US4053284A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Continuous flow apparatus for biological testing
Assignee: Akzona Inc
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Filing: 1976-03-10
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Granted: 1977-10-11
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Expires: 1996-03-10
Abstract
Abstract A test apparatus for conducting diagnostic tests on body fluids, said apparatus having (1) a reaction vessel containing immunochemical test reagents supported as on beads and (2) a washing means cooperating with the reaction vessel, said washing means supplying washing fluid for the reaction vessel. The washing means also comprises a vacuum conduit for aspirating the washing fluid out of the reaction vessel. The apparatus is applicable to hepatitis testing.
Inventors
- 👤 Nancy Ann Posch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:38
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; B; B01; B01L; B01L13/00; B01L13/02; B01L3/00; B01L3/50; B01L3/508; B01L3/5082; G01N35/00; G01N35/10; G01N35/1095; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/811; Y10S436/82
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
254 words
Test apparatus for the performance of solid supported immunochemical reactions in diagnostic tests on samples of blood, serum, urine, and other liquids comprising: a reaction vessel having an open end and a closed end, which vessel (1) is fitted with a fluid permeable grid member above the closed end defining a reaction chamber between the grid and the closed end to form a reaction zone and (2) has one or more geometrical solids within said chamber with immunochemical test reagents supported thereon, said chamber adapted to receive a washing apparatus, and (3) is adapted to receive the washing apparatus in vacuum sealing engagement; and a washing apparatus having a longitudinal axis and adapted for insertion (a) into the reaction vessel through the open end thereof to form a vacuum seal therewith, and (b) into the reaction chamber, comprising (1) a container to receive fluid, said container adapted to communicate with a source of fluid located externally to the reaction vessel, said container being fitted with a plurality of ports in a band, each port equidistant from another for continuously ejecting fluid from the container into the reaction vessel, and for washing the reaction vessel and the geometrical solids therein, and (2) an elongated vacuum conduit passing through the container in parallel to the longitudinal axis thereof, said conduit having a first vent adapted to communicate with a vacuum source and a second vent communicating with the interior of the reaction chamber to capture and remove fluid as said fluid is released from the container.
Claim 2
Dependent Claim
254 words
Test apparatus for the performance of solid supported immunochemical reactions in diagnostic tests on samples of blood, serum, urine, and other liquids comprising: a reaction vessel having an open end and a closed end, which vessel (1) is fitted with a fluid permeable grid member above the closed end defining a reaction chamber between the grid and the closed end to form a reaction zone and (2) has one or more geometrical solids within said chamber with immunochemical test reagents supported thereon, said chamber adapted to receive a washing apparatus, and (3) is adapted to receive the washing apparatus in vacuum sealing engagement; and a washing apparatus having a longitudinal axis and adapted for insertion (a) into the reaction vessel through the open end thereof to form a vacuum seal therewith, and (b) into the reaction chamber, comprising (1) a container to receive fluid, said container adapted to communicate with a source of fluid located externally to the reaction vessel, said container being fitted with a plurality of ports in a band, each port equidistant from another for continuously ejecting fluid from the container into the reaction vessel, and for washing the reaction vessel and the geometrical solids therein, and (2) an elongated vacuum conduit passing through the container in parallel to the longitudinal axis thereof, said conduit having a first vent adapted to communicate with a vacuum source and a second vent communicating with the interior of the reaction chamber to capture and remove fluid as said fluid is released from the container.
Claim 3
Dependent Claim
18 words
A test apparatus as in claim 1 wherein the immunochemical reagents are covalently bound to the geometrical solids.
Claim 4
Dependent Claim
18 words
A test apparatus as in claim 1 wherein the immunochemical test reagents are adsorbed onto the geometrical solids.
Claim 5
Dependent Claim
15 words
A test apparatus as in claim 1 wherein the reaction vessel is constructed of plastic.
Claim 6
Dependent Claim
50 words
A test apparatus as in claim 1 wherein the fluid container and vacuum conduit are cylindrical tubes having parallel, spaced-apart sides and a common axis, the vacuum conduit being positioned within and extending through the container said container having closed first and second end portions mating with the vacuum conduit.
Claim 7
Dependent Claim
37 words
A test apparatus as in claim 1 for performing diagnostics tests on human blood, serum, or plasma to detect hepatitis B surface antigen wherein the geometrical solids support an antibody specific against said hepatitis B surface antigen.
Claim 8
Dependent Claim
20 words
A test apparatus as in claim 6 wherein the geometrical solids for the hepatitis antibody are a plurality of beads.
Claim 9
Dependent Claim
18 words
A test apparatus as in claim 6 wherein the hepatitis antibody is covalently bound to the geometrical solids.
Claim 10
Dependent Claim
17 words
A test apparatus as in claim 6 wherein the heptatis antibody is adsorbed onto the geometrical solids.
Claim 11
Dependent Claim
16 words
A test apparatus as in claim 6 wherein the reaction vessel is constructed of clear plastic.
Claim 12
Independent Claim
286 words
A method for washing samples of blood, urine, and other body fluids out of a reaction vessel containing said fluids during the performance of solid phase immunological reactions in diagnostic tests comprising the steps of: a. depositing said fluids into a reaction vessel having an open end and a closed end, which vessel (1) is fitted with a fluid permeable grid members above the closed end defining a reaction chamber between the grid and the closed end to form a reaction zone and (2) has one or more geometrical solids within said chamber with immunochemical test reagents supported thereon, said chamber adapted to receive a washing apparatus, and (3) is adapted to receive the washing apparatus in vacuum sealing engagement; b. inserting a washing apparatus into said vessel to mate in vacuum-tight engagement with said vessel, said washing apparatus having (1) a longitudinal axis and fitted with a container to receive fluid, said container in communication with a source of fluid located externally to the container, said container being fitted with a plurality of ports in a band, each port equidistant from another for continuously ejecting fluid from the container into the reaction vessel and for washing the reaction vessel and the geometrical solids therein, and (2) an elongated vacuum conduit passing through the container in parallel with the longitudinal axis of the container, said conduit having a first vent adapted to communicate with a vacuum source and a second vent communicating with the interior of the reaction chamber; and c. drawing through the vacuum conduit of the washing apparatus thereby ejecting fluid from the container into the reaction vessel and simultaneously capturing and removing said fluid through the vacuum conduit out of the reaction vessel.
Claim 13
Independent Claim
286 words
A method for washing samples of blood, urine, and other body fluids out of a reaction vessel containing said fluids during the performance of solid phase immunological reactions in diagnostic tests comprising the steps of: a. depositing said fluids into a reaction vessel having an open end and a closed end, which vessel (1) is fitted with a fluid permeable grid members above the closed end defining a reaction chamber between the grid and the closed end to form a reaction zone and (2) has one or more geometrical solids within said chamber with immunochemical test reagents supported thereon, said chamber adapted to receive a washing apparatus, and (3) is adapted to receive the washing apparatus in vacuum sealing engagement; b. inserting a washing apparatus into said vessel to mate in vacuum-tight engagement with said vessel, said washing apparatus having (1) a longitudinal axis and fitted with a container to receive fluid, said container in communication with a source of fluid located externally to the container, said container being fitted with a plurality of ports in a band, each port equidistant from another for continuously ejecting fluid from the container into the reaction vessel and for washing the reaction vessel and the geometrical solids therein, and (2) an elongated vacuum conduit passing through the container in parallel with the longitudinal axis of the container, said conduit having a first vent adapted to communicate with a vacuum source and a second vent communicating with the interior of the reaction chamber; and c. drawing through the vacuum conduit of the washing apparatus thereby ejecting fluid from the container into the reaction vessel and simultaneously capturing and removing said fluid through the vacuum conduit out of the reaction vessel.
Claim 14
Dependent Claim
30 words
A method as in claim 11 wherein the fluid tested is selected from the group consisting of blood, serum, and plasma and the test is for hepatitis B surface antigen.
Claim 15
Dependent Claim
93 words
A reaction vessel for diagnostic tests involving immmunochemical reactions with solid-supported reagents to detect antigens, antibodies and other etiological agents in samples of blood, serum, urine, and other liquids, having an open end, and a closed end, which vessel (1) is fitted with a fluid permeable grid member above the closed end defining a reaction chamber between the grid and the closed end to form a reaction zone and adapted to receive a washing apparatus and (2) has one or more geometrical solids within said chamber with test reagents supported on said solids.
Claim 16
Dependent Claim
93 words
A reaction vessel for diagnostic tests involving immmunochemical reactions with solid-supported reagents to detect antigens, antibodies and other etiological agents in samples of blood, serum, urine, and other liquids, having an open end, and a closed end, which vessel (1) is fitted with a fluid permeable grid member above the closed end defining a reaction chamber between the grid and the closed end to form a reaction zone and adapted to receive a washing apparatus and (2) has one or more geometrical solids within said chamber with test reagents supported on said solids.
Claim 17
Dependent Claim
15 words
The vessel recited in claim 13 wherein the geometrical solids comprise one or more beads.
Claim 18
Dependent Claim
21 words
The vessel recited in claim 13 wherein said vessel is so adapted to receive the washing apparatus in vacuum sealing engagement.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-10-10 | 1981-04-11 | 1981-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-10-10 | 1985-04-11 | 1985-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-10-10 | 1989-04-11 | 1989-10-11 | $7700.00 | Window Closed / Lapsed |