US3888629A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Performance of chemical or biological reactions within an absorbent matrix pad
Assignee: Individual
•
Filing: 1972-09-08
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Granted: 1975-06-10
•
Expires: 1992-09-08
Abstract
Abstract A reaction cell for the performance of radioimmunoassay determinations and like saturation analysis reactions has supported within it a matrix pad of absorbent material capable of retaining the necessary reagents for the reaction and serving as a site in which the reaction totally occurs. A separable lower chamber is fitted to the lower end of the cell and contains absorbent material to abut the matrix pad and promote filtration through the pad after the reaction has taken place. An upper reservoir chamber fits to the upper end of the cell to contain liquid for passing through the matrix pad. The matrix pad will commonly contain prior to the reaction a predetermined amount of an antigen or antibody in freeze-dried condition and possibly radioactivity labelled.
Inventors
- 👤 Kenneth Dawson Bagshawe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:55
CPC Classifications
G; G01; G01T; G01T7/00; G01T7/02; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
63 words
A REACTION CELL OF THE TYPE SUITABLE FOR USE IN A RADIOIMMUNASSAY REACTION IN WHICH A LIQUID REACTANT IS EMPLOYED AND A PARTICULATE REACTION PRODUCT RESULTS, SAID CELL COMPRISING: A CONTAINER; MATRIX-PAD MEANS IN SAID CONTAINER FOR HOLDING ALL OF THE LIQUID NECESSARY TO ENABLE SAID REACTION AND RETAINING SAID PARTICULATE REACTION PRODUCT WHILE PERMITTING PASSAGE THERETHROUGH OF OTHER REACTION COMPONENTS, SAID MATRIX
Claim 2
Dependent Claim
63 words
A REACTION CELL OF THE TYPE SUITABLE FOR USE IN A RADIOIMMUNASSAY REACTION IN WHICH A LIQUID REACTANT IS EMPLOYED AND A PARTICULATE REACTION PRODUCT RESULTS, SAID CELL COMPRISING: A CONTAINER; MATRIX-PAD MEANS IN SAID CONTAINER FOR HOLDING ALL OF THE LIQUID NECESSARY TO ENABLE SAID REACTION AND RETAINING SAID PARTICULATE REACTION PRODUCT WHILE PERMITTING PASSAGE THERETHROUGH OF OTHER REACTION COMPONENTS, SAID MATRIX
Claim 3
Dependent Claim
16 words
A cell according to claim 1, wherein the matrix pad occludes the interior of the container.
Claim 4
Dependent Claim
19 words
A cell as claimed in claim 1, wherein the matrix pad contains a predetermined amount of antigen or anitbody.
Claim 5
Dependent Claim
30 words
A cell as claimed in claim 3, wherein the antigen or antibody is in solution and the solution is retained within the matrix pad by surface tension or capillary attraction.
Claim 6
Dependent Claim
14 words
A cell as claimed in claim 3, wherein the antigen or antibody is freeze-dried.
Claim 7
Dependent Claim
38 words
A cell as claimed in claim 5, wherein the matrix pad contains a mixture of a defined amount of corresponding antigen and antibody in a freeze-dried form, at least one of the antibody and antigen being radioactively labelled.
Claim 8
Dependent Claim
25 words
A cell as claimed in claim 1 wherein the matrix pad consists of two or more pad layers in the containeR lying one upon another.
Claim 9
Dependent Claim
44 words
A cell as claimed in claim 7, wherein there are two pad layers one containing a predetermined amount of freeze-dried antibody, and the other containing a predetermined amount of the corresponding freeze-dried antigen, at least one of the antibody and antigen being radioactively labelled.
Claim 10
Dependent Claim
48 words
A cell as claimed in claim 7, wherein there are three pad layers, one containing freeze-dried antibody, a second containing freeze-dried corresponding antigen, and a third containing the said antigen or its corresponding antibody and being radioactively labelled, the said antigen and antibody being present in predetermined amounts.
Claim 11
Dependent Claim
12 words
A cell as claimed in claim 6, wherein the antibody is labelled.
Claim 12
Dependent Claim
14 words
A cell as claimed in claim 6, wherein the radioactive label is radioactive iodine.
Claim 13
Dependent Claim
25 words
A cell as claimed in claim 8, wherein one or more reagent-free matrix pad layers are interposed between the pad layers containing antigen and antibody.
Claim 14
Dependent Claim
38 words
A cell as claimed in claim 1, wherein the matrix pad material is coated, prior to any addition of antigen or antibody, with an inert macromolecular material to inhibit binding of antigen or antibody reactants to the fibres.
Claim 15
Dependent Claim
13 words
A cell as claimed in claim 13, wherein the coating material is albumin.
Claim 16
Dependent Claim
28 words
A cell as claimed in claim 1, wherein a defined amount of a reagent antibody or antigen and an inert macromolecular material are adsorbed on the matrix fibres.
Claim 17
Dependent Claim
16 words
A cell as claimed in claim 15, wherein the antibody is adsorbed on the matrix fibres.
Claim 18
Dependent Claim
27 words
A cell as claimed in claim 1, whereof the lower part communicates with a separable lower chamber suitable for receiving a filtrate passing through the matrix pad.
Claim 19
Dependent Claim
33 words
A cell as claimed in claim 17, wherein the lower chamber contains a porous absorbent material for absorbing the filtrate, the upper portion of which material abuts or nearly abuts the matrix pad.
Claim 20
Dependent Claim
30 words
A cell as claimed in claim 1, whereof the upper part communicates with a separable upper reservoir chamber suitable for receiving liquid to pass into and through the matrix pad.
Claim 21
Dependent Claim
21 words
A cell as claimed in claim 19, wherein the upper chamber bears retaining means for the matrix pad in the container.
Claim 22
Dependent Claim
33 words
A cell as claimed in claim 20, wherein the retaining means comprises three or more prongs projecting downwardly from the inside of the wall of the upper chamber into the matrix pad container.
Claim 23
Dependent Claim
125 words
A kit of apparatus for the performance of radioimmunoassay determinations, comprising at least two reaction cells according to claim 2 in each of which the matrix pad contains a predetermined amount of antibody, for each cell a separate upper liquid reservoir chamber that can be fitted in communication with the upper end of the cell, for each cell a separate lower filtrate chamber that can be fitted in communication with the lower end of the cell and contains absorbent material to abut or substantially abut the matrix pad in the cell to promote filtration, a separate container of the corresponding antigen radioactively labelled, a separate container of a liquid buffer, and at least one separate container of a predetermined amount of the unlabelled corresponding antigen.
Claim 24
Dependent Claim
29 words
A kit according to claim 22, wherein the antigen is digoxin human chorionic somatomammotrophin or insulin or growth hormone each matrix pad containing the antibody to one of these.
Claim 25
Independent Claim
63 words
A method for the performance of a chemical and/or biological reaction, for example saturation analysis employing a radioactive marker, said method comprising the steps of: performing said reaction wholly inside an absorbent matrix pad which is chemically inactive with respect to said reaction; removing reactant matter from said matrix pad; and retaining a particulate reaction product from said reaction in said matrix pad.
Claim 26
Independent Claim
63 words
A method for the performance of a chemical and/or biological reaction, for example saturation analysis employing a radioactive marker, said method comprising the steps of: performing said reaction wholly inside an absorbent matrix pad which is chemically inactive with respect to said reaction; removing reactant matter from said matrix pad; and retaining a particulate reaction product from said reaction in said matrix pad.
Claim 27
Dependent Claim
19 words
A method as claimed in claim 24 in which the reaction takes place exclusively in the absorbent porous matrix.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-09 | 1978-12-09 | 1979-06-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-09 | 1982-12-09 | 1983-06-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-09 | 1986-12-09 | 1987-06-10 | $7700.00 | Window Closed / Lapsed |