US3989596A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Aggregate of dried flocculated cells
Assignee: RJ Reynolds Tobacco Co
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Filing: 1975-10-14
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Granted: 1976-11-02
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Expires: 1995-10-14
Abstract
Abstract Microbial cells having active enzymes associated therewith are subjected to flocculation conditions to produce a flocculated whole cell aggregate that is subsequently dried. The dried aggregate is useful in effecting enzyme-catalyzed chemical transformations. Substrates are brought into contact with the flocculated cell aggregates where they undergo chemical transformations in the presence of active enzymes associated with the cells.
Inventors
- 👤 Margaret E. Long
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:14
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/12; C12N; C12N1/00; C12N1/005; C12P19/00; C12P19/24
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
62 words
An enzyme-containing aggregate capable of effecting enzymatic transformation of a substrate contacted therewith comprising microbial cells having said enzyme associated therewith flocculated with at least one percent by weight based on the wet weight of said cells of a synthetic polyelectrolyte flocculating agent wherein said aggregate is dried and said agent is selected from the group consisting of anionic and cationic polyelectrolytes.
Claim 2
Dependent Claim
62 words
An enzyme-containing aggregate capable of effecting enzymatic transformation of a substrate contacted therewith comprising microbial cells having said enzyme associated therewith flocculated with at least one percent by weight based on the wet weight of said cells of a synthetic polyelectrolyte flocculating agent wherein said aggregate is dried and said agent is selected from the group consisting of anionic and cationic polyelectrolytes.
Claim 3
Dependent Claim
13 words
An aggregate according to claim 1 which has been extruded prior to drying.
Claim 4
Dependent Claim
10 words
An aggregate according to claim 1 which has been milled.
Claim 5
Dependent Claim
10 words
An aggregate according to claim 3 which has been sieved.
Claim 6
Dependent Claim
16 words
An aggregate according to claim 1 in which the aggregate is dried and granular in form.
Claim 7
Dependent Claim
48 words
An enzyme-containing aggregate capable of effecting enzymatic transformation of a substrate contacted therewith comprising microbial cells having a glucose-isomerizing enzyme associated therewith flocculated with a synthetic polyelectrolyte flocculating agent wherein said aggregate is dried and said agent is selected from the group consisting of anionic and cationic polyelectrolytes.
Claim 8
Dependent Claim
48 words
An enzyme-containing aggregate capable of effecting enzymatic transformation of a substrate contacted therewith comprising microbial cells having a glucose-isomerizing enzyme associated therewith flocculated with a synthetic polyelectrolyte flocculating agent wherein said aggregate is dried and said agent is selected from the group consisting of anionic and cationic polyelectrolytes.
Claim 9
Dependent Claim
16 words
An aggregate according to claim 6 in which the microbial cells belong to the genus Arthrobacter.
Claim 10
Dependent Claim
16 words
An aggregate according to claim 6 in which the microbial cells belong to the genus Streptomyces.
Claim 11
Dependent Claim
16 words
A dried aggregate according to claim 6 which contains both a cationic and an anionic polyelectrolyte.
Claim 12
Dependent Claim
14 words
A dried aggregate according to claim 6 which has been extruded prior to drying.
Claim 13
Dependent Claim
11 words
A dried aggregate according to claim 6 which has been milled.
Claim 14
Dependent Claim
11 words
A dried aggregate according to claim 11 which has been sieved.
Claim 15
Dependent Claim
13 words
An aggregate according to claim 6 which is dried and granular in form.
Claim 16
Dependent Claim
46 words
An enzyme-containing aggregate capable of effecting enzymatic transformation of a substrate contacted therewith comprising microbial cells having glucose isomerase associated therewith flocculated with a synthetic polyelectrolyte flocculating agent selected from the group consisting of anionic and cationic polyelectrolytes, and wherein said aggregate is extruded and dried.
Claim 17
Dependent Claim
46 words
An enzyme-containing aggregate capable of effecting enzymatic transformation of a substrate contacted therewith comprising microbial cells having glucose isomerase associated therewith flocculated with a synthetic polyelectrolyte flocculating agent selected from the group consisting of anionic and cationic polyelectrolytes, and wherein said aggregate is extruded and dried.
Claim 18
Dependent Claim
16 words
A dried aggregate according to claim 1 which contains both a cationic and an anionic polyelectrolyte.
Claim 19
Dependent Claim
13 words
An aggregate according to claim 2 which is dried and granular in form.
Claim 20
Dependent Claim
17 words
A dried aggregate according to claim 1 in which the microbial cells belong to the genus Arthrobacter.
Claim 21
Dependent Claim
17 words
A dried aggregate according to claim 1 in which the microbial cells belong to the genus Streptomyces.
Claim 22
Dependent Claim
13 words
A dried aggregate according to claim 1 which has been milled and sieved.
Claim 23
Dependent Claim
15 words
An aggregate according to claim 6 which contains both a cationic and an anionic polyelectrolyte.
Claim 24
Dependent Claim
16 words
An aggregate according to claim 7 in which the microbial cells belong to the genus Arthrobacter.
Claim 25
Dependent Claim
13 words
An aggregate according to claim 8 which is dried and granular in form.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-11-02 | 1980-05-03 | 1980-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-11-02 | 1984-05-03 | 1984-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-11-02 | 1988-05-03 | 1988-11-02 | $7700.00 | Window Closed / Lapsed |