US3874384A
Expired / Lapsed
US
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Improved blood storage unit and method of storing blood
Assignee: Kendall Mcgraw Laboratories Inc
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Filing: 1973-03-29
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Granted: 1975-04-01
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Expires: 1993-03-29
Abstract
Abstract Blood storage units comprising containers with aqueous preservative solutions therein are improved by incorporating dihydroxyacetone (DHA) together with L-ascorbate (vitamin C) in the preservative solutions. The invention also relates to a method of storing human blood wherein viable red cells are stored in contact with both DHA and L-ascorbate. The preservative solution of the blood storage unit may also contain an anticoagulant such as citrate, a sugar energy source such as dextrose, and an ATP maintaining agent such as adenine. Where the blood storage unit is to be heat sterilized, as preferred, a blood bag providing a separate compartment for part of the preservative agents prevents deterioration of the preservative agents, the DHA and sugar energy source being heat sterilized separately for later admixture with the ascorbate, citrate anticoagulant and adenine. Separate pH control can also thereby be provided for the heat sterilization.
Inventors
- 👤 Fred H Deindoerfer
- 👤 Jon M Brake
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:02
CPC Classifications
A; A61; A61J; A61J1/00; A61J1/05; A61J1/10; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A01N1/14; A01N1/146; A61J1/12
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
95 words
A BLOOD STORAGE UNIT COMPRISING A CONTAINER FOR RECEIVING AND STORING A PREDETERMINED VOLUME OF BLOOD AND PRESERVATIVE SOLUTION ADMIXABLE WITH THE BLOOD STORED IN SAID CONTAINER, SAID PRESERVATIVE SOLUTION BEING STERILE AND PROVIDING A SUGAR ENERGY SOURCE AND AN ANTI-COAGULANT FOR PRESERVING SAID BLOOD, SAID PRESERVATIVE SOLUTION ALSO PROVIDING FOR COOPERATIVE ADMIXTURE WITH SAID STORED BLOOD AN AMOUNT OF DIHYDROXYACETONE (DHA) EQUAL TO 5 TO 100 MILLIMOLES (MM) PER LITER OF SAID PREDETERMINED BLOOD VOLUME TOGETHER WITH AN AMOUNT OF L-ASCORBATE EQUAL TO 0.5 TO 20 MM PER LITER OF SAID PREDETERMINED BLOOD VOLUME.
Claim 2
Dependent Claim
95 words
A BLOOD STORAGE UNIT COMPRISING A CONTAINER FOR RECEIVING AND STORING A PREDETERMINED VOLUME OF BLOOD AND PRESERVATIVE SOLUTION ADMIXABLE WITH THE BLOOD STORED IN SAID CONTAINER, SAID PRESERVATIVE SOLUTION BEING STERILE AND PROVIDING A SUGAR ENERGY SOURCE AND AN ANTI-COAGULANT FOR PRESERVING SAID BLOOD, SAID PRESERVATIVE SOLUTION ALSO PROVIDING FOR COOPERATIVE ADMIXTURE WITH SAID STORED BLOOD AN AMOUNT OF DIHYDROXYACETONE (DHA) EQUAL TO 5 TO 100 MILLIMOLES (MM) PER LITER OF SAID PREDETERMINED BLOOD VOLUME TOGETHER WITH AN AMOUNT OF L-ASCORBATE EQUAL TO 0.5 TO 20 MM PER LITER OF SAID PREDETERMINED BLOOD VOLUME.
Claim 3
Dependent Claim
30 words
The blood storage unit of claim 1 wherein said DHA is present in an amount of from 10 to 30 mM of DHA per liter of said predetermined blood volume.
Claim 4
Dependent Claim
31 words
The blood storage unit of claim 1 wherein said preservative solution also provides adenine in an amount equal to from 0.1 to 1.0 mM per liter of said predetermined blood volume.
Claim 5
Dependent Claim
27 words
The blood storage unit of claim 1 wherein said preservative solution provides from 1 to 10 mM of said L-ascorbate per liter of said predetermined blood volume.
Claim 6
Independent Claim
86 words
The method of maintaining the 2,3-diphosphoglycerate (2,3-DPG) content of viable red cells of whole human blood, comprising incorporating in said whole blood from 5 to 100 millimoles (mM) of dihydroxyacetone (DHA) per liter of said blood together with 0.5 to 20 mM of L-ascorbate per liter of said blood, and holding said blood with said red cells in contact with said DHA and L-ascorbate for sufficient time to maintain their 2,3-DPG content at a level resulting from the synergistic action of said DHA and said L-ascorbate.
Claim 7
Independent Claim
86 words
The method of maintaining the 2,3-diphosphoglycerate (2,3-DPG) content of viable red cells of whole human blood, comprising incorporating in said whole blood from 5 to 100 millimoles (mM) of dihydroxyacetone (DHA) per liter of said blood together with 0.5 to 20 mM of L-ascorbate per liter of said blood, and holding said blood with said red cells in contact with said DHA and L-ascorbate for sufficient time to maintain their 2,3-DPG content at a level resulting from the synergistic action of said DHA and said L-ascorbate.
Claim 8
Dependent Claim
35 words
The method of claim 5 wherein said DHA and said L-ascorbate are incorporated in said blood in amounts of from 15 to 45 mM DHA and 1.5 to 15 mM ascorbate per liter of blood.
Claim 9
Independent Claim
61 words
The method of maintaining 2,3-diphosphoglycerate (2,3-DPG) content of the red cells of whole human blood under storage condiTions, comprising adding to said whole blood from 5 to 100 millimoles (mM) of dihydroxyacetone (DHA) and from 0.5 to 20 mM of L-ascorbate per liter of blood, and storing said DHA and ascorbate containing blood without freezing at a temperature below 10* C.
Claim 10
Independent Claim
61 words
The method of maintaining 2,3-diphosphoglycerate (2,3-DPG) content of the red cells of whole human blood under storage condiTions, comprising adding to said whole blood from 5 to 100 millimoles (mM) of dihydroxyacetone (DHA) and from 0.5 to 20 mM of L-ascorbate per liter of blood, and storing said DHA and ascorbate containing blood without freezing at a temperature below 10* C.
Claim 11
Dependent Claim
33 words
The method of claim 7 wherein from 10 to 30 mM of said DHA and from 1 to 10 mM of said L-ascorbate are added to said blood immediately after the collection thereof.
Claim 12
Dependent Claim
20 words
The method of claim 7 in which said blood is stored for a period of from 3 to 6 weeks.
Claim 13
Dependent Claim
38 words
A PRESERVATIVE SOLUTION FOR ADDITION TO STORED BLOOD, COMPRISING A STERILE AQUEOUS SOLUTION OF DIHYDROXYACETONE (DHA) AND L-ASCORBATE, SAID SOLUTION CONTAINING FROM 0.5 TO 20 MM OF SAID L-ASCORBATE PER EACH 5 TO 100 MM OF SAID DHA.
Claim 14
Dependent Claim
38 words
A PRESERVATIVE SOLUTION FOR ADDITION TO STORED BLOOD, COMPRISING A STERILE AQUEOUS SOLUTION OF DIHYDROXYACETONE (DHA) AND L-ASCORBATE, SAID SOLUTION CONTAINING FROM 0.5 TO 20 MM OF SAID L-ASCORBATE PER EACH 5 TO 100 MM OF SAID DHA.
Claim 15
Dependent Claim
32 words
A preservative solution for addition to substantially 0.5 liters of whole blood, comprising a sterile aqueous solution containing from 2.5 to 50 mM dihydroxyacetone together with 0.25 to 10 mM of L-ascrobate.
Claim 16
Dependent Claim
32 words
A preservative solution for addition to substantially 0.5 liters of whole blood, comprising a sterile aqueous solution containing from 2.5 to 50 mM dihydroxyacetone together with 0.25 to 10 mM of L-ascrobate.
Claim 17
Dependent Claim
152 words
A HEAT-STERILIZED BLOOD STORAGE UNIT, COMPRISING A CONTAINER FOR RECEIVING AND STORING A PREDETERMINED VOLUME OF BLOOD, A FIRST STERILE AQUEOUS PRESERVATIVE SOLUTION IN SAID CONTAINER, MEANS PROVIDING A SEPARATE COMPARTMENT, A SECOND STERILE AQUEOUS PRESERVATIVE SOLUTION IN SAID COMPARTMENT, MEANS PERMITTING SAID SECOND SOLUTION TO BE INTRODUCED INTO SAID CONTAINER FOR ADMIXTURE WITH SAID FIRST SOLUTION AND WITH SAID PREDETERMINED VOLUME OF BLOOD, SAID FIRST AND SECOND SOLUTIONS TOGETHER PROVIDING A SUGAR ENERGY SOURCE, AN ANTICOAGULANT, AN AMOUNT OF DIHYDROXYACETONE (DHA) EQUAL TO 5 TO 100 MILLIMOLES (MM) PER LITER OF SAID PREDETERMINED BLOOD VOLUME, AND AN AMOUNT OF L-ASCORBATE EQUAL TO 0.5 TO 20 MM PER LITER OF SAID PREDETERMINED BLOOD VOLUME, SAID L-ASCORBATE BEING CONTAINED ONLY IN ONE OF SAID FIRST AND SECOND SOLUTIONS PRIOR TO SAID ADMIXTURE THEREOF, AND SAID SUGAR ENERGY SOURCE AND SAID DHA BEING CONTAINED ONLY IN THE OTHER OF SAID FIRST AND SECOND SOLUTIONS.
Claim 18
Dependent Claim
152 words
A HEAT-STERILIZED BLOOD STORAGE UNIT, COMPRISING A CONTAINER FOR RECEIVING AND STORING A PREDETERMINED VOLUME OF BLOOD, A FIRST STERILE AQUEOUS PRESERVATIVE SOLUTION IN SAID CONTAINER, MEANS PROVIDING A SEPARATE COMPARTMENT, A SECOND STERILE AQUEOUS PRESERVATIVE SOLUTION IN SAID COMPARTMENT, MEANS PERMITTING SAID SECOND SOLUTION TO BE INTRODUCED INTO SAID CONTAINER FOR ADMIXTURE WITH SAID FIRST SOLUTION AND WITH SAID PREDETERMINED VOLUME OF BLOOD, SAID FIRST AND SECOND SOLUTIONS TOGETHER PROVIDING A SUGAR ENERGY SOURCE, AN ANTICOAGULANT, AN AMOUNT OF DIHYDROXYACETONE (DHA) EQUAL TO 5 TO 100 MILLIMOLES (MM) PER LITER OF SAID PREDETERMINED BLOOD VOLUME, AND AN AMOUNT OF L-ASCORBATE EQUAL TO 0.5 TO 20 MM PER LITER OF SAID PREDETERMINED BLOOD VOLUME, SAID L-ASCORBATE BEING CONTAINED ONLY IN ONE OF SAID FIRST AND SECOND SOLUTIONS PRIOR TO SAID ADMIXTURE THEREOF, AND SAID SUGAR ENERGY SOURCE AND SAID DHA BEING CONTAINED ONLY IN THE OTHER OF SAID FIRST AND SECOND SOLUTIONS.
Claim 19
Dependent Claim
30 words
The blood storage unit of claim 12 wherein said DHA is present in an amount of from 10 to 30 mM of DHA per liter of said predetermined blood volume.
Claim 20
Dependent Claim
33 words
The blood storage unit of claim 12 wherein one of said preservative solutions also contains adenine in an amount equal to from 0.1 to 1.0 mM per liter of said predetermined blood volume.
Claim 21
Dependent Claim
29 words
The improved blood storage unit of claim 13 wherein said one preservative solution contains from 1 to 10 mM of said L-ascorbate per liter of said predetermined blood volume.
References Cited (Backward Citations)
US3703438A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-31 | 1978-09-30 | 1979-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-31 | 1982-09-30 | 1983-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-31 | 1986-09-30 | 1987-04-01 | $7700.00 | Window Closed / Lapsed |