US3926736A
Expired / Lapsed
US
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Enzymatic ethanol assay
Assignee: CNA Holdings LLC
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Filing: 1974-08-30
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Granted: 1975-12-16
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Expires: 1994-08-30
Abstract
Abstract This invention provides an improved enzymatic assay for ethanol in body fluids, whereby a single substance acts simultaneously as a buffer and as a trapping agent for one of the products of the enzymatic reaction.
Inventors
- 👤 Giovanni Bucolo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:51
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
80 words
A COMPOSITION OF MATTER AS A REAGENT IN DETERMINING THE ETHANOL CONTENT OF BODY FLUIDS CONSISTING ESSENTIALLY OF: A. THE ENZYME ALCOHOL DEHYDROGENASE; B. THE COENZYME NICTINAMIDE ADENINE DINUCLEOTIDE; C. A BUFFER/ACTEALDEHYDR-TRAPPING AGENT SELECTED FROM THE GROUP CONSISTING OF 2-AMINO-2-HYDROXYMETHYL-1,3PROPANEDIOL, 2-AMINO-2-METHYL-1,3-PROPANEDIOL AND MIXTURES THEREOF; D. AM ACID IN QUANTITY SUFFICIENT TO LOWER THE PH OF AN AQUEOUS SOLUTION OF SAID MIXTURE TO WITHIN THE RANGE OF ABOUT 8.8 TO 9.2; SAID MIXTURE BEING FREE OF ANY OTHER ACETALDEHYDE TRAPPING AGENT.
Claim 2
Dependent Claim
80 words
A COMPOSITION OF MATTER AS A REAGENT IN DETERMINING THE ETHANOL CONTENT OF BODY FLUIDS CONSISTING ESSENTIALLY OF: A. THE ENZYME ALCOHOL DEHYDROGENASE; B. THE COENZYME NICTINAMIDE ADENINE DINUCLEOTIDE; C. A BUFFER/ACTEALDEHYDR-TRAPPING AGENT SELECTED FROM THE GROUP CONSISTING OF 2-AMINO-2-HYDROXYMETHYL-1,3PROPANEDIOL, 2-AMINO-2-METHYL-1,3-PROPANEDIOL AND MIXTURES THEREOF; D. AM ACID IN QUANTITY SUFFICIENT TO LOWER THE PH OF AN AQUEOUS SOLUTION OF SAID MIXTURE TO WITHIN THE RANGE OF ABOUT 8.8 TO 9.2; SAID MIXTURE BEING FREE OF ANY OTHER ACETALDEHYDE TRAPPING AGENT.
Claim 3
Dependent Claim
29 words
The composition of claim 1 wherein said composition is present in the form of two moieties, one containing said coenzyme and the other the balance of the recited components.
Claim 4
Dependent Claim
12 words
The composition of claim 1 which includes a heavy metal sequestering agent.
Claim 5
Dependent Claim
20 words
The composition of claim 3 in which said sequestering agent is ethylene diamine tetra-acetic acid, or a sodium salt thereof.
Claim 6
Dependent Claim
39 words
An assay solution useful in the determination of the ethanol content of body fluids consisting essentially of the composition of claim 1 dissolved in sufficient water to provide a concentration of said buffer/trapping agent of at least 0.3 molar.
Claim 7
Dependent Claim
33 words
A composition in accordance with claim 1 wherein, for each 6.99 millimoles of said buffer/trapping agent, 16 - 18 mg of NAD; and from 200 - 220 I.U. of alcohol dehydrogenase are present.
Claim 8
Dependent Claim
40 words
An assay solution useful in the determination of the ethanol content of boDy fluids consisting essentially of the composition of claim 6 dissolved in sufficient water to provide a concentration of said buffer/trapping agent of approximately at least 0.3 molar.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-15 | 1979-06-16 | 1979-12-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-15 | 1983-06-16 | 1983-12-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-15 | 1987-06-16 | 1987-12-16 | $7700.00 | Window Closed / Lapsed |