US3999948A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic reagent holder and method
Assignee: Lonza Walkersville Inc
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Filing: 1975-11-03
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Granted: 1976-12-28
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Expires: 1995-11-03
Abstract
Abstract A diagnostic reagent holder specifically adapted for the detection of an unknown quantity of a biologically derived sample suitably by radioimmunoassay, fluorometric detection or by spectrophotometry. The holder comprises an elongate shaft with a support surface adjacent one end of the shaft. Diagnostic reagent is covalently attached to a self-supporting film such as a disc and firmly secured to the support. The diagnostic reagent, e.g., antibody, is stirred during incubation in contact with the sample to be detected, e.g., antigen, in solution. In a "sandwich" technique labelled substance (e.g., fluorochrome-labelled antibody) is reacted with the sample on the film followed by thorough washing. Then, the holder is slid into a novel viewing housing which precisely positions the film at a window for viewing as with a fluorometer. To avoid loss of labelled substance during handling, and especially during positioning in the viewing housing, one or more protective protrusions are provided around the periphery of the support surface.
Inventors
- 👤 Fred H. Deindoerfer
- 👤 Roland Jang
- 👤 H. Theodore Rudow
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/811
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
103 words
A viewing housing for receiving a diagnostic reagent holder, said holder comprising an elongate shaft connected to a reagent containing surface and which is placed in a predetermined repeatable position in the housing, said viewing housing comprising housing wall means including a front wall portion, means defining a reagent holder slot in said housing, stop means for limiting penetration of the reagent holder a predetermined distance along said reagent holder slot defining means, and a reagent surface window opening through said front wall into said slot portion defining means for viewing the reagent containing surface when the reagent holder contacts said stop means.
Claim 2
Dependent Claim
103 words
A viewing housing for receiving a diagnostic reagent holder, said holder comprising an elongate shaft connected to a reagent containing surface and which is placed in a predetermined repeatable position in the housing, said viewing housing comprising housing wall means including a front wall portion, means defining a reagent holder slot in said housing, stop means for limiting penetration of the reagent holder a predetermined distance along said reagent holder slot defining means, and a reagent surface window opening through said front wall into said slot portion defining means for viewing the reagent containing surface when the reagent holder contacts said stop means.
Claim 3
Dependent Claim
27 words
A viewing housing as in claim 1 together with means for limiting lateral movement along said track means to define at least two precise predetermined lateral positions.
Claim 4
Dependent Claim
24 words
A viewing housing as in claim 1 together with a source of light and a receiver of excited light both aligned with said window.
Claim 5
Dependent Claim
33 words
A viewing housing as in claim 1 together with means defining a cuvette slot, with a cuvette viewing opening in said front wall portion laterally spaced from said reagent holder slot defining means.
Claim 6
Dependent Claim
40 words
A viewing housing as in claim 4 together with a base member adapted for stationary mounting to a detection assembly, and cooperating track means mounted to said viewing housing and base member for sliding lateral movement of said viewing housing.
Claim 7
Dependent Claim
30 words
A viewing housing as in claim 1 together with setting means for urging the reagent holder against a surface of said viewing housing when the holder contacts said stop means.
Claim 8
Dependent Claim
35 words
A viewing housing as in claim 6 in which said setting means urges said reagent holder toward said slot defining means, said setting means comprising a resiliently mounted setting member projecting toward said reagent holder.
Claim 9
Dependent Claim
40 words
A viewing housing as in claim 6 in which said reagent holder is disposed in said reagent holder slot defining means and in contact with said stop means and setting means with said reagent support surface visible in said window.
Claim 10
Dependent Claim
68 words
An elongate diagnostic reagent holder having front and rear sides and comprising an elongate shaft, mounting means coupled to said shaft, self-supporting film bearing a diagnostic reagent firmly retained toward the end of said shaft by said mounting means on said front side, said mounting means comprising means defining a retaining groove at one end of said shaft, said film being retained in registry with said retaining groove.
Claim 11
Dependent Claim
68 words
An elongate diagnostic reagent holder having front and rear sides and comprising an elongate shaft, mounting means coupled to said shaft, self-supporting film bearing a diagnostic reagent firmly retained toward the end of said shaft by said mounting means on said front side, said mounting means comprising means defining a retaining groove at one end of said shaft, said film being retained in registry with said retaining groove.
Claim 12
Dependent Claim
58 words
An elongate diagnostic reagent holder having front and rear sides and comprising an elongate shaft, mounting means coupled to said shaft, self-supporting film bearing a diagnostic reagent firmly retained toward the end of said shaft by said mounting means on said front side, and a locating means to assist registry thereof with a cooperating portion of a housing.
Claim 13
Dependent Claim
58 words
An elongate diagnostic reagent holder having front and rear sides and comprising an elongate shaft, mounting means coupled to said shaft, self-supporting film bearing a diagnostic reagent firmly retained toward the end of said shaft by said mounting means on said front side, and a locating means to assist registry thereof with a cooperating portion of a housing.
Claim 14
Dependent Claim
21 words
The holder of claim 10 in which said locating means comprises a recess and said cooperating housing portion comprises a projection.
Claim 15
Dependent Claim
21 words
The holder of claim 10 in which said locating means comprises a projection and said cooperating housing portion comprises a recess.
Claim 16
Dependent Claim
72 words
An elongate diagnostic reagent holder having front and rear sides and comprising an elongate shaft, mounting means coupled to said shaft, self-supporting film bearing a diagnostic reagent firmly retained toward the end of said shaft by said mounting means on said front side, and a pivot extension projecting from said holder at the film end and sufficiently aligned with said shaft to form a pivotal axis during stirring to facilitate liquid flow.
Claim 17
Dependent Claim
72 words
An elongate diagnostic reagent holder having front and rear sides and comprising an elongate shaft, mounting means coupled to said shaft, self-supporting film bearing a diagnostic reagent firmly retained toward the end of said shaft by said mounting means on said front side, and a pivot extension projecting from said holder at the film end and sufficiently aligned with said shaft to form a pivotal axis during stirring to facilitate liquid flow.
Claim 18
Dependent Claim
72 words
A continuous diagnostic reagent holder having front and rear sides and comprising an elongate shaft and a support surface adjacent one end of said shaft, a self-supporting film firmly adhered to the front side of said support surface, a diagnostic reagent coating affixed to said film, and protrusion means disposed externally adjacent the periphery of the film and projecting outwardly from the film a sufficient distance to protect the film from abrasion.
Claim 19
Dependent Claim
72 words
A continuous diagnostic reagent holder having front and rear sides and comprising an elongate shaft and a support surface adjacent one end of said shaft, a self-supporting film firmly adhered to the front side of said support surface, a diagnostic reagent coating affixed to said film, and protrusion means disposed externally adjacent the periphery of the film and projecting outwardly from the film a sufficient distance to protect the film from abrasion.
Claim 20
Dependent Claim
23 words
The holder of claim 14 in which said protrusion means comprises a protective rim around substantially all of the periphery of said film.
Claim 21
Dependent Claim
44 words
The holder of claim 14 together with an enlarged handle portion at the opposite end of the shaft from the film and of sufficient dimension transverse to the shaft to facilitate stirring a liquid with the holder and to support a sample identification label.
Claim 22
Dependent Claim
22 words
The holder of claim 14 in which said diagnostic reagent comprises a reagent selected from the group consisting of antigen or antibody.
Claim 23
Dependent Claim
17 words
The holder of claim 17 in which said antigen or antibody bears a fluorescent or phorphorescent label.
Claim 24
Dependent Claim
15 words
The holder of claim 14 in which said film comprises a disc bearing diagnostic reagent.
Claim 25
Dependent Claim
14 words
The holder of claim 19 in which said film is essentially impermeable to liquid.
Claim 26
Dependent Claim
20 words
The holder of claim 19 in which said diagnostic reagent is covalently attached to an external surface of the disc.
References Cited (Backward Citations)
US2749797A
Cited
US3443903A
Cited
US3507269A
Cited
US3526480A
Cited
US3881993A
Cited
US3904373A
Cited
US3907503A
Cited
US3901657A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-12-28 | 1980-06-28 | 1980-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-12-28 | 1984-06-28 | 1984-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-12-28 | 1988-06-28 | 1988-12-28 | $7700.00 | Window Closed / Lapsed |