US3902964A
Expired / Lapsed
US
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Method of and apparatus for chemically separating plasma or serum from formed elements of blood
Assignee: U S MEDICAL RESEARCH AND DEV I
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Filing: 1973-05-21
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Granted: 1975-09-02
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Expires: 1993-05-21
Abstract
Abstract A process for separating plasma or serum from formed elements of the blood by adding a positively charged polymer and a lectin to a tube into which blood is drawn to effect precipitation of the formed elements of the plasma or serum.
Inventors
- 👤 Donald J Greenspan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5002; A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; G01N33/483; G01N33/487; G01N33/49; G01N33/491; B; B01; B01D; B01D21/00; B01D21/01; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
67 words
A METHOD OF SEPARATING SERUM FROM THE FORMED ELEMENTS IN A BLOOD SAMPLE COMPRISING THE STEPS OF: COMBINING A LECTIN HEMAGGLUTININ, A POSITIVELY CHARGE POLYMER AND A BLOOD SAMPLE IN A CONTAINER, AGGLUTINATING THE FORMED ELEMENTS INTO CLUMPS WITH SAID LECTIN AND SAID POSITIVELY CHARGED POLYMER SUCH THAT EACH OF A PLURALITY OF FORMED ELEMENTS IN EACH CLUMP ARE AGGLUTINED WITH ANOTHER FORMED ELEMENT WITH A POLYMERIC
Claim 2
Independent Claim
67 words
A METHOD OF SEPARATING SERUM FROM THE FORMED ELEMENTS IN A BLOOD SAMPLE COMPRISING THE STEPS OF: COMBINING A LECTIN HEMAGGLUTININ, A POSITIVELY CHARGE POLYMER AND A BLOOD SAMPLE IN A CONTAINER, AGGLUTINATING THE FORMED ELEMENTS INTO CLUMPS WITH SAID LECTIN AND SAID POSITIVELY CHARGED POLYMER SUCH THAT EACH OF A PLURALITY OF FORMED ELEMENTS IN EACH CLUMP ARE AGGLUTINED WITH ANOTHER FORMED ELEMENT WITH A POLYMERIC
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein said clumps of formed elements are combined to form a substantially integral mass after precipitating.
Claim 4
Dependent Claim
14 words
The method of claim 2 wherein said clumps drag fibrin downwardly therewith while precipitating.
Claim 5
Independent Claim
158 words
A method of separating formed elements of the blood from serum comprising the steps of: combining a blood sample with a lectin hemagglutinin and a positively charged polymer in a container; agglutinating the formed elements of the blood with said hemagglutinin and said positively charged polymer; and precipitating the agglutinated formed elements of the blood so as to form a layer of serum above 99% of the formed elements of the blood in a given length of time, said layer having a total volume greater then the sum of the volume in a layer of serum above 99% of all elements in a blood sample of the same size combined with said lectin alone after the same given length of time plus the volume in a layer of serum above 99% of all elements of the blood in a blood sample of the same size combined with said positively charged polymer after the same given length of time.
Claim 6
Independent Claim
158 words
A method of separating formed elements of the blood from serum comprising the steps of: combining a blood sample with a lectin hemagglutinin and a positively charged polymer in a container; agglutinating the formed elements of the blood with said hemagglutinin and said positively charged polymer; and precipitating the agglutinated formed elements of the blood so as to form a layer of serum above 99% of the formed elements of the blood in a given length of time, said layer having a total volume greater then the sum of the volume in a layer of serum above 99% of all elements in a blood sample of the same size combined with said lectin alone after the same given length of time plus the volume in a layer of serum above 99% of all elements of the blood in a blood sample of the same size combined with said positively charged polymer after the same given length of time.
Claim 7
Independent Claim
83 words
A method of separating formed elements of the blood from serum comprising the steps of: combining a blood sample with a lectin hemagglutinin and a positively charged polymer; agglutinating the formed elements of the blood with said lectin and said positively charged polymer; and precipitating the agglutinated formed elements of the blood at a rate in excess of the sum of the rates of precipitation achieved with said lectin alone in said blood sample and said positively charged polymer in said blood sample.
Claim 8
Independent Claim
83 words
A method of separating formed elements of the blood from serum comprising the steps of: combining a blood sample with a lectin hemagglutinin and a positively charged polymer; agglutinating the formed elements of the blood with said lectin and said positively charged polymer; and precipitating the agglutinated formed elements of the blood at a rate in excess of the sum of the rates of precipitation achieved with said lectin alone in said blood sample and said positively charged polymer in said blood sample.
Claim 9
Independent Claim
51 words
The method of separating plasma from formed elements of blood comprising the steps of: collecting a sample of blood and placing the sample in a blood collection container; and reacting said blood sample with a lectin hemagglutinin and a positively charged polymer whereby the formed blood elements separate from the plasma.
Claim 10
Independent Claim
51 words
The method of separating plasma from formed elements of blood comprising the steps of: collecting a sample of blood and placing the sample in a blood collection container; and reacting said blood sample with a lectin hemagglutinin and a positively charged polymer whereby the formed blood elements separate from the plasma.
Claim 11
Dependent Claim
13 words
The method according to claim 6 wherein said positively charged polymer is Polybrene.
Claim 12
Dependent Claim
13 words
The method according to claim 6 wherein said hemagglutinin is Bacto Phytohemagglutinin P.
Claim 13
Dependent Claim
19 words
The method of claim 6 wherein the hemagglutinin and the positively charged polymer are simultaneously reacted with the blood.
Claim 14
Dependent Claim
52 words
The method according to claim 6 wherein said blood collection container is hollow cylindrical in configuration and has a longitudinal axis and comprising the additional step of disposing said blood collection container with its longitudinal axis in a substantially horizontal plane to enhance precipitation of the formed blood elements from the plasma.
Claim 15
Dependent Claim
23 words
The method according to claim 6 to produce serum, including the further step of adding a substance for inhibiting the formation of fibrin.
Claim 16
Dependent Claim
11 words
The method according to claim 11 wherein said substance is thrombin.
Claim 17
Dependent Claim
29 words
In combination, a blood collection container containing a hemagglutinin and a posItively charged polymer, said container having volumetric capacity sufficient to hold more than 30 cubic millimeters of blood.
Claim 18
Dependent Claim
29 words
In combination, a blood collection container containing a hemagglutinin and a posItively charged polymer, said container having volumetric capacity sufficient to hold more than 30 cubic millimeters of blood.
Claim 19
Dependent Claim
19 words
The combination according to claim 13 wherein the container also contains a substance for inhibiting the formation of fibrin.
Claim 20
Dependent Claim
16 words
The combination of claim 13 wherein the said container is a vacuum type blood collection tube.
References Cited (Backward Citations)
GB969741A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-01 | 1979-03-03 | 1979-09-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-01 | 1983-03-03 | 1983-09-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-01 | 1987-03-03 | 1987-09-02 | $7700.00 | Window Closed / Lapsed |