US3915802A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Dialysis process and apparatus
Assignee: Pharmacia and Upjohn Co
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Filing: 1974-08-19
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Granted: 1975-10-28
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Expires: 1994-08-19
Abstract
Abstract A new dialysis method and apparatus is described for separating, accumulating, and recovering cycloheximide from a culture medium wherein Streptomyces griseus is producing the antibiotic. The new method and apparatus utilizes an aqueous pick-up medium (a diffusate) which is flowed through tubular structure of a semipermeable membrane means through which membrane cycloheximide will diffuse from a fermentation culture medium producing the same. The flowing diffusate, having accumulated cycloheximide in its passage through the membrane structure, is discharged into a water-immiscible organic solvent where the cycloheximide is extracted from the diffusate because of its greater solubility in the organic solvent. The extracted aqueous medium collects in an aqueous phase separate from the organic solvent phase. The aqueous phase is drawn off (preferably stripped of any dissolved organic solvent) and returned to the semi-permeable membrane means for further separation and accumulation of diffusing cycloheximide and discharge into the extracting solvent. The same procedure is applicable to other biochemicals produced by microbial cultures.
Inventors
- 👤 Leo A Kominek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 08:34
CPC Classifications
B; B01; B01D; B01D61/00; B01D61/24; B01D61/28; B01D63/00; B01D63/06; B01D71/00; B01D71/06; B01D71/08; B01D71/12; C; C12; C12M; C12M29/00; C12M29/04; C12M47/00; C12M47/10; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/886; Y10S435/897
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
106 words
THE PROCESS FOR SEPARATING AND RECOVERING A DESIRED BIOCHEMICAL PRODUCT FROM A FERMENTATION CULTURE MEDIUM WHEREIN THE PRODUCT IS BEING PRODUCED, WHICH COMPRISES: CONTACTING A TUBULAR, SEMI-PERMEABLE MEMBRANCE MEANS WITH THE CULTURE MEDIUM SO THAT THE PRODUCT DIFFUSES ACROSS THE MEMBRANE INTO AN AQUEOUS MEDIUM FLOWING IN THE MEMBRANE MEANS, EXTRACTING OF THE PRODUCT FROM THE AQUEOUS MEDIUM BY A WATERIMMISCIBLE ORGANIC SOLVENT, ACCUMULATING OF THE EXTRACTED AQUEOUS MEDIUM IN AN AQUEOUS PHASE, WITH THE BIOCHEMICAL PRODUCT REMAINING IN THE ORGANIC SOLVENT PHASE AND WITHDRAWING THE AQUEOUS PHASE FOR PURPOSES OF OPTIONALLY STRIPPING ORGANIC SOLVENT FROM IT, AND RECYCLING IT TO THE TUBULAR, SEMIPERMEABLE MEMBRANE MEANS.
Claim 2
Dependent Claim
106 words
THE PROCESS FOR SEPARATING AND RECOVERING A DESIRED BIOCHEMICAL PRODUCT FROM A FERMENTATION CULTURE MEDIUM WHEREIN THE PRODUCT IS BEING PRODUCED, WHICH COMPRISES: CONTACTING A TUBULAR, SEMI-PERMEABLE MEMBRANCE MEANS WITH THE CULTURE MEDIUM SO THAT THE PRODUCT DIFFUSES ACROSS THE MEMBRANE INTO AN AQUEOUS MEDIUM FLOWING IN THE MEMBRANE MEANS, EXTRACTING OF THE PRODUCT FROM THE AQUEOUS MEDIUM BY A WATERIMMISCIBLE ORGANIC SOLVENT, ACCUMULATING OF THE EXTRACTED AQUEOUS MEDIUM IN AN AQUEOUS PHASE, WITH THE BIOCHEMICAL PRODUCT REMAINING IN THE ORGANIC SOLVENT PHASE AND WITHDRAWING THE AQUEOUS PHASE FOR PURPOSES OF OPTIONALLY STRIPPING ORGANIC SOLVENT FROM IT, AND RECYCLING IT TO THE TUBULAR, SEMIPERMEABLE MEMBRANE MEANS.
Claim 3
Dependent Claim
25 words
The process according to claim 1 wherein the desired biochemical product is an antibiotic soluble in water, but preferentially soluble in a water-immiscible organic solvent.
Claim 4
Dependent Claim
11 words
The process according to claim 2 wherein the antibiotic is cycloheximide.
Claim 5
Dependent Claim
14 words
The process according to claim 3 wherein the water-immiscible organic solvent is methylene chloride.
Claim 6
Dependent Claim
13 words
The process according to claim 4 wherein the semi-permeable membrane is tubular cellulosic.
Claim 7
Dependent Claim
19 words
The process according to claim 5 wherein the mean pore diameter is in the range of 20A to 50A.
Claim 8
Dependent Claim
24 words
The process according to claim 6 wherein the surface area of semi-permeable membrane is about 500 cm2 per 3 liters volume of fermentation medium.
Claim 9
Dependent Claim
27 words
The process according to claim 7 wherein the volume of aqueous medium is from about 2 percent to about 10 percent of the volume of culture medium.
Claim 10
Dependent Claim
12 words
The process according to claim 8 wherein the volume is 6 percent.
Claim 11
Dependent Claim
128 words
A combination of equipment for producing, separating, accumulating, and recovering a biochemical product which comprises (a) a first vessel containing a volume of a fermentation culture actively producing a desired biochemical product that is preferentially soluble in a water-immiscible organic solvent; (b) a dialysis means consisting of a tubular semi-permeable membrane means in which an aqueous medium is flowing and through which tubular semi-permeable membrane means the biochemical product from said first vessel will diffuse; (c) a second vessel containing a characterizing volume of a water-immiscible organic solvent into which said aqueous medium flowing in said tubular semi-permeable membrane means is discharged, said solvent volume being further characterized by a cross-sectional aqueous phase boundary; and (d) recirculating means connecting said aqueous phase with said tubular semi-permeable membrane means.
Claim 12
Dependent Claim
128 words
A combination of equipment for producing, separating, accumulating, and recovering a biochemical product which comprises (a) a first vessel containing a volume of a fermentation culture actively producing a desired biochemical product that is preferentially soluble in a water-immiscible organic solvent; (b) a dialysis means consisting of a tubular semi-permeable membrane means in which an aqueous medium is flowing and through which tubular semi-permeable membrane means the biochemical product from said first vessel will diffuse; (c) a second vessel containing a characterizing volume of a water-immiscible organic solvent into which said aqueous medium flowing in said tubular semi-permeable membrane means is discharged, said solvent volume being further characterized by a cross-sectional aqueous phase boundary; and (d) recirculating means connecting said aqueous phase with said tubular semi-permeable membrane means.
Claim 13
Dependent Claim
38 words
An apparatus characterized according to claim 10 wherein the tubular, semi-permeable membrane is cellulosic with a mean pore diameter of 20A to 50A and the surface area is about 500 cm2 per 3 liters volume of fermentation culture.
Claim 14
Dependent Claim
19 words
An apparatus characterized according to claim 11 wherein the semi-permeable membrane means is immersed directly in the culture medium.
Claim 15
Dependent Claim
29 words
An apparatus characterized according to claim 12 wherein the aqueous medium is discharged into a second vessel containing a chlorinated hydrocarbon solvent for cycloheximide of greater density than water.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-10-27 | 1979-04-28 | 1979-10-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-10-27 | 1983-04-28 | 1983-10-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-10-27 | 1987-04-28 | 1987-10-28 | $7700.00 | Window Closed / Lapsed |