Abstract A method and apparatus for deriving an oxygen association rate curve for a blood sample wherein the sample is introduced into a transparent-walled cell and is deoxygenated by contact with a deoxygenated fluorocarbon material. The cell is located in the optical path of two time-shared respective monochromatic beams, one having a wavelength at which there is substantially no change in absorbance as between oxygenated and deoxygenated blood and the other having a wavelength at which there is a relatively large change in absorbance as between oxygenated and deoxygenated blood. The difference in absorbance through the cell of the two wavelengths is measured and recorded while oxygenated fluorocarbon titrant material is pumped through the cell, the cell having a restricted flow passage for the titrant material which acts to separate the blood therefrom and retain it in the cell while the titration is taking place.
A METHOD OF DERIVING AN OXYGEN ASSOCIATION RATE CURVE FOR A BLOOD SAMPLE COMPRISING DEOXYGENATING THE SAMPLE, INTRODUCING AN OXYGENATING AGENT INTO THE SAMPLE, PASSING INTE RESPECTIVE MONOCHROMATIC WAVELENGHTS THROUGH THE SAMPLE WHILE IT IS BEING OXYGENSTED, ONE WAVELENGTH AT WHICH THERE IS SUBSTANTIALLY NO CHANGE IN ABSORBANCE AS BETWEEN OXYGENATED AND DEOXYGENATED BLOOD AND THE OTHER WAVELENGTH AT WHICH THERE IS RELATIVELY LARGE CHANGE IN ABSORBANCE AS BETWEEN OXYGENATED AND DEOXYGENATED BLOOD, AND PLOTTING THE DIFFERENCE IN ABSORBANCE FOR THE WAVELENGTHS WITH TIME AS THE SAMPLE IS BEING OXYGENATED.
Claim 2
Independent Claim
90 words
A METHOD OF DERIVING AN OXYGEN ASSOCIATION RATE CURVE FOR A BLOOD SAMPLE COMPRISING DEOXYGENATING THE SAMPLE, INTRODUCING AN OXYGENATING AGENT INTO THE SAMPLE, PASSING INTE RESPECTIVE MONOCHROMATIC WAVELENGHTS THROUGH THE SAMPLE WHILE IT IS BEING OXYGENSTED, ONE WAVELENGTH AT WHICH THERE IS SUBSTANTIALLY NO CHANGE IN ABSORBANCE AS BETWEEN OXYGENATED AND DEOXYGENATED BLOOD AND THE OTHER WAVELENGTH AT WHICH THERE IS RELATIVELY LARGE CHANGE IN ABSORBANCE AS BETWEEN OXYGENATED AND DEOXYGENATED BLOOD, AND PLOTTING THE DIFFERENCE IN ABSORBANCE FOR THE WAVELENGTHS WITH TIME AS THE SAMPLE IS BEING OXYGENATED.
Claim 3
Dependent Claim
25 words
The method of claim 1, and wherein the blood sample and the oxygenating agent are agitated while oxygenation of the blood sample is taking place.
Claim 4
Dependent Claim
28 words
The method of claim 1, and wherein said one wavelength has a value of approximately 448 nm and said other wavelength has a value of approximately 439 nm.
Claim 5
Dependent Claim
15 words
The method of claim 1, and wherein the oxygenating agent comprises oxygenated fluorocarbon liquid material.
Claim 6
Dependent Claim
19 words
The method of claim 4, and wherein deoxygenation of the sample comprises exposing it to deoxygenated fluorocarbon liquid material.