weight of said cells of a polyelectrolyte flocculating agent selected from the group consisting of anionic and cationic polyelectrolytes followed by a. harvesting the flocculated cells, b. extruding the harvested flocculated cells, and c. drying the extruded flocculated cells to a moisture level of less than 15 percent, said continuous process comprising passing a glucose solution through a bed of said aggregate at a temperature of 50° to 90° C. and a pH of 6 to 10 to effect conversion of a portion of the glucose to fructose and recovering an effluent syrup containing glucose and fructose.
Claim 7
Dependent Claim
142 words
A continuous process for converting glucose to fructose in the presence of glucose isomerase-containing microbial cells wherein said cells are used in the form of an aggregate resulting from prior flocculation of said cells with 0.5 to 50 percent by weight based on the wet weight of said cells of a polyelectrolyte flocculating agent selected from the group consisting of anionic and cationic polyelectrolytes followed by a. harvesting the flocculated cells, b. extruding the harvested flocculated cells, and c. drying the extruded flocculated cells to a moisture level of less than 15 percent, said continuous process comprising passing a glucose solution through a bed of said aggregate at a temperature of 50° to 90° C. and a pH of 6 to 10 to effect conversion of a portion of the glucose to fructose and recovering an effluent syrup containing glucose and fructose.
Claim 8
Dependent Claim
18 words
A process according to claim 5 in which the aggregate contains both an anionic and a cationic polyelectrolyte.
Claim 9
Dependent Claim
28 words
A process according to claim 6 in which the aggregate is used in the form of particles that are approximately 10 to 30 mesh in size when dry.
Claim 10
Dependent Claim
21 words
A process according to claim 7 in which the microbial cells are derived from a microorganism belonging to the genus Arthrobacter.
Claim 11
Dependent Claim
21 words
A process according to claim 7 in which the microbial cells are derived from a microorganism belonging to the genus Streptomyces.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-11-28 | 1981-05-30 | 1981-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-11-28 | 1985-05-30 | 1985-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-11-28 | 1989-05-30 | 1989-11-29 | $7700.00 | Window Closed / Lapsed |