US3901782A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the fractionation and identification of proteins by starch gel electrophoresis
Assignee: NOVEX CO Ltd
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Filing: 1974-03-27
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Granted: 1975-08-26
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Expires: 1994-03-27
Abstract
Abstract Protein fractionation by starch gel electrophoresis involving pressure cooking, moulding and cutting the gel; freezing, thawing and homogenizing the sample; fitting a carrier, such as filter paper, containing the sample between pieces of the gel and impressing a D.C. potential through the gel to cause the electrophoretic fractionation.
Inventors
- 👤 Gyorgy Vadasz
- 👤 Mihaly Parkany
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:32
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44704; G01N27/44717
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
158 words
METHOD FOR PROTEIN FRACTIONATION BY STARCH GEL ELECTROPHORESIS, CHARACTERIZED BY COOKING THE STARCH IN A PRESSURE VESSEL AT A PRESSURE OF 1.0 TO 1.3 ATM, REMOVING THE AIR FROM THE STARCH, POURING THE STARCH IN A MOULD INDEPENDENT OF THE ELECTROPHORESIS CELL, CUTTING THE REQUIRED SIZE PART OFF THE GELL BLOCK REMOVED FROM THE MOULD, CUTTING THIS PART IN TWO, PLACING THE HISTOLOGICAL SAMPLE AFTER FEEZING AND THAWING OF THE SAMPLE INTO A HOMOGENIZATION VESSEL, WHEREIN A BEATER IS PLACED, FITTING THIS ASSEMBLY INTO A VIBRATOR, FOLLOWED BY THE HOMOGENIZATION OF THE SAMPLE, FITTING BETWEEN THE GEL PARTS A CARRIER CONTAINING THE SAMPLE, TIGHTLY COMPRESSING THIS MEDIUM WITH THE BLOCK PARTS, DIPPING THE GEL BLOCK THUS PREPARED IN A BUFFER SOLUTION VESSEL CONTAINING A FIRST ELECTRIC CONTACT, FITTING A HYGROSCOPIC BODY IMMERSED SIMILARLY IN A BUFFER CONTAINER EQUIPPED WITH A SECOND ELECTRIC CONTACT, TO THE OTHER END OF THE BLOCK, AND SUPPLYING A D. C. POTENTIAL BETWEEN CONT.
Claim 2
Dependent Claim
158 words
METHOD FOR PROTEIN FRACTIONATION BY STARCH GEL ELECTROPHORESIS, CHARACTERIZED BY COOKING THE STARCH IN A PRESSURE VESSEL AT A PRESSURE OF 1.0 TO 1.3 ATM, REMOVING THE AIR FROM THE STARCH, POURING THE STARCH IN A MOULD INDEPENDENT OF THE ELECTROPHORESIS CELL, CUTTING THE REQUIRED SIZE PART OFF THE GELL BLOCK REMOVED FROM THE MOULD, CUTTING THIS PART IN TWO, PLACING THE HISTOLOGICAL SAMPLE AFTER FEEZING AND THAWING OF THE SAMPLE INTO A HOMOGENIZATION VESSEL, WHEREIN A BEATER IS PLACED, FITTING THIS ASSEMBLY INTO A VIBRATOR, FOLLOWED BY THE HOMOGENIZATION OF THE SAMPLE, FITTING BETWEEN THE GEL PARTS A CARRIER CONTAINING THE SAMPLE, TIGHTLY COMPRESSING THIS MEDIUM WITH THE BLOCK PARTS, DIPPING THE GEL BLOCK THUS PREPARED IN A BUFFER SOLUTION VESSEL CONTAINING A FIRST ELECTRIC CONTACT, FITTING A HYGROSCOPIC BODY IMMERSED SIMILARLY IN A BUFFER CONTAINER EQUIPPED WITH A SECOND ELECTRIC CONTACT, TO THE OTHER END OF THE BLOCK, AND SUPPLYING A D. C. POTENTIAL BETWEEN CONT.
Claim 3
Dependent Claim
41 words
The method of claim 1, characterized by cutting the electrophoretized gel block into slices of a few mm thickness, placing these slices into an incubator solution contained by an incubator vessel made of a material melting at a temperature exceeding 30*C.
Claim 4
Dependent Claim
30 words
The method of claim 1, characterized by fitting the incubated and glycerine treated slices onto a glass plate for preservation, and embedding said treated slices in wax or paraffin there.
Claim 5
Dependent Claim
26 words
The method of claim 1 wherein said freezing and thawing is done in repeated steps, and said carrier is filter paper, porous glass, or ceramic material.
Claim 6
Dependent Claim
28 words
The method of claim 2 wherein said cutting is done with a set of blades matched in a comb-type manner, and said incubator vessel is made of paraffin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-08-25 | 1979-02-24 | 1979-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-08-25 | 1983-02-24 | 1983-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-08-25 | 1987-02-24 | 1987-08-26 | $7700.00 | Window Closed / Lapsed |