US4017261A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biological diagnostic test strip and method of producing same
Assignee: Lachema
•
Filing: 1975-10-16
•
Granted: 1977-04-12
•
Expires: 1995-10-16
Abstract
Abstract Diagnostic test strips for the qualitative detection and semi-quantitative estimation of blood and hemoglobin in biological products include a reagent area comprising an acid buffer having a pH within the range of 2.5-5.0, a chromogen, a wetting agent, an agent capable of enhancing the peroxidase activity of hemoglobin, an organic hydroperoxide in the form of a stable, solid salt with an aliphatic, alicyclic or heterocyclic amine, and a solid, polymeric film forming material disposed upon an adsorbent bibulous carrier.
Inventors
- 👤 Vlastimil Svoboda
- 👤 Olga Celchovska
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:35
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/725; C12Q2326/00; C12Q2326/10; C12Q2326/14
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
72 words
Diagnostic test strip for the detection and semi-quantitative estimation of blood and hemoglobin in biological materials comprising a bibulous carrier impregnated with a composition of matter comprising (a) an acid buffer having a pH within the range of 2.5-5.0, (b) a chromogen, (c) an agent capable of enhancing the peroxidase activity of hemoglobin, (d) a wetting agent, (e) a solid, polymeric film-forming substance, and (f) an amine salt of an organic hydroperoxide.
Claim 2
Dependent Claim
72 words
Diagnostic test strip for the detection and semi-quantitative estimation of blood and hemoglobin in biological materials comprising a bibulous carrier impregnated with a composition of matter comprising (a) an acid buffer having a pH within the range of 2.5-5.0, (b) a chromogen, (c) an agent capable of enhancing the peroxidase activity of hemoglobin, (d) a wetting agent, (e) a solid, polymeric film-forming substance, and (f) an amine salt of an organic hydroperoxide.
Claim 3
Dependent Claim
62 words
Diagnostic test strip in accordance with claim 1, wherein the hydroperoxide salt comprises the reaction product of an organic hydroperoxide with an amine selected from the group consisting of aliphatic, alicyclic and heterocyclic amines having a pK of at least 8.0, said hydroperoxide being selected from the group consisting of tertiary butyl hydroperoxide, phenylisopropyl hydroperoxide, 4-methylphenylisopropyl hydroperoxide, phenyl-1, 4-diisopropyl-dihydroperoxide, 1-hydroxycyclohexyl-1-hydroperoxide, and 2,5-dimethylhexane-2,5-dihydroperoxide.
Claim 4
Dependent Claim
15 words
Diagnostic test strip in accordance with claim 1, wherein the bibulous carrier is filter paper.
Claim 5
Dependent Claim
24 words
Diagnostic test strip in accordance with claim 1, wherein the o-dianisidine chromogen is selected from the group consisting of benzidine, o-tolidine, guaiacol and 2,7-diaminofluorene.
Claim 6
Dependent Claim
24 words
Diagnostic test strip in accordance with claim 1, wherein the wetting agent is selected from the group consisting of anionic, non-ionic and cationic detergents.
Claim 7
Dependent Claim
31 words
Diagnostic test strip in accordance with claim 1, wherein the film-forming substance is selected from the group consisting of starch, polyvinyl alcohol, polyvinylpropionate, polyvinyl butyral, carboxymethylcellulose, polyvinyl pyrrolidone and polyethylene glycol.
Claim 8
Dependent Claim
104 words
Method for the preparation of a diagnostic test strip for the detection and semi-quantitative estimation of blood and hemoglobin in biological materials which comprises the steps of a. impregnating an adsorbent carrier with a solution of an acid buffer having a pH within the range of 2.5-5.0, and a chromogen, b. drying the resultant impregnated material at elevated temperatures, c. impregnating the dried carrier with a hydroperoxide salt comprising the reaction product of an organic hydroperoxide with an amine selected from the group consisting of aliphatic, alicyclic and heterocyclic amines having a pK of at least 8.0, and d. drying the resultant impregnated carrier.
Claim 9
Dependent Claim
104 words
Method for the preparation of a diagnostic test strip for the detection and semi-quantitative estimation of blood and hemoglobin in biological materials which comprises the steps of a. impregnating an adsorbent carrier with a solution of an acid buffer having a pH within the range of 2.5-5.0, and a chromogen, b. drying the resultant impregnated material at elevated temperatures, c. impregnating the dried carrier with a hydroperoxide salt comprising the reaction product of an organic hydroperoxide with an amine selected from the group consisting of aliphatic, alicyclic and heterocyclic amines having a pK of at least 8.0, and d. drying the resultant impregnated carrier.
References Cited (Backward Citations)
US3252762A
Cited
US3290117A
Cited
US3411887A
Cited
US3418083A
Cited
US3453180A
Cited
US3668076A
Cited
US3627697A
Cited
US3627698A
Cited
US3654179A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-04-11 | 1980-10-11 | 1981-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-04-11 | 1984-10-11 | 1985-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-04-11 | 1988-10-11 | 1989-04-12 | $7700.00 | Window Closed / Lapsed |