US3879280A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Gel slab electrophoresis cell and electrophoresis apparatus utilizing same
Assignee: United States Department of Health and Human Services
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Filing: 1974-04-16
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Granted: 1975-04-22
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Expires: 1994-04-16
Abstract
Abstract An electrophoresis cell for use in the simultaneous electrophoresis of multiple samples in an elongated gel slab supporting medium in a multiphasic buffer system. The cell is provided with permanent sample partitions which define a plurality of individual sample-receiving compartments within the upper portion of the cell, enabling the cell to be used with mechanically labile stacking gels. The gel slab is prepared in situ within the cell, after which the samples are introduced into the partitioned compartments and electrophoresis carried out.
Inventors
- 👤 John I Peterson
- 👤 Andreas Chrambach
- 👤 Jr Julian W Holland
- 👤 William R Dehn
- 👤 H Douglas Swank
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:55
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44782
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
209 words
A gel slab electrophoresis cell adapted for use in an apparatus for simultaneously electrophoretically separating the ionic components of a plurality of samples, said cell comprising a rear panel defining the rear wall of said cell, a pair of spacer means fixed to the opposite sides of the front face of said rear panel and extending along the entire length thereof thereby defining the side walls of said cell, an upper front panel fixed to said pair of spacer means and extending from the top of said cell partially along the length thereof, a lower front panel removably secured to said pair of spacer means and extending from the bottom of said upper front panel to the bottom of said cell, said upper front panel and said lower front panel thereby together defining the front wall of said cell, said rear, side and front walls thereby defining between them an open-ended elongated space for receiving a gel, and partition means fixed to said rear panel and said upper front panel between said spacer means and extending from the top of said cell to a point above the bottom of said upper front panel to thereby define a plurality of individual sample-receiving compartments within the upper portion of said cell.
Claim 2
Dependent Claim
209 words
A gel slab electrophoresis cell adapted for use in an apparatus for simultaneously electrophoretically separating the ionic components of a plurality of samples, said cell comprising a rear panel defining the rear wall of said cell, a pair of spacer means fixed to the opposite sides of the front face of said rear panel and extending along the entire length thereof thereby defining the side walls of said cell, an upper front panel fixed to said pair of spacer means and extending from the top of said cell partially along the length thereof, a lower front panel removably secured to said pair of spacer means and extending from the bottom of said upper front panel to the bottom of said cell, said upper front panel and said lower front panel thereby together defining the front wall of said cell, said rear, side and front walls thereby defining between them an open-ended elongated space for receiving a gel, and partition means fixed to said rear panel and said upper front panel between said spacer means and extending from the top of said cell to a point above the bottom of said upper front panel to thereby define a plurality of individual sample-receiving compartments within the upper portion of said cell.
Claim 3
Dependent Claim
209 words
A gel slab electrophoresis cell adapted for use in an apparatus for simultaneously electrophoretically separating the ionic components of a plurality of samples, said cell comprising a rear panel defining the rear wall of said cell, a pair of spacer means fixed to the opposite sides of the front face of said rear panel and extending along the entire length thereof thereby defining the side walls of said cell, an upper front panel fixed to said pair of spacer means and extending from the top of said cell partially along the length thereof, a lower front panel removably secured to said pair of spacer means and extending from the bottom of said upper front panel to the bottom of said cell, said upper front panel and said lower front panel thereby together defining the front wall of said cell, said rear, side and front walls thereby defining between them an open-ended elongated space for receiving a gel, and partition means fixed to said rear panel and said upper front panel between said spacer means and extending from the top of said cell to a point above the bottom of said upper front panel to thereby define a plurality of individual sample-receiving compartments within the upper portion of said cell.
Claim 4
Dependent Claim
209 words
A gel slab electrophoresis cell adapted for use in an apparatus for simultaneously electrophoretically separating the ionic components of a plurality of samples, said cell comprising a rear panel defining the rear wall of said cell, a pair of spacer means fixed to the opposite sides of the front face of said rear panel and extending along the entire length thereof thereby defining the side walls of said cell, an upper front panel fixed to said pair of spacer means and extending from the top of said cell partially along the length thereof, a lower front panel removably secured to said pair of spacer means and extending from the bottom of said upper front panel to the bottom of said cell, said upper front panel and said lower front panel thereby together defining the front wall of said cell, said rear, side and front walls thereby defining between them an open-ended elongated space for receiving a gel, and partition means fixed to said rear panel and said upper front panel between said spacer means and extending from the top of said cell to a point above the bottom of said upper front panel to thereby define a plurality of individual sample-receiving compartments within the upper portion of said cell.
Claim 5
Dependent Claim
26 words
The gel slab electrophoresis cell of claim 1 wherein said partition means comprises a plurality of equally-spaced partition members defining a plurality of equallydimensioned sample-receiving compartments.
Claim 6
Dependent Claim
34 words
The gel slab electrophoresis cell of claim 2 wherein said rear panel, said pair of spacer means, said upper front panel, said lower front panel and said partition members are all formed of glass.
Claim 7
Dependent Claim
29 words
The gel slab electrophoresis cell of claim 3 wherein said rear panel, said pair of spacer means, said upper front panel and said partition members are fusion bonded together.
Claim 8
Independent Claim
311 words
An apparatus for simultaneously electrophoretically separating the ionic components of a plurality of samples comprising a first container for holding buffer solution and having an elongated opening in its bottom end; a gel slab electrophoresis cell fixed to said bottom end of said first container and having an open upper end in sealing engagement with said opening in the bottom of said first container so as to be in contact with said buffer solution contained therein, said cell comprising a rear panel defining the rear wall of said cell, a pair of spacer means fixed to the opposite sides of the front face of said rear panel and extending along the entire length thereof thereby defining the side walls of said cell, an upper front panel fixed to said pair of spacer means and extending from the top of said cell partially along the length thereof, a lower front panel removably secured to said pair of spacer means and extending from the bottom of said upper front panel to the bottom of said cell, said upper front panel and said lower front panel thereby together defining the front wall of said cell, said rear, side and front walls thereby defining between them an open-ended elongated space for receiving a gel, and partition means fixed to said rear panel and said upper front panel between said spacer means and extending from the top of said cell to a point above the bottom of said upper front panel to thereby define a plurality of individual sample-receiving compartments within the upper portion of said cell; a second container for holding buffer solution disposed perimetrically about said Cell so that the buffer solution is in contact with the open lower end of said cell; and electrode means for maintaining a potential difference between said upper and lower ends of said cell through the buffer solutions.
Claim 9
Independent Claim
311 words
An apparatus for simultaneously electrophoretically separating the ionic components of a plurality of samples comprising a first container for holding buffer solution and having an elongated opening in its bottom end; a gel slab electrophoresis cell fixed to said bottom end of said first container and having an open upper end in sealing engagement with said opening in the bottom of said first container so as to be in contact with said buffer solution contained therein, said cell comprising a rear panel defining the rear wall of said cell, a pair of spacer means fixed to the opposite sides of the front face of said rear panel and extending along the entire length thereof thereby defining the side walls of said cell, an upper front panel fixed to said pair of spacer means and extending from the top of said cell partially along the length thereof, a lower front panel removably secured to said pair of spacer means and extending from the bottom of said upper front panel to the bottom of said cell, said upper front panel and said lower front panel thereby together defining the front wall of said cell, said rear, side and front walls thereby defining between them an open-ended elongated space for receiving a gel, and partition means fixed to said rear panel and said upper front panel between said spacer means and extending from the top of said cell to a point above the bottom of said upper front panel to thereby define a plurality of individual sample-receiving compartments within the upper portion of said cell; a second container for holding buffer solution disposed perimetrically about said Cell so that the buffer solution is in contact with the open lower end of said cell; and electrode means for maintaining a potential difference between said upper and lower ends of said cell through the buffer solutions.
Claim 10
Dependent Claim
23 words
The apparatus of claim 5 wherein said partition means comprises a plurality of equally-spaced partition members defining a plurality of equally-dimensioned sample-receiving compartments.
Claim 11
Dependent Claim
24 words
The apparatus of claim 5 wherein said first container rests on top of said second container with said cell extending into said second container.
Claim 12
Dependent Claim
15 words
The apparatus of claim 5 wherein said second container is provided with a temperature-controlling jacket.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-21 | 1978-10-21 | 1979-04-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-21 | 1982-10-21 | 1983-04-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-21 | 1986-10-21 | 1987-04-22 | $7700.00 | Window Closed / Lapsed |