Patent US1336761A successfully refreshed from Google Patents.
US4017364A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing an enzyme product having variable solubility
Assignee: Nestec SA
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Filing: 1975-06-19
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Granted: 1977-04-12
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Expires: 1995-06-19
Abstract
Abstract An enzymatically active product is prepared by reacting an enzyme, e.g. trypsin, with a polymer carrying both free aldehyde groups and free acid groups, e.g. a copolymer of acroelin and acrylic acid containing at least 10 % of free aldehyde groups based on the total of free aldehyde and acid groups. The product has a variable solubility in aqueous medium so that the enzyme bound to the polymer can be recovered for reuse by adjusting the pH to precipitate the polymer.
Inventors
- 👤 Ekkehard van Leemputten
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:23
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
55 words
A process for the preparation of an enzymatically active product having a variable solubility in an aqueous medium dependent on pH, comprising reacting an enzyme with a polymer carrying both free aldehyde groups and free acid groups, said free acid groups being present in a sufficient number to ensure the variable solubility of the product.
Claim 2
Dependent Claim
55 words
A process for the preparation of an enzymatically active product having a variable solubility in an aqueous medium dependent on pH, comprising reacting an enzyme with a polymer carrying both free aldehyde groups and free acid groups, said free acid groups being present in a sufficient number to ensure the variable solubility of the product.
Claim 3
Dependent Claim
29 words
A process according to claim 1, in which said polymer contains at least 5 % of free acid groups based on the total of free aldehyde and acid groups.
Claim 4
Dependent Claim
37 words
A process according to claim 1 in which said polymer is a copolymer of acrolein and acrylic acid containing at least 10 % of free aldehyde groups based on the total of free aldehyde and acid groups.
Claim 5
Dependent Claim
11 words
A process according to claim 2 wherein said enzyme is trypsin.
Claim 6
Dependent Claim
12 words
A process according to claim 3, in which said enzyme is trypsin.
Claim 7
Dependent Claim
35 words
A process according to claim 1, in which said enzyme is reacted with said polymer by mixing said two substances in a buffer solution to produce a product which is precipitated and collected by filtration.
Claim 8
Dependent Claim
18 words
A process according to claim 6, in which said buffer solution has a pH-value of 7 to 8.
Claim 9
Dependent Claim
12 words
A process according to claim 7, in which said enzyme is trypsin.
Claim 10
Dependent Claim
80 words
A process for the production of an enzymatically active product having a variable solubility in aqueous medium comprising reacting an enzyme with a copolymer of acrolein and acrylic containing at least 10 % of free aldehyde groups, based on the total of free aldehyde and acid groups, by mixing said enzyme and said copolymer in a buffer solution with vigorous stirring for 1 to 3 hours at ambient temperature to produce a product which is precipitated and collected by filtration.
Claim 11
Dependent Claim
80 words
A process for the production of an enzymatically active product having a variable solubility in aqueous medium comprising reacting an enzyme with a copolymer of acrolein and acrylic containing at least 10 % of free aldehyde groups, based on the total of free aldehyde and acid groups, by mixing said enzyme and said copolymer in a buffer solution with vigorous stirring for 1 to 3 hours at ambient temperature to produce a product which is precipitated and collected by filtration.
Claim 12
Dependent Claim
41 words
A process according to claim 9 in which said reaction is carried out in aqueous homogeneous phase using a buffer of about pH 8 for about 1 hour after which said pH is lowered to about 4 to precipitate said product.
Claim 13
Dependent Claim
12 words
A process according to claim 10 in which said enzyme is trypsin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-04-11 | 1980-10-11 | 1981-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-04-11 | 1984-10-11 | 1985-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-04-11 | 1988-10-11 | 1989-04-12 | $7700.00 | Window Closed / Lapsed |