US4004980A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme entrappment with cellulose acetate formulations
Assignee: Purdue Research Foundation
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Filing: 1975-03-25
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Granted: 1977-01-25
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Expires: 1995-03-25
Abstract
Abstract Enzymes are entrapped by preparing a polymer formulation containing cellulose triacetate and cellulose diacetate or glucose pentaacetate, adding an enzyme to the formulation, extruding the enzyme-containing formulation to form a solid matrix and soaking the solid matrix in an aqueous solution of acetone to swell and increase the permeability of the solid matrix.
Inventors
- 👤 Alden H. Emery
- 👤 Henry C. Lim
- 👤 Michael J. Kolarik
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:31
CPC Classifications
D; D01; D01F; D01F1/00; D01F1/02; C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12N11/10; C12N11/12; C12P; C12P19/00; C12P19/24
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
118 words
A method of forming an entrapped enzyme, said method comprising: providing cellulose triacetate in solid form; adding a second solid material to said cellulose triacetate, said second solid material being selected from the group consisting of cellulose diacetate and glucose pentaacetate; dissolving the cellulose triacetate and second solid material in a solvent; adding an enzyme to said cellulose triacetate and second solid material; extruding said dissolved cellulose triacetate and said second solid material through an orifice to form a solid matrix soluble in a swelling liquid and soaking said solid matrix in an aqueous solution of acetone and water containing between about 50 to 98% acetone for swelling said matrix to increase the permeability of the solid matrix.
Claim 2
Independent Claim
118 words
A method of forming an entrapped enzyme, said method comprising: providing cellulose triacetate in solid form; adding a second solid material to said cellulose triacetate, said second solid material being selected from the group consisting of cellulose diacetate and glucose pentaacetate; dissolving the cellulose triacetate and second solid material in a solvent; adding an enzyme to said cellulose triacetate and second solid material; extruding said dissolved cellulose triacetate and said second solid material through an orifice to form a solid matrix soluble in a swelling liquid and soaking said solid matrix in an aqueous solution of acetone and water containing between about 50 to 98% acetone for swelling said matrix to increase the permeability of the solid matrix.
Claim 3
Dependent Claim
38 words
The method of claim 1 wherein said second material is glucose pentaacetate and is added in solid form to said cellulose triacetate, said glucose pentaacetate being in the range of between 20 and 50% of said cellulose triacetate.
Claim 4
Dependent Claim
38 words
The method of claim 1 wherein said second material is cellulose diacetate and is added in solid form to said cellulose triacetate, said cellulose diacetate being in the range of between 20 and 80% of said cellulose triacetate.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein said cellulose triacetate and said second solid material are dissolved in methylene dichloride.
Claim 6
Dependent Claim
23 words
The method of claim 1 wherein said dissolved cellulose triacetate and second solid material are spun through an orifice to form a fiber.
Claim 7
Dependent Claim
23 words
The method of claim 1 wherein said dissolved cellulose triacetate and second solid material are cast through an orifice to form a film.
Claim 8
Dependent Claim
22 words
The method of claim 1 wherein said solid matrix is soaked in a mixture of acetone and water containing about 50% acetone.
Claim 9
Independent Claim
117 words
A method of forming an entrapped enzyme, said method comprising: providing a mixture in liquid form of cellulose triacetate and a second material selected from the group consisting of cellulose diacetate and glucose pentaacetate with both said cellulose triacetate and said second material having been dissolved from solid form in solvent; adding an enzyme to said cellulose triacetate and second solid material; extruding said dissolved cellulose triacetate and said second solid material through an orifice to form a solid matrix soluble in a swelling liquid; and soaking said solid matrix in an aqueous solution of acetone and water containing between about 50 and 98% acetone for swelling said matrix to increase the permeability of the solid matrix.
Claim 10
Independent Claim
117 words
A method of forming an entrapped enzyme, said method comprising: providing a mixture in liquid form of cellulose triacetate and a second material selected from the group consisting of cellulose diacetate and glucose pentaacetate with both said cellulose triacetate and said second material having been dissolved from solid form in solvent; adding an enzyme to said cellulose triacetate and second solid material; extruding said dissolved cellulose triacetate and said second solid material through an orifice to form a solid matrix soluble in a swelling liquid; and soaking said solid matrix in an aqueous solution of acetone and water containing between about 50 and 98% acetone for swelling said matrix to increase the permeability of the solid matrix.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-25 | 1980-07-26 | 1981-01-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-25 | 1984-07-26 | 1985-01-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-25 | 1988-07-26 | 1989-01-25 | $7700.00 | Window Closed / Lapsed |