US3884579A
Expired / Lapsed
US
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Method for counting blood platelets
Assignee: Cambridge Chemical Products Inc
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Filing: 1973-06-14
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Granted: 1975-05-20
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Expires: 1993-06-14
Abstract
Abstract To facilitate the direct observation and counting of platelets in whole blood serum, a blood sample is mixed with a solution which hemolyzes the red and white cells in a short time without destroying any of the platelets. The platelets can then be observed and counted through a microscope without being masked by the larger cells. The composition includes a dye, preservatives and fixatives and a surfactant which lowers the surface tension of the solution to a controlled level which hemolyzes the red and white cells while leaving the higher surface tension platelets undisturbed.
Inventors
- 👤 Thomas Mauthner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N1/00; G01N1/28; G01N1/30; G01N2001/305; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
53 words
The method of counting platelets in a specimen of whole blood serum comprising dissolving the specimen in a solution having a surface tension in distilled water, measured at 22*C of between 29.8 and 31.0 dynes/cm, and observing and counting the platelets through a microscope after the red and white cells have been hemolyzed.
Claim 2
Independent Claim
53 words
The method of counting platelets in a specimen of whole blood serum comprising dissolving the specimen in a solution having a surface tension in distilled water, measured at 22*C of between 29.8 and 31.0 dynes/cm, and observing and counting the platelets through a microscope after the red and white cells have been hemolyzed.
Claim 3
Independent Claim
61 words
The method of counting the blood platelets in a sample of whole blood serum comprising dissolving the sample in a solution containing a surfactant, the solution having a surface tension in distilled water, measured at 22* C. of between 29.8 and 31.0 dynes/cm. to hemolyze the red and white cells, and observing and counting the platelets as viewed with a microscope.
Claim 4
Independent Claim
61 words
The method of counting the blood platelets in a sample of whole blood serum comprising dissolving the sample in a solution containing a surfactant, the solution having a surface tension in distilled water, measured at 22* C. of between 29.8 and 31.0 dynes/cm. to hemolyze the red and white cells, and observing and counting the platelets as viewed with a microscope.
References Cited (Backward Citations)
US3503684A
Cited
US3733548A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-05-19 | 1978-11-18 | 1979-05-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-05-19 | 1982-11-18 | 1983-05-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-05-19 | 1986-11-18 | 1987-05-20 | $7700.00 | Window Closed / Lapsed |