US3988429A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Kit for the rapid preparation of 99m Tc red blood cells
Assignee: Energy Research and Development Administration ERDA
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Filing: 1975-04-16
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Granted: 1976-10-26
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Expires: 1995-04-16
Abstract
Abstract A method and sample kit for the preparation of 99m Tc-labeled red blood cells in a closed, sterile system. A partially evacuated tube, containing a freeze-dried stannous citrate formulation with heparin as an anticoagulant, allows whole blood to be automatically drawn from the patient. The radioisotope is added at the end of the labeling sequence to minimize operator exposure. Consistent 97% yields in 20 minutes are obtained with small blood samples. Freeze-dried kits have remained stable after five months.
Inventors
- 👤 Powell Richards
- 👤 Terry D. Smith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:42
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/12; A61K51/1203; A61K2123/00
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
132 words
The method of labeling red blood cells with 99m Tc comprising the steps of a. supplying a sample of blood to a container having therein an anticoagulant, a freeze-dried stannous reagent consisting of stannous citrate present in the range of about 0.10 to 0.25 μg of the stannous ion for each ml. of the blood sample, and a metal chelate stabilizer to provide bulk; b. incubating the resulting mixture until all of the solids are dissolved; c. adding sterile saline solution; d. centrifuging the contents of said container to form packed red blood cells; e. withdrawing some of said packed red blood cells; f. mixing said packed red blood cells with previously prepared saline solution of Na 99m TcO 4 ; and g. incubating the technetium-red blood cells mixture with gentle mixing.
Claim 2
Independent Claim
132 words
The method of labeling red blood cells with 99m Tc comprising the steps of a. supplying a sample of blood to a container having therein an anticoagulant, a freeze-dried stannous reagent consisting of stannous citrate present in the range of about 0.10 to 0.25 μg of the stannous ion for each ml. of the blood sample, and a metal chelate stabilizer to provide bulk; b. incubating the resulting mixture until all of the solids are dissolved; c. adding sterile saline solution; d. centrifuging the contents of said container to form packed red blood cells; e. withdrawing some of said packed red blood cells; f. mixing said packed red blood cells with previously prepared saline solution of Na 99m TcO 4 ; and g. incubating the technetium-red blood cells mixture with gentle mixing.
Claim 3
Dependent Claim
12 words
The method of claim 1 in which the anticoagulant is sodium heparin.
Claim 4
Dependent Claim
16 words
The method of claim 2 in which the metal chelate stabilizer includes sodium citrate and dextrose.
Claim 5
Dependent Claim
68 words
A kit for use in the labeling of red blood cells with 99m Tc comprising a partially evacuated container with a needle for the insertion of a blood sample, said container having within under sterile conditions a dry mixture of sodium heparin, stannous citrate in the amount of about 0.10 to 0.25 μg of stannous ion per ml. of blood sample to be taken, sodium citrate, and dextrose.
Claim 6
Dependent Claim
68 words
A kit for use in the labeling of red blood cells with 99m Tc comprising a partially evacuated container with a needle for the insertion of a blood sample, said container having within under sterile conditions a dry mixture of sodium heparin, stannous citrate in the amount of about 0.10 to 0.25 μg of stannous ion per ml. of blood sample to be taken, sodium citrate, and dextrose.
References Cited (Backward Citations)
US3725295A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-10-26 | 1980-04-26 | 1980-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-10-26 | 1984-04-26 | 1984-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-10-26 | 1988-04-26 | 1988-10-26 | $7700.00 | Window Closed / Lapsed |