US4018885A
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Steroid-binding globulin and process for preparing it and antibodies thereto
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1974-10-24
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Granted: 1977-04-19
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Expires: 1994-10-24
Abstract
Abstract A derivative of the steroid-binding β-globulin which can be obtained therefrom by treatment with neuraminidase.
Inventors
- 👤 Hans Bohn
- 👤 Wilhelm Winckler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:10
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4717; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/909; Y10S436/00; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S530/00; Y10S530/827; Y10S530/85; Y10S530/851
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
157 words
A method for making a modified steroid-binding globulin which comprises treating a steroid-binding β-globulin starting material having the following properties: a. a specific affinity to steroid hormones, b. a content of carbohydrates of 12.5 ± 3%, of which are 5.7 ± 1.2% hexoses, 3.9 ± 0.8% hexoseamine, calculated as N-acetyl-hexoseamine, 2.9 ± 0.9% of neuraminic acid, calculated as N-acetyl-neuraminic acid, and 0.1 ± 0.1% fucose, c. the capacity of being precipitated by specific anti-sera, d. an electrophoretic mobility corresponding to that of β 1 -globulins, e. a sedimentation constant of 4.1 ± 1.0 S, measured in a phosphate buffer of pH 6.8, f. a sedimentation constant of 1.1 ± 0.3 S, measured in a 1% sodium dodecylsulfate solution, and g. a molecular weight of 65,000 ± 5,000, with neuraminidase, at a pH within the optimum reaction range of the enzyme, until the neuraminic acid content of the steroid-binding β-globulin starting material is reduced to 0.5 ± 0.5%.
Claim 2
Independent Claim
157 words
A method for making a modified steroid-binding globulin which comprises treating a steroid-binding β-globulin starting material having the following properties: a. a specific affinity to steroid hormones, b. a content of carbohydrates of 12.5 ± 3%, of which are 5.7 ± 1.2% hexoses, 3.9 ± 0.8% hexoseamine, calculated as N-acetyl-hexoseamine, 2.9 ± 0.9% of neuraminic acid, calculated as N-acetyl-neuraminic acid, and 0.1 ± 0.1% fucose, c. the capacity of being precipitated by specific anti-sera, d. an electrophoretic mobility corresponding to that of β 1 -globulins, e. a sedimentation constant of 4.1 ± 1.0 S, measured in a phosphate buffer of pH 6.8, f. a sedimentation constant of 1.1 ± 0.3 S, measured in a 1% sodium dodecylsulfate solution, and g. a molecular weight of 65,000 ± 5,000, with neuraminidase, at a pH within the optimum reaction range of the enzyme, until the neuraminic acid content of the steroid-binding β-globulin starting material is reduced to 0.5 ± 0.5%.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein said neuraminidase is a microbial neuraminidase and said pH is from 5 to 7.
Claim 4
Dependent Claim
82 words
The method of claim 1 wherein said steroid-binding β-globulin starting material is treated with neuraminidase from Vibrio cholerae at a pH of 5.5 in a ratio of 50 to 500 units of neuraminidase per 100 mg of protein, at 0° to 20° C. and for 5 to 20 hours, a longer time period within this range being used at lower temperatures within the range specified and a shorter time period within this range being used with higher temperatures within the range specified.
Claim 5
Dependent Claim
128 words
A modified steroid-binding globulin made by the method of claim 1, said modified steroid-binding globulin having the following properties: a. a specific affinity to steroid hormones, b. a content of carbohydrates of 10.4 ± 2.6%, of which are 5.8 ± 1.2% hexoses, 4.0 ± 0.8% hexoseamine, calculated as N-acetyl-hexoseamine, 0.5 ± 0.5% neuraminic acid, calculated as N-acetyl-neuramanic acid, and 0.1 ± 0.1% fucose, c. the capacity of being precipitated by anti-sera which are directed against steroid-binding β-globulin, d. an electrophoretic mobility corresponding to that of gamma-globulin, e. a sedimentation constant of 4.1 ± 1.0 S, measured in a phosphate buffer at pH 6.8, f. a sedimentation constant of 1.1 ± 0.3 S, measured in a 1% sodium dodecylsulfate solution, and g. a molecular weight of 65,000 ± 5,000.
Claim 6
Dependent Claim
32 words
In the method for making an anti-serum specific to steroid-binding globulin by immunizing animals with said steroid-binding globulin, the improvement wherein the steroid-binding globulin is the modified steroid-binding globulin of claim 4.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-04-18 | 1980-10-18 | 1981-04-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-04-18 | 1984-10-18 | 1985-04-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-04-18 | 1988-10-18 | 1989-04-19 | $7700.00 | Window Closed / Lapsed |