Immunity depressant medicine

Claim 2

A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps: a. Washing the ground placental tissue to remove serous substances therefrom; b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate; and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken at a pH of about between 2 and 3.5 with said buffer solution and at a temperature ranging between about 10° to 38° C; c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins; and d. Dialyzing the immuno-depressive gamma-globulins with water.

Claim 3

Process according to claim 1 wherein step b is carried out for a period of about 30 minutes.

Claim 4

Process according to claim 1 wherein the extraction is carried out at a temperature of about 37° C.

Claim 5

Process according to claim 1 wherein in step b the gamma-globulins are separated from the tissue elements by centrifugation and are present in the resulting eluate.

Claim 6

Process according to claim 1 wherein the pH of the supernatant is adjusted to essentially a neutral value, ethanol is added and that at least one dialysis is performed to concentrate said gamma-globulins.

Claim 7

Process according to claim 1 wherein said placental tissue is washed with a PBS buffer.

Claim 8

Process according to claim 5 including several successive washings.

Claim 9

Medicine comprising gamma-globulins produced by the process of claim 1 wherein said gamma-globulins are characterized in that they a. react with an animal anti-human-Ig serum b. have almost no lymphocytotoxicity; c. inhibit the lymphoblastic transformation of human lymphocytes stimulated by phytohemagglutinin and by various antigens; and d. inhibit the lymphoblastic transformation of histo-incompatible human lymphocytes in mixed culture.

Claim 10

Medicine according to claim 8 wherein said gamma-globulins are contained in a therapeutic serum for use by man.

Claim 11

Medicine according to claim 9 in the form of a dosage for humans containing about 500 mg of proteins.

Claim 12

Medicine according to claim 10 which also includes at least one other immuno-depressant agent.

Claim 13

Medicine according to claim 11 wherein said other immuno-depressant agent is human anti-lymphocyte serum.

Claim 14

The process of treating graft rejection in human beings suffering from auto-immunity comprising administrating the medicine of claim 8 in an amount of the order of 500 mg per day.

Claim 15

A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the steps of: a. Washing the ground placental tissue to remove serous substances therefrom; b. Eluting the placental tissues, freed of serous substances, with a buffer solution having an acid pH of 6.5 to a basic pH of 7.5 to form an eluate; and freeing the eluate of any tissue material and precipitate; c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins; and d. Dialyzing the immuno-depressive gamma-globulins with water.

Claim 16

A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the steps of: a. Washing the ground placental tissue to remove serous substances therefrom; b. Eluting the placental tissues, freed of serous substances, with a buffer solution having an acid pH of 6.5 to a basic pH of 7.5 to form an eluate; and freeing the eluate of any tissue material and precipitate; c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins; and d. Dialyzing the immuno-depressive gamma-globulins with water.

Claim 17

A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps: a. Washing the ground placental tissue to remove serous substances therefrom; b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate; and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken with said buffer solution having an acid pH and at a temperature ranging between about 10° to 38° C; c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins; and d. Dialyzing the immuno-depressive gamma-globulins with water.

Claim 18

A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps: a. Washing the ground placental tissue to remove serous substances therefrom; b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate; and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken with said buffer solution having an acid pH and at a temperature ranging between about 10° to 38° C; c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins; and d. Dialyzing the immuno-depressive gamma-globulins with water.

Claim 19

A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps: a. Washing the ground placental tissue to remove serous substances therefrom; b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate; and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken with said buffer solution which has a substantially neutral pH and at a temperature ranging between about 10° to 38° C; c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins; and d. Dialyzing the immuno-depressive gamma-globulins with water.

Claim 20

A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps: a. Washing the ground placental tissue to remove serous substances therefrom; b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate; and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken with said buffer solution which has a substantially neutral pH and at a temperature ranging between about 10° to 38° C; c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins; and d. Dialyzing the immuno-depressive gamma-globulins with water.