US3929581A
Expired / Lapsed
US
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Quantitative determination of blood ammonia
Assignee: Roche Diagnostics GmbH
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Filing: 1974-05-17
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Granted: 1975-12-30
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Expires: 1994-05-17
Abstract
Abstract Blood ammonia is quantitatively determined by contacting a sample suspected of containing blood ammonia, with glutamate dehydrogenase, Alpha -ketoglutarate, and, as a coenzyme in the reduced state, nicotinamide-adenine-dinucleotide phosphate in reduced form.
Inventors
- 👤 Fonseca-Wollheim Friedrich Da
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:28
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Q1/58
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
35 words
PROCESS FOR THE QUANTITATIVE DETERMINATION OF BLOOD AMMONIA WHICH COMPRISES CONTACTING A SAMPLE SUSPECTED OF CONTAINING BLOOD AMMONIA WITH GLUTAMATE DEHYDROGENASE, A-KETOGLUTARATE, AND, AS A COENZYME IN THE REDUCED STATE, NICOTINAMIDEADENINE-DINUCLEOTIDE PHOSPHATE IN REDUCED FORM.
Claim 2
Dependent Claim
35 words
PROCESS FOR THE QUANTITATIVE DETERMINATION OF BLOOD AMMONIA WHICH COMPRISES CONTACTING A SAMPLE SUSPECTED OF CONTAINING BLOOD AMMONIA WITH GLUTAMATE DEHYDROGENASE, A-KETOGLUTARATE, AND, AS A COENZYME IN THE REDUCED STATE, NICOTINAMIDEADENINE-DINUCLEOTIDE PHOSPHATE IN REDUCED FORM.
Claim 3
Dependent Claim
6 words
5 to 50 U glutamate dehydrogenase
Claim 4
Dependent Claim
6 words
5 to 50 U glutamate dehydrogenase
Claim 5
Dependent Claim
19 words
Process as claimed in claim 1, wherein the determination is carried out in the additional presence of adenosine diphosphate.
Claim 6
Dependent Claim
3 words
glutamate dehydrogenase; and
Claim 7
Dependent Claim
3 words
glutamate dehydrogenase; and
Claim 8
Dependent Claim
1 words
NADPH.
Claim 9
Dependent Claim
1 words
NADPH.
Claim 10
Dependent Claim
20 words
Process as claimed in claim 1, wherein the determination is carried out in a buffer of pH 7.0 to 9.5.
Claim 11
Dependent Claim
10 words
0.1 to 0.5 Mu Mol NADPH or a multiple thereof.
Claim 12
Dependent Claim
10 words
0.1 to 0.5 Mu Mol NADPH or a multiple thereof.
Claim 13
Dependent Claim
20 words
Process as claimed in claim 3, wherein the determination is carried out in a buffer of pH 8.0 to 9.0.
Claim 14
Dependent Claim
20 words
Process as claimed in claim 4, wherein the determination is carried out in a buffer of pH 8.5 to 8.7.
Claim 15
Dependent Claim
18 words
Process as claimed in claim 5, wherein the determination is carried out in a buffer of pH 8.6.
Claim 16
Dependent Claim
20 words
Process as claimed in claim 3, wherein the buffer used is one of tris buffer, triethanolamine buffer and phosphate buffer.
Claim 17
Dependent Claim
13 words
Reagent combination for carrying out the process as claimed in claim 1, comprising:
Claim 18
Dependent Claim
13 words
Reagent combination as claimed in claim 8, containing ADP dissolved in said buffer.
Claim 19
Dependent Claim
8 words
Reagent combination as claimed in claim 8, comprising:
Claim 20
Dependent Claim
39 words
Reagent combination according to claim 9, wherein the third component comprises: 0.1 to 0.5 Mu Mol NADPH 10 to 50 mg serum albumen 1 to 5 Mu Mol sodium bicarbonate 5 to 60 mg dextran or a multiple thereof.
Claim 21
Dependent Claim
16 words
Reagent combination as claimed in claim 10 also containing 2 to 30 mM alkali metal azide.
References Cited (Backward Citations)
US3527674A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-29 | 1979-06-30 | 1979-12-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-29 | 1983-06-30 | 1983-12-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-29 | 1987-06-30 | 1987-12-30 | $7700.00 | Window Closed / Lapsed |