Patent US5304214A successfully refreshed from Google Patents.
US3975161A
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US
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Biological diagnostic test strip
Assignee: Lachema
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Filing: 1975-11-06
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Granted: 1976-08-17
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Expires: 1995-11-06
Abstract
Abstract Diagnostic test strips for the qualitative detection and semi-quantitative estimation of blood and hemoglobin in biological products include a reagent area comprising an organic hydroperoxide or salt thereof, a chromagen and a novel agent capable of accelerating the peroxidase activity of hemoglobin. The accelerating agent is an aromatic hydrocarbon of the general formula WHEREIN R1 - R7 are hydrogen or alkyl or alkoxy groups having 1-2 carbon atoms. The resultant test strips evidence marked enhancement in sensitivity as compared with prior art materials.
Inventors
- 👤 Vlastimil Svoboda
- 👤 Olga Celechovska
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/725
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
108 words
Diagnostic test strip for the qualitative detection and the semi-quantitative estimation of blood and hemoglobin in biological materials comprising a bibulous carrier impregnated with a composition of matter comprising (a) an organic hydroperoxide or salt thereof, (b) an acid buffer, (c) a chromogen, (d) a wetting agent, (e) a solid polymeric film-forming natural or synthetic substance and (f) an agent capable of accelerating the peroxidase activity of hemoglobin characterized in that said agent is of the general formula ##SPC3## wherein R 1 - R 7 are selected from the group consisting of hydrogen, alkyl groups having from 1-2 carbon atoms and alkoxy groups having from 1-2 carbon atoms.
Claim 2
Dependent Claim
108 words
Diagnostic test strip for the qualitative detection and the semi-quantitative estimation of blood and hemoglobin in biological materials comprising a bibulous carrier impregnated with a composition of matter comprising (a) an organic hydroperoxide or salt thereof, (b) an acid buffer, (c) a chromogen, (d) a wetting agent, (e) a solid polymeric film-forming natural or synthetic substance and (f) an agent capable of accelerating the peroxidase activity of hemoglobin characterized in that said agent is of the general formula ##SPC3## wherein R 1 - R 7 are selected from the group consisting of hydrogen, alkyl groups having from 1-2 carbon atoms and alkoxy groups having from 1-2 carbon atoms.
Claim 3
Dependent Claim
14 words
Diagnostic test strip in accordance with claim 1, wherein the accelerating agent is 3-methyl-isoquinoline.
Claim 4
Dependent Claim
15 words
Diagnostic test strip in accordance with claim 1, wherein the accelerating agent is isoquinoline hydrochloride.
Claim 5
Dependent Claim
14 words
Diagnostic test strip in accordance with claim 1, wherein the accelerating agent is 3-methyl-6-methoxyisoquinoline.
Claim 6
Dependent Claim
15 words
Diagnostic test strip in accordance with claim 1, wherein the bibulous carrier is filter paper.
Claim 7
Dependent Claim
26 words
Diagnostic test strip in accordance with claim 1, wherein the hydroperoxide is selected from the group consisting of cumene hydroperoxide, para-menthane-hydroperoxide, 1,4-diisopropylbenzene-1,4-dihydroperoxide, 2,5-dimethylhexane 2,5-dihydroperoxide, or 1-hydroxycyclohexane-1-hydroperoxide.
Claim 8
Dependent Claim
26 words
Diagnostic test strip in accordance with claim 6, wherein the acid buffer is capable of maintaining the pH in the strip within the range of 3-7.
Claim 9
Dependent Claim
29 words
Diagnostic test strip in accordance with claim 7, wherein the accelerating agent is employed in an amount ranging from 0.05 - 2.5 grams per 100 ml of impregnating composition.
Claim 10
Dependent Claim
29 words
Diagnostic test strip in accordance with claim 8, wherein the accelerating agent is employed in an amount ranging from 0.3 - 1.5 grams per 100 ml of impregnating composition.
References Cited (Backward Citations)
US3290117A
Cited
US3853472A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-17 | 1980-02-16 | 1980-08-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-17 | 1984-02-16 | 1984-08-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-17 | 1988-02-16 | 1988-08-17 | $7700.00 | Window Closed / Lapsed |