US4013514A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparing a reactor containing enzymes attached to dialdehyde cellulose
Assignee: Monsanto Co
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Filing: 1974-01-02
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Granted: 1977-03-22
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Expires: 1994-01-02
Abstract
Abstract Water-insoluble, biologically active conjugates for use in a reactor core of a flow-through reactor are prepared by covalently bonding an enzyme directly to a fibrous dialdehyde cellulose such as cotton. Preferably the fibrous cellulose is placed in the reactor core of a flow-through reactor before or after it is treated with periodic acid solution to convert glucose units thereof to units containing dialdehyde groups. Thereafter, an enzyme solution is passed through the resultant dialdehyde cellulose to bind the enzyme to the cellulose.
Inventors
- 👤 Bernard S. Wildi
- 👤 Lloyd E. Weeks
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:07
CPC Classifications
C; C12; C12H; C12H1/00; C12H1/003; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/12; C12P; C12P19/00; C12P19/14; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839; Y10S435/886; Y10S435/897
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
137 words
A method for producing a biologically active flow-through reactor which comprises the steps of reacting a liquid permeable fibrous cotton with a periodic acid solution for a time sufficient to oxidize 5 to 80 percent of the glucose units in the cotton to units containing dialdehyde groups; substantially freeing the fibrous dialdehyde cotton of excess periodic acid; placing the fibrous dialdehyde cotton in a liquid permeable, flow-through reactor to constitute an elongated reactor core, and passing a liquid containing an enzyme at a substantially neutral or alkaline pH through the fibrous dialdehyde cotton in the liquid permeable, flow-through reactor to react the enzyme through a primary amino group thereof not essential for biological activity directly with aldehyde groups of the cotton so as to form a carbonnitrogen double bond and thereby binding said enzyme to said core.
Claim 2
Independent Claim
137 words
A method for producing a biologically active flow-through reactor which comprises the steps of reacting a liquid permeable fibrous cotton with a periodic acid solution for a time sufficient to oxidize 5 to 80 percent of the glucose units in the cotton to units containing dialdehyde groups; substantially freeing the fibrous dialdehyde cotton of excess periodic acid; placing the fibrous dialdehyde cotton in a liquid permeable, flow-through reactor to constitute an elongated reactor core, and passing a liquid containing an enzyme at a substantially neutral or alkaline pH through the fibrous dialdehyde cotton in the liquid permeable, flow-through reactor to react the enzyme through a primary amino group thereof not essential for biological activity directly with aldehyde groups of the cotton so as to form a carbonnitrogen double bond and thereby binding said enzyme to said core.
Claim 3
Dependent Claim
11 words
Method in accordance with claim 1 wherein the enzyme is papain.
Claim 4
Independent Claim
140 words
A method for producing a flow-through reactor which comprises providing a liquid permeable biological reactor core containing an elongated liquid permeable mass of fibrous cotton in a flowthrough reactor; exposing the liquid permeable fibrous cotton in the flow-through reactor to a periodic acid solution for a time sufficient to convert from 5 to 80 percent of the glucose units in the cotton to dialdehyde groups; substantially freeing the dialdehyde fibrous cotton in the flow-through reactor of excess periodic acid, and passing a liquid containing an enzyme at a substantially neutral or alkaline pH through the fibrous dialdehyde cotton in the flow-through reactor to react the enzyme through a primary amino group thereof not essential for biological activity directly with aldehyde groups of the cotton so as to form a carbon-nitrogen double bond and thereby binding said enzyme to said core.
Claim 5
Independent Claim
140 words
A method for producing a flow-through reactor which comprises providing a liquid permeable biological reactor core containing an elongated liquid permeable mass of fibrous cotton in a flowthrough reactor; exposing the liquid permeable fibrous cotton in the flow-through reactor to a periodic acid solution for a time sufficient to convert from 5 to 80 percent of the glucose units in the cotton to dialdehyde groups; substantially freeing the dialdehyde fibrous cotton in the flow-through reactor of excess periodic acid, and passing a liquid containing an enzyme at a substantially neutral or alkaline pH through the fibrous dialdehyde cotton in the flow-through reactor to react the enzyme through a primary amino group thereof not essential for biological activity directly with aldehyde groups of the cotton so as to form a carbon-nitrogen double bond and thereby binding said enzyme to said core.
Claim 6
Dependent Claim
22 words
Method of claim 3 which further includes the step of passing a liquid containing a substrate for said enzyme through the reactor.
Claim 7
Dependent Claim
11 words
Method of claim 4 wherein the substrate containing liquid is beer.
Claim 8
Dependent Claim
11 words
Method in accordance with claim 3 wherein the enzyme is papain.
Claim 9
Dependent Claim
12 words
The method in accordance with claim 3 wherein the enzyme is alpha-amylase.
Claim 10
Dependent Claim
12 words
The method in accordance with claim 3 wherein the enzyme is subtilisin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-21 | 1980-09-20 | 1981-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-21 | 1984-09-20 | 1985-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-21 | 1988-09-20 | 1989-03-22 | $7700.00 | Window Closed / Lapsed |