US3925545A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing adsorbed vaccines
Assignee: Institut Pasteur
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Filing: 1973-03-20
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Granted: 1975-12-09
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Expires: 1993-03-20
Abstract
Abstract An improvement in the preparation of a vaccine adsorbed by a gel of calcium phosphate, by contacting an antigen with an aqueous gel obtained by reacting an aqueous solution of dibasic sodium phosphate with an aqueous solution of calcium chloride, wherein the calcium chloride solution is poured as rapidly as possible, within less than 3 minutes, into said phosphate solution, in a proportion substantially equal to 1 mol PO4HNa2 for 1 mol CaCl2, under stirring; the mixture thus obtained is still stirred while its pH is adjusted to a value very near 7, and the gel formed is subsequently decanted and washed.
Inventors
- 👤 Edgar Hans Relyveld
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 21:59
CPC Classifications
A; A61; A61K; A61K47/00; A61K47/02; A61K9/00; A61K9/0012; A61K9/0019; A61K39/00; A61K2039/555; A61K2039/55505; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
123 words
IN THE METHOD OF PREPARING A COMPOSITION INCLUDING A VACCINE IN AN ADSORBED STATE, BY CONTACTING AN AQUEOUS VACCINE WITH AN AQUEOUS GEL FORMED BY POURING A CALCIUM CHLORIDE AQUEOUS SOLUTION HAVING A CONCENTRATION OF 0.01 M TO 0.5 M INTO AN AQUEOUS SOLUTION OF DIBASIC SODIUM PHOSPHATE HAVING A CONCENTRATION OF 0.01 M TO 0.5 M, IN A PROPORTION SUBSTANTIALLY EQUAL TO 1 MOL OF PO4HNA2 PER MOL OF CACL2, UNDER STIRRING, THE MIXTURE THUS OBTAINED BEING FURTHER STIRRED WHILE ITS PH IS ADJUSTED TO A VALUE OF 6.8 TO 7.2, AND THE GEL FORMED BEING SUBSEQUENTLY DECANTED AND WASHED, THE IMPROVEMENT WHICH COMPRISES CARRYING OUT SAID POURING OF THE CALCIUM CHLORIDE SOLUTION AS RAPIDLY AS POSSIBLE WITHIN LESS THAN 3 MINUTES.
Claim 2
Dependent Claim
123 words
IN THE METHOD OF PREPARING A COMPOSITION INCLUDING A VACCINE IN AN ADSORBED STATE, BY CONTACTING AN AQUEOUS VACCINE WITH AN AQUEOUS GEL FORMED BY POURING A CALCIUM CHLORIDE AQUEOUS SOLUTION HAVING A CONCENTRATION OF 0.01 M TO 0.5 M INTO AN AQUEOUS SOLUTION OF DIBASIC SODIUM PHOSPHATE HAVING A CONCENTRATION OF 0.01 M TO 0.5 M, IN A PROPORTION SUBSTANTIALLY EQUAL TO 1 MOL OF PO4HNA2 PER MOL OF CACL2, UNDER STIRRING, THE MIXTURE THUS OBTAINED BEING FURTHER STIRRED WHILE ITS PH IS ADJUSTED TO A VALUE OF 6.8 TO 7.2, AND THE GEL FORMED BEING SUBSEQUENTLY DECANTED AND WASHED, THE IMPROVEMENT WHICH COMPRISES CARRYING OUT SAID POURING OF THE CALCIUM CHLORIDE SOLUTION AS RAPIDLY AS POSSIBLE WITHIN LESS THAN 3 MINUTES.
Claim 3
Dependent Claim
18 words
Method according to claim 1, wherein said pouring is effected within a time of less than one minute.
Claim 4
Dependent Claim
20 words
Method according to claim 1, wherein each of said aqueous solutions has a concentration of 0.05 M to 0.1 M.
Claim 5
Dependent Claim
86 words
Method according to claim 1, wherein the decantation and washing of the gel formed is carried out by removing the supernatant layer of clear liquid which forms after the gel has been left to stand until 80 to 90% of its total volume has separated as supernatant clear layer, the period of standing and the subsequent removal of clear liquid being repeated two to four times, while the clear liquid removed is replaced by a sterile aqueous solution having 4 to 9 grams NaCl per liter.
Claim 6
Dependent Claim
96 words
Method according to claim 1, wherein a first aqueous vaccine is mixed with the gel formed, the mixture is subjected to standing, and the clear supernatant liquid is separated and replaced by a sterile aqueous solution having 4 to 9 grams NaCl per liter, and then a second aqueous vaccine, immunologically different from the first one, is mixed with the gel thus treated, and the resulting mixture is again subjected to standing, removing of the clear supernatant liquid and replacing of the same by a sterile aqueous solution having 4 to 9 grams NaCl per liter.
Claim 7
Dependent Claim
37 words
Method according to claim 1, wherein the contacting of said aqueous vaccine with said gel is effected by mixing the vaccine with the solution of dibasic sodium phosphate before the solution of calcium chloride is poured thereinto.
Claim 8
Dependent Claim
11 words
The method of claim 1, wherein the vaccine is diphteria anatoxin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-08 | 1979-06-09 | 1979-12-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-08 | 1983-06-09 | 1983-12-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-08 | 1987-06-09 | 1987-12-09 | $7700.00 | Window Closed / Lapsed |