US3876808A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of a balanced mixed viable bacterial culture
Assignee: Wenner-Gren Medical Lab AB
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Filing: 1973-06-11
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Granted: 1975-04-08
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Expires: 1993-06-11
Abstract
Abstract A dry mixed culture containing Lactobacillus bulgaricus and Streptococcus thermophilus in the ratio of 40:60 to 60:40 percent is produced by using for cultivation a skimmilk substrate containing cyanocobalamine, or cyanocobalamine and lysin and/or cystin, and drying after cultivation.
Inventors
- 👤 Leo Anderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:35
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; A; A23; A23C; A23C9/00; A23C9/12; A23C9/123; A23C9/1232; A23C9/1238; C12N1/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/853; Y10S435/885
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
100 words
A PROCESS FOR PRODUCING A DRY COMPOSITION COMPRISING SKIM MILK AND A BALANCED, VIABLE, AND LIVING CULTURE OF LACTOBACILLUS BULGARICUS AND STREPTOCCOCCUS THERMOPHILUS, SAID METHOD COMPRISING THE STEPS OF: PROVIDING A CONDENSED SKIM MILK NUTRIENT SUBSTRATE HAVING A SOLIDS CONTENT OF FROM 18 TO 24 PERCENT, ADDING CYANOCOBALAMINE TO SAID NUTRIENT SUBSTRATE IN THE AMOUNT OF FROM 0.5 TO 5 MILIGRAMS PER 100 LITERS OF NUTRIENT SUBSTRATE, CULTIVATING THE BACTERIA ON SAID SUBSTRATE UNTIL LACTROBACILLUS BULGARICUS AND STREPTOCCOCCUS THEREMOPHILUS ARE OBTAINED IN A RATIO OF FROM 10:60 TO 60:40 PERCENT, AND DRYING THE NUTRIENT SUBSTRATE AND THE BACTERIA CULTIVATED THEREON.
Claim 2
Dependent Claim
100 words
A PROCESS FOR PRODUCING A DRY COMPOSITION COMPRISING SKIM MILK AND A BALANCED, VIABLE, AND LIVING CULTURE OF LACTOBACILLUS BULGARICUS AND STREPTOCCOCCUS THERMOPHILUS, SAID METHOD COMPRISING THE STEPS OF: PROVIDING A CONDENSED SKIM MILK NUTRIENT SUBSTRATE HAVING A SOLIDS CONTENT OF FROM 18 TO 24 PERCENT, ADDING CYANOCOBALAMINE TO SAID NUTRIENT SUBSTRATE IN THE AMOUNT OF FROM 0.5 TO 5 MILIGRAMS PER 100 LITERS OF NUTRIENT SUBSTRATE, CULTIVATING THE BACTERIA ON SAID SUBSTRATE UNTIL LACTROBACILLUS BULGARICUS AND STREPTOCCOCCUS THEREMOPHILUS ARE OBTAINED IN A RATIO OF FROM 10:60 TO 60:40 PERCENT, AND DRYING THE NUTRIENT SUBSTRATE AND THE BACTERIA CULTIVATED THEREON.
Claim 3
Dependent Claim
48 words
The process of claim 1, together with the step of adding to the nutrient substrate before the bacteria are cultivated thereon, one or both of the following: from 50 to 500 grams of lysin and from 10 to 1000 grams of cystin per 100 liters of nutrient substrate.
Claim 4
Dependent Claim
24 words
The process of claim 1, wherein the nutrient substrate and the bacteria cultivated thereon are dried at a temperature of from 70.degree. to 85.degree.C.
Claim 5
Dependent Claim
13 words
The process of claim 3, wherein the temperature is from 75.degree. to 80.degree.C.
References Cited (Backward Citations)
US3320130A
Cited
US3420742A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-07 | 1978-10-07 | 1979-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-07 | 1982-10-07 | 1983-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-07 | 1986-10-07 | 1987-04-08 | $7700.00 | Window Closed / Lapsed |