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US3873684A
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Sensitized cell for use in the passive agglutination and method of preparing the same
Assignee: Fujirebio Inc
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Filing: 1973-12-19
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Granted: 1975-03-25
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Expires: 1993-12-19
Abstract
Abstract This invention discloses sensitized cells for use in passive agglutination. Such cells are prepared by using as a carrier micro-organisms which have been subjected to the cell wall fixation thereof with formaldehyde and then the intracellular fixation thereof at a temperature above 90*C for at least 5 minutes and adsorbing an antigen or an antibody on the carrier.
Inventors
- 👤 Koichiro Fujita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:49
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
100 words
A METHOD OF PREPARING A SENSITIZED CELL FOR USE IN PASSIVE AGGLUTINATION COMPRISING SUBJECTING A MICROOGRANSIUM TO CELL WALL FIXATION BY TREATMENT WITH FORMALDEHYDE AT A CONCENTRATION OF FROM 0.2 TO 20% AT A TEMPERATURE RANGING BETWEEN 0* AND 37*C FOR A PERIOD OF TIME VARYING UP TO ABOUT 12 HOURS FOLLOWED BY HEATING THE FORMALDEHYDE TREATED ORGANISM AT AN ELEVATED TEMPERATURE VARYING BETWEEN 90* AND 135*C FOR A A PERIOD OF TIME RANGING FROM 5 MINUTES TO 5 HOURS, TREATING THE CELL WITH TANNIC ACID AND ADSORBING ON THE SURFACE OF SAID TANNIC ACID-TREATED MICROORGANISM, AN ANTIGEN OR ANTIBODY.
Claim 2
Independent Claim
100 words
A METHOD OF PREPARING A SENSITIZED CELL FOR USE IN PASSIVE AGGLUTINATION COMPRISING SUBJECTING A MICROOGRANSIUM TO CELL WALL FIXATION BY TREATMENT WITH FORMALDEHYDE AT A CONCENTRATION OF FROM 0.2 TO 20% AT A TEMPERATURE RANGING BETWEEN 0* AND 37*C FOR A PERIOD OF TIME VARYING UP TO ABOUT 12 HOURS FOLLOWED BY HEATING THE FORMALDEHYDE TREATED ORGANISM AT AN ELEVATED TEMPERATURE VARYING BETWEEN 90* AND 135*C FOR A A PERIOD OF TIME RANGING FROM 5 MINUTES TO 5 HOURS, TREATING THE CELL WITH TANNIC ACID AND ADSORBING ON THE SURFACE OF SAID TANNIC ACID-TREATED MICROORGANISM, AN ANTIGEN OR ANTIBODY.
Claim 3
Dependent Claim
10 words
The sensitized cell prepared by the method of claim 1.
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein the intracellular fixation of the microorganism is carried out in an aqueous solution.
Claim 5
Dependent Claim
16 words
The method of claim 1 wherein the microorganism varies in size between 0.2 and 5 microns.
Claim 6
Dependent Claim
14 words
The method of claim 1 wherein the microorganism is Brucella abortus or Saccharomyces cerevisiae.
Claim 7
Dependent Claim
56 words
The method of claim 1 wherein the microorganism is selected from the group consisting of Saccharomyces cerevisiae, SerratIa marcescens, Brucella abortus, Bacillus subtilis, Bacillus cereus, Bacillus megatherium, Proteus vulgaris, Bacterium zopfii, Lactobacillus bulgaricus, Acetobactor suboxydans, Streptococcus lactis, Streptococcus cremoris, Tetracoccus liquefaciens, Sarcina lutea, Micrococcus lysodelkties, Straphylococcus, Saccharomyces delbruckii lindner, Eremascus fertilis, Endomyces bisporus and Cryptococcus albidus.
Claim 8
Dependent Claim
10 words
The sensitized cell prepared by the method of claim 6.
References Cited (Backward Citations)
US3553310A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |