US3854470A
Expired / Lapsed
US
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Reproduction processes for cellular bodies
Assignee: Individual
•
Filing: 1973-11-23
•
Granted: 1974-12-17
•
Expires: 1993-11-23
Abstract
Abstract Disclosed a process for making greater use of female gametes in herbivorous mannals. The techniques include methods of obtaining ova from donor, preparation of recipients, detection of oestrus, and transplant techniques. Also disclosed are methods of making greater desired use of ova of these mammals. Among those are techniques by sex determination, for freezing and thawing eggs, for tissue culture of eggs and for clonal production of like embroyos utilized for transplantation to achieve a greater number of clonal differentiated cellular bodies having like genetic characteristics.
Inventors
- 👤 L Augspurger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:50
CPC Classifications
A; A61; A61D; A61D7/00; A61D19/00; A61D19/04; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/54
Claims Analysis
Total Claims: 112
Claim 1
Independent Claim
154 words
A process for embyro transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor by administration of gonadotrophin, receiving the ova from the donor, placing the recovered ova in tirrus culture medium and storing the recovered ova for a period of time, preparing recipients of prospective transplantations and determining the time of ovulation in the recipients, selecting one or more of the individual ovum or cellular bodies from said stored ova in said culture medium, transplanting selected individual ovum or cellular bodies derived from said donor to a selected recipient at a time corresponding to a synchronization of the individual ovum or cellular bodies transplanted and the time of normal fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst had the recipient been fertilized.
Claim 2
Dependent Claim
154 words
A process for embyro transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor by administration of gonadotrophin, receiving the ova from the donor, placing the recovered ova in tirrus culture medium and storing the recovered ova for a period of time, preparing recipients of prospective transplantations and determining the time of ovulation in the recipients, selecting one or more of the individual ovum or cellular bodies from said stored ova in said culture medium, transplanting selected individual ovum or cellular bodies derived from said donor to a selected recipient at a time corresponding to a synchronization of the individual ovum or cellular bodies transplanted and the time of normal fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst had the recipient been fertilized.
Claim 3
Dependent Claim
17 words
A process according to claim 1 wherein the ova are frozen with tissue culture medium before transplantation.
Claim 4
Dependent Claim
29 words
A process according to claim 1 wherein the ova recovered from the donor have cells seperated from individual ovum and the development to the blastocyst stage is thus delayed.
Claim 5
Dependent Claim
16 words
A process according to claim 1 wherein transplantation is to the uterine horn of the recipient.
Claim 6
Dependent Claim
15 words
A process according to claim 1 wherein transplantation is to the oviduct of the recipient.
Claim 7
Dependent Claim
28 words
A process according to claim 1 wherein transplantation is to the oviduct of the recipient via the uterine horn and via a cannula inserted through the isthmic junction.
Claim 8
Dependent Claim
39 words
A process according to claim 1 wherein the ovum is permitted differentiation into a plurality of cellular bodies no further developed than the blastocyst stage, and one or more differentiated cells are recovered for microscopic examination to determine sex.
Claim 9
Dependent Claim
21 words
A process according to claim 1 wherein the ova donor is administered luteinizing hormone 4 to 6 days after gonodotrophin administration.
Claim 10
Dependent Claim
21 words
A process according to claim 1 wherein the donor is fed a high energy ration during the period of follicular development.
Claim 11
Dependent Claim
25 words
A process according to claim 1 wherein before transplantation cellular bodies of the donor are inserted within the zona pellicuda of a foster embroyonic cell.
Claim 12
Dependent Claim
25 words
A process according to claim 1 wherein before transplant to a recipient the developing cellular bodies are cultured at a temperature between 30*C. and 38*C.
Claim 13
Dependent Claim
26 words
A process according to claim 1 wherein the cellular bodies are cultured in a medium contain saline solution, glucose, amino acids and vitamins and sodium pyruvate.
Claim 14
Dependent Claim
19 words
A process according to claim 1 wherein the life support medium is in aCcordance with the preferred examples herein.
Claim 15
Dependent Claim
16 words
A process according to claim 1 wherein the cellular bodies are frozen and thawed before transplantation.
Claim 16
Dependent Claim
12 words
The differentiated cellular body derived by the process according to claim 1.
Claim 17
Independent Claim
108 words
A method of increasing the number of progeny of female herbivorous and omivorous hoofed mammal donors comprising, inducing superovulation in a desired donor by administration of gonadotrophin to obtain a plurality of ova at ovulation, recovering the ova from the donor, selecting an ovum from said recovered ova and dividing said ovum into a plurality of separate embryonic cells such that each is divided into a plurality of separate embryonic cellular bodies derived from said one selected ovum of the ova which have been recovered, and transplanting one or more of the said cellular bodies derived from said one selected ovum to a recipient foster female for maturation.
Claim 18
Independent Claim
108 words
A method of increasing the number of progeny of female herbivorous and omivorous hoofed mammal donors comprising, inducing superovulation in a desired donor by administration of gonadotrophin to obtain a plurality of ova at ovulation, recovering the ova from the donor, selecting an ovum from said recovered ova and dividing said ovum into a plurality of separate embryonic cells such that each is divided into a plurality of separate embryonic cellular bodies derived from said one selected ovum of the ova which have been recovered, and transplanting one or more of the said cellular bodies derived from said one selected ovum to a recipient foster female for maturation.
Claim 19
Dependent Claim
30 words
A method according to claim 16 wherein the ova of the donor are fertilized with a spermatazoa fraction of which the greater number will produce zygotes of a desired sex.
Claim 20
Dependent Claim
25 words
A method according to claim 16 wherein the cellular bodies are sexed before transplantation to a recipient, the recipient receiving only transplants of like sex.
Claim 21
Dependent Claim
16 words
A process according to claim 1 wherein the ova are recovered after slaughter of the donor.
Claim 22
Dependent Claim
14 words
A process according to claim 16 wherein the removed ova are fertilized in vitro.
Claim 23
Dependent Claim
13 words
A process according to claim 1 wherein the transplantation is accomplished without surgery.
Claim 24
Dependent Claim
11 words
The differentiated cellular body derived by the method of claim 16.
Claim 25
Dependent Claim
145 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, storing ova recovered from a donor in tissue culture medium as individual ovum cells or cellular bodies for a period of time, preparing one or more recipients of a prospective transplantation and determining the time of ovulation in a selected recipient, selecting a stored ovum or cellular body from said storage tissue culture medium, and, transplanting said selected ovum or cellular body derived from said donor to said selected recipient at a time corresponding to a synchronization of the individual ovum or cellular body transplanted and the time of normal fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own had the recipient been fertilized.
Claim 26
Dependent Claim
145 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, storing ova recovered from a donor in tissue culture medium as individual ovum cells or cellular bodies for a period of time, preparing one or more recipients of a prospective transplantation and determining the time of ovulation in a selected recipient, selecting a stored ovum or cellular body from said storage tissue culture medium, and, transplanting said selected ovum or cellular body derived from said donor to said selected recipient at a time corresponding to a synchronization of the individual ovum or cellular body transplanted and the time of normal fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own had the recipient been fertilized.
Claim 27
Dependent Claim
17 words
A process according to claim 23 wherein the ova are frozen with tissue culture medium before transplantation.
Claim 28
Dependent Claim
58 words
A process according to claim 23 wherein the ova recovered from the donor have cells separated from individual recovered ovum as embryonic cellular bodies and the development of each thus separated cellular body has its development to the blastocyst stage thus delayed, and subsequently one or more of the separated cellular bodies are transplanted to a selected recipient.
Claim 29
Dependent Claim
29 words
A process according to claim 23 wherein transplantation to the recipient occurs after the recovered ova have been stored for a period of time exceeding a plurality of days.
Claim 30
Dependent Claim
39 words
A process according to claim 23 wherein transplantation to the recipient is delayed for a period of time exceeding that time during which the donor would have normally transported its ova through its oviduct and into its uterine horn.
Claim 31
Dependent Claim
16 words
A process according to claim 23 wherein transplantation is to the uterine horn of the recipient.
Claim 32
Dependent Claim
15 words
A process according to claim 23 wherein transplantation is to the oviduct of the recipient.
Claim 33
Dependent Claim
28 words
A process according to claim 29 wherein transplantation is to the oviduct of the recipient via the uterine horn and via a cannula inserted through the isthmic junction.
Claim 34
Dependent Claim
62 words
A process according to claim 23 wherein the ovum is permitted division into a plurality of cellular bodies no further develoPed than the blastocyst stage, and one or more differentiated cells from a cellular body to be transplanted or from a cellular body derived from one which is to be transplanted are examined with a microscope to determine sex of the transplant.
Claim 35
Dependent Claim
31 words
A process according to claim 23 wherein the ova are recovered from a donor which has been administered luteinizing hormone 4 to 6 days after a gonodotrophin administration to cause superovulation.
Claim 36
Dependent Claim
28 words
A process according to claim 23 wherein the ova are recovered from a donor which has been fed a high energy ration during the period of follicular development.
Claim 37
Dependent Claim
35 words
A process according to claim 23 wherein before transplantation one or more of cellular bodies seperated from a single recovered ovum of the donor are inserted within the zona pellicuda of a foster embryonic cell.
Claim 38
Dependent Claim
26 words
A process according to claim 34 wherein the separated cellular bodies are separated from other cells of the same ovum by exposure to a lysin solution.
Claim 39
Dependent Claim
22 words
A process according to claim 23 wherein the period of time for storage of the recovered ovum is greated than 2 days.
Claim 40
Dependent Claim
20 words
A process according to claim 23 wherein the storage temperature of the tissue culture medium is between 30*C. and 38*C.
Claim 41
Dependent Claim
31 words
A process according to claim 23 wherein the individual ovum or cellular bodies are stored and cultured in a medium containing saline solution, glucose, amino acids and vitamins and sodium pyruvate.
Claim 42
Dependent Claim
21 words
A process according to claim 23 wherein the lift support tissue culture medium is in accordance with the preferred examples herein.
Claim 43
Dependent Claim
37 words
A process according to claim 23 wherein said recovered ova are obtained by inducing superovulation in a desired donor by administration of gonadotrophin to said donor, and subsequently after ovulation the ova are recovered from said donor.
Claim 44
Dependent Claim
30 words
A process according to claim 23 wherein the ova of the donor are fertilized with a spermatazoa fraction of which the greater number will produce zygotes of a desired sex.
Claim 45
Dependent Claim
38 words
A process according to claim 23 wherein the transplanted cellular bodies or ovum are sexed before transplantation to a recipient, the recipient receiving only individual ovum or cellular bodies of a selected sex and of the same sex.
Claim 46
Dependent Claim
17 words
A process according to claim 23 wherein the recovered ova are recovered after slaughter of the donor.
Claim 47
Dependent Claim
14 words
A process according to claim 23 wherein the recovered ova are fertilized in vitro.
Claim 48
Dependent Claim
13 words
A process according to claim 23 wherein the transplantation is accomplished without surgery.
Claim 49
Dependent Claim
29 words
A process according to claim 23 wherein the individual ovum or cellular bodies are a clonal genotype, each embryonic cell having all its chromozones derived from the same individual.
Claim 50
Dependent Claim
47 words
A process according to claim 23 wherein the transplanted ovum or cellular body has all chromozones derived from the donor by inducing development of an oocyte recovered from the donor such that the oocyte is formed from a diploid nucleus derived from two genomes of the donor.
Claim 51
Dependent Claim
45 words
A process according to claim 23 wherein the donor of the recovered ova has been administered a high energy ration during the period of follicular development and during the period of follicular development pituitary gonadotrophin has been administered, and subsequently said ova have been recovered.
Claim 52
Dependent Claim
12 words
The differentiated cellular body derived by the process according to claim 23.
Claim 53
Dependent Claim
98 words
A process according to claim 23 wherein one or more recipients are prepared for a prospective transplantation by suppression of oestrus in said recipients by administration of progesterone to said recipients for a period of time, withdrawing the progesterone, subsequently administering gonadotrophin to enhance follicular development in said recipients, administering luteinizing hormone to the prospective recipients 4 to 6 days after the administration of gonadotrophin, and subsequently, performing said transplantation to said recipient 12 to 48 hours after administration of said luteinizing hormone or the detection of oestrus corresponding to the time of normal fertilization of the recipient.
Claim 54
Dependent Claim
36 words
A process according to claim 23 wherein the preparation of recipients includes the suppression of oestrus in said recipients and then ceasing said suppression of oestrus while currently therewith or thereafter administering estradiol to said recipient.
Claim 55
Dependent Claim
21 words
A process according to claim 23 wherein estradiol benzoate is administered to said recipients prior to performing the step of transplantation.
Claim 56
Dependent Claim
37 words
A process according to claim 23 wherein C-methyl-17 acetoxy progesterone is administered to a plurality of prospective recipients to suppress oestrus and permit synchronization of recipients and their period of ovulation with the time of desired transplantation.
Claim 57
Dependent Claim
44 words
A process according to claim 23 wherein during the period of preparation of the prospective recipients oestrus is suppressed for a period of time by the administration of progesterone as an oral dosage of .4 mg. to 1.0 mg. daily during said suppression period.
Claim 58
Dependent Claim
47 words
A process according to claim 23 wherein the preparation of prospective recipients includes the suppression of oestrus for a period of time and thereafter oestrus is permitted to occur and after said last oestrus period on the 14th to 20th day the prospective recipient is administered gonadotrophin.
Claim 59
Dependent Claim
55 words
A process according to claim 41 wherein the spermatazoa fraction is produced by a process which includes, washing sperm recovered from a male donor and transferring the spermatazoa to a dilute fluid support solution; percolating the spermatazoa through an ion exchange material; and concentrating the product and transferring the concentrated product to a buffered solution.
Claim 60
Dependent Claim
21 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by percolating the spermatazoa through a cation exchange resin.
Claim 61
Dependent Claim
33 words
A process according to claim 41 wherein the spermatazoa is centrifuged through an ion exchange resin and fractioned by separating and utilizing the lower fraction which has been separated in the centrifugation step.
Claim 62
Dependent Claim
57 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by percolating the spermatazoa through an ion exchange material of the cation type and the resin is of the group consisting of: carboxylic divinyl benzene copolymers; products of copolymerication of methacylic acid with divinyl benzene; and, products of maleic anhydride with styrene and divinyl benzene.
Claim 63
Dependent Claim
23 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by percolating spermatazoa through an ion exchange resin having the structure:
Claim 64
Dependent Claim
24 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by percolating spermatazoa through an ion exchange resin that has the structure:
Claim 65
Dependent Claim
42 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by a process including subjecting the spermatazoa to a vacuum of 15 to 30 cm. pressure differential from the pressure at which the spermatazoa was recovered from a male donor.
Claim 66
Dependent Claim
35 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by a process including subjection the spermatazoa to a pressure greater than that at which the spermatazoa was recovered from a male donor.
Claim 67
Dependent Claim
54 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by a process including, washing and diluting spermatazoa recovered from a male donor, placing the dilute solution containing spermatazoa in a counterstreaming centrifuge and the centrifuging for a period exceeding 20 minutes, discontinuing centrifugation and removing the desired fraction from the dilutent.
Claim 68
Dependent Claim
30 words
A process according to claim 64 wherein during centrifugation a pressure differential from that pressure at which the spermatazoa were recovered from a malE donor is applied to the spermatazoa.
Claim 69
Dependent Claim
18 words
A process according to claim 65 wherein the pressure differential is a vacuum of 15 to 30 cm.
Claim 70
Dependent Claim
23 words
A process according to claim 64 wherein there is an ion exchange resin within the centrifuge through which the spermatazoa percolate during centrifugation.
Claim 71
Dependent Claim
28 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by a process including subjecting the spermatazoa to centrifugation at a speed less than 1000 r.p.m.
Claim 72
Dependent Claim
34 words
A process according to claim 41 wherein the spermatazoa fraction is obtained by a process including subjection of the spermatazoa to centrifugation at a speed greater than 1000 r.p.m and less than 1200 r.p.m.
Claim 73
Dependent Claim
118 words
A process according to claim 23 wherein individual ovum or cellular bodies intended for transplantation are stored for a period of time by a process including, transferring an individual ovum or cellular body of the recovered ova having developed no further than the morula stage of differentiation in a small amount of fluid to a life support medium and container therefor, cooling the container in a bath of fluid at a rate of less than 2*C. per minute, at approximately 0*C. adding a like amount of buffer solution to said container, then continuing the cooling of the container at a like rate of cooling until a stable frozen storage temperature for the ovum or cellular body is reached.
Claim 74
Dependent Claim
31 words
A process according to claim 70 wherein at approximately minus 4*C. the container is subjected to further cooling after seeding the fluid bath with a frozen crystal to initiate further cooling.
Claim 75
Dependent Claim
21 words
A process according to claim 70 wherein the zone pellicuda of the cellular body is removed before transfer to said container.
Claim 76
Dependent Claim
70 words
A process according to claim 24 wherein the frozen ova includes an oosphere which is subsequently thawed to approximately 0*C. and successive drops of sperm containing fluid is mixed with the culture medium until the amount of fluid medium is approximately .8 ml to 1 ml., and the medium is allowed to warm by exposure of its contained to air of normal ambient temperature for a period exceeding 3 hours.
Claim 77
Dependent Claim
43 words
A process according to claim 23 wherein a plurality of developing embryonic cellular bodies or ovum are transplanted to a single recipient, the number being transplanted being determined by the uterine capacity of the recipient so as not to exceed the uterine capacity.
Claim 78
Dependent Claim
116 words
A process for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, selecting a single ovum which has been recovered from a donor for transplantation, separating one or more cells from other cells of said single ovum to obtain a plurality of like but separate embryonic cellular bodies which are separated and freed from other cells of said single ovum, selecting individual seperated cellular bodies which have thus been separated and are capable of individual differentiation for transplantation, and transplanting one or more of the selected individual separated cellular bodies which are capable of further differentiation and derived from the said single ovum to a recipient female for further maturation therein.
Claim 79
Dependent Claim
116 words
A process for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, selecting a single ovum which has been recovered from a donor for transplantation, separating one or more cells from other cells of said single ovum to obtain a plurality of like but separate embryonic cellular bodies which are separated and freed from other cells of said single ovum, selecting individual seperated cellular bodies which have thus been separated and are capable of individual differentiation for transplantation, and transplanting one or more of the selected individual separated cellular bodies which are capable of further differentiation and derived from the said single ovum to a recipient female for further maturation therein.
Claim 80
Dependent Claim
29 words
A process according to claim 75 wherein the selected ovum has been fertilized with a spermatazoa fraction of which the greated number will produce zygotes of a desired sex.
Claim 81
Dependent Claim
17 words
A process according to claim 75 wherein the cellular bodies are sexed before transplantation to a recipient.
Claim 82
Dependent Claim
29 words
A process according to claim 75 wherein the individual embryonic cells are separated and freed from other cells of said single ovum with the aid of a lysin solution.
Claim 83
Dependent Claim
40 words
A process according to claim 75 wherein said single ovum is asexually prepared from an oosphere recovered from the donor and has its chromozones all derived from its donor parent before said embryonic cells are separated from one another. Pg,65
Claim 84
Dependent Claim
41 words
A process according to claim 75 wherein said transplanted separated cellular bodies are inserted within the zona pellicuda of a waiting ovum having its nuclear material destroyed before said one or more selected individual cellular bodies is transplanted to a recipient.
Claim 85
Dependent Claim
32 words
A process according to claim 75 wherein said transplanted separated cellular bodies are protected before transplantation by injecting the necular material of the separated cell into a foster cell which is ennucleated.
Claim 86
Dependent Claim
12 words
A differentiated cellular body derived according to the process of claim 75.
Claim 87
Dependent Claim
39 words
A process according to claim 75 wherein the donor has been superovulated and a plurality of ova recovered from the donor and one or more of the recovered ova are processed in accordance with the steps of claim 75.
Claim 88
Dependent Claim
29 words
A process according to claim 75 wherein the recipient has been administered gonadothrophin prior to the oestrus during which the transplantation of embryonic cells from the donor is performed.
Claim 89
Dependent Claim
197 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, recovering an ovum from said donor, separating one or more cells from said ovum after said ovum has divided but differentiated no further than the morula stage of development with the aid of a lysin solution into which the said ovum is placed and individual cells separated from other cells of the said ovum, thereby freeing individual embryonic cellular bodies which are capable of separate differentiation, injecting individual cells which have been thus separated into a corresponding waiting waiting cellular body such that said waiting cellular body protects the injected individual cell from destruction and permits the individual cell to develop and differentiate as a seperate embryonic cell which is retarded in development as compared with the stage of development said original ovum would have reached had said individual cells not been seperated therefrom, and subsequently transplanting one or more thus separated individual cells derived from the one ovum obtained from the donor to a recipient at a time such that the recipient is synchronized to receive the transplanted cells in accordance with their stage of differentiation.
Claim 90
Dependent Claim
197 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, recovering an ovum from said donor, separating one or more cells from said ovum after said ovum has divided but differentiated no further than the morula stage of development with the aid of a lysin solution into which the said ovum is placed and individual cells separated from other cells of the said ovum, thereby freeing individual embryonic cellular bodies which are capable of separate differentiation, injecting individual cells which have been thus separated into a corresponding waiting waiting cellular body such that said waiting cellular body protects the injected individual cell from destruction and permits the individual cell to develop and differentiate as a seperate embryonic cell which is retarded in development as compared with the stage of development said original ovum would have reached had said individual cells not been seperated therefrom, and subsequently transplanting one or more thus separated individual cells derived from the one ovum obtained from the donor to a recipient at a time such that the recipient is synchronized to receive the transplanted cells in accordance with their stage of differentiation.
Claim 91
Dependent Claim
13 words
The differentiated cellular body in accordance with the process according to claim 85.
Claim 92
Dependent Claim
258 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, selecting a desired donor, administering gonadotrophin to said donor to induce superovulation of said donor, detecting oestrus in said donor, and inseminating said donor, recovering a plurality of fertilized ova from said donor and storing said ova recovered from said donor in tissue culture medium at a temperature between 30*C. and 38*C. selecting a plurality of prosepective recipients which are expected to ovulate at approximately the same time and injecting said prospective recipients with luteinizing hormone at approximately the same time, selecting a stored ovum which has undergone several divisions and separating several of the cells of said selected ovum and freeing them from one another so as to provide a plurality of embryonic cells capable of further division and segregating said separate cells into groups originating from said selected ovum withing 12 to 48 hours after the administration of luteinizing hormone transplanting one or more of said segregated and separated cells derived from said selected stored ovum to a selected recipient at a time corresponding to a synchronization of the said 12 to 48 hour period after hormone administration and a 12 to 48 hour period of development of each separated individual cell to be transplanted after said seperation and such that the transplant is synchronized at the blastocyst stage in the recipient at the time the Recipient would normally implant a blastocyst of its own, and repeating the transplantion for other cells in the same manner.
Claim 93
Dependent Claim
258 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, selecting a desired donor, administering gonadotrophin to said donor to induce superovulation of said donor, detecting oestrus in said donor, and inseminating said donor, recovering a plurality of fertilized ova from said donor and storing said ova recovered from said donor in tissue culture medium at a temperature between 30*C. and 38*C. selecting a plurality of prosepective recipients which are expected to ovulate at approximately the same time and injecting said prospective recipients with luteinizing hormone at approximately the same time, selecting a stored ovum which has undergone several divisions and separating several of the cells of said selected ovum and freeing them from one another so as to provide a plurality of embryonic cells capable of further division and segregating said separate cells into groups originating from said selected ovum withing 12 to 48 hours after the administration of luteinizing hormone transplanting one or more of said segregated and separated cells derived from said selected stored ovum to a selected recipient at a time corresponding to a synchronization of the said 12 to 48 hour period after hormone administration and a 12 to 48 hour period of development of each separated individual cell to be transplanted after said seperation and such that the transplant is synchronized at the blastocyst stage in the recipient at the time the Recipient would normally implant a blastocyst of its own, and repeating the transplantion for other cells in the same manner.
Claim 94
Dependent Claim
245 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium; after recovery of the ova from the donor retarding development through differentiation of the ova by a retardation process, said retardation process including a step of freezing the one or more recovered embryonic ova with a small amount of life support medium made available in a container, cooling the container in a fluid bath at a rate of less than 2*C. per minute and adding to said container at approximately 0*C. a like small mount of buffer solution and then continuing the cooling of the container to a temperature below 0*C. which is a stable storage temperature; detecting oestrus in a prospective recipient, selecting a stored ovum from said stored frozen ova and thawing said stored ovum which has been selected, and transplanting said selected ovum to said prospective recipient at a time corresponding to a synchronization of said selected ovum and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 95
Dependent Claim
245 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium; after recovery of the ova from the donor retarding development through differentiation of the ova by a retardation process, said retardation process including a step of freezing the one or more recovered embryonic ova with a small amount of life support medium made available in a container, cooling the container in a fluid bath at a rate of less than 2*C. per minute and adding to said container at approximately 0*C. a like small mount of buffer solution and then continuing the cooling of the container to a temperature below 0*C. which is a stable storage temperature; detecting oestrus in a prospective recipient, selecting a stored ovum from said stored frozen ova and thawing said stored ovum which has been selected, and transplanting said selected ovum to said prospective recipient at a time corresponding to a synchronization of said selected ovum and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 96
Dependent Claim
253 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium; after recovery of the ova from the donor retarding development of the recovered ova by a retardation process, said retardation process including a step of selecting an ovum from said recovered ova and separating one or more cells from said ovum after said ovum has divided but differentiated no further than the morula stage of development with the aid of a lysin solution into which said ovum is placed and individual cells separated from other cells of the said selected ovum, thereby freeing individual embryonic cellular bodies which are capable of seperate differentiation, and storing said cellular bodies in tissue culture medium for a period of time; detecting oestrus in a prospective recipient, selecting a stored cellular body from said culture medium, and transplanting said selected cellular body to said prospective recipient at a time corresponding to a synchronization of said selected cellular body at its stage of development and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 97
Dependent Claim
253 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium; after recovery of the ova from the donor retarding development of the recovered ova by a retardation process, said retardation process including a step of selecting an ovum from said recovered ova and separating one or more cells from said ovum after said ovum has divided but differentiated no further than the morula stage of development with the aid of a lysin solution into which said ovum is placed and individual cells separated from other cells of the said selected ovum, thereby freeing individual embryonic cellular bodies which are capable of seperate differentiation, and storing said cellular bodies in tissue culture medium for a period of time; detecting oestrus in a prospective recipient, selecting a stored cellular body from said culture medium, and transplanting said selected cellular body to said prospective recipient at a time corresponding to a synchronization of said selected cellular body at its stage of development and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 98
Dependent Claim
208 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium, determining the sex of each of the recovered ova by a microscopic examination of one or more cells of each ovum, thereafter retarding development of the recovered ova by a retardaTion process and storing said recovered and retarded ova in a life support medium for a period of time, detecting oestrus in a prospective recipient, selecting a stored ovum or a plurrlity of stored ova from said stored ova, all selected ova being of like sex, and transplanting said selected ovum or plurality of stored ova of like sex to said prospective recipient at a time corresponding to a synchronization of said selected ovum and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 99
Dependent Claim
208 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium, determining the sex of each of the recovered ova by a microscopic examination of one or more cells of each ovum, thereafter retarding development of the recovered ova by a retardaTion process and storing said recovered and retarded ova in a life support medium for a period of time, detecting oestrus in a prospective recipient, selecting a stored ovum or a plurrlity of stored ova from said stored ova, all selected ova being of like sex, and transplanting said selected ovum or plurality of stored ova of like sex to said prospective recipient at a time corresponding to a synchronization of said selected ovum and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 100
Dependent Claim
207 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor with spermatazoa from a fraction of spermatazoa of which the greater number will produce zygotes of a desired sex, recovering fertilized ova from said donor by flushing the said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium, examining one or more cells of the recovered ova to determine the sex of the cells of the ovum from which the cells being examined are derived and segregating the embryonic ova in accordance with their sex, subjecting said recovered ova to a retardadation process which retards normal development of the ova and storing said recovered ova for a period of time, detecting oestrus in a prospective recipient, selecting one or more stored ova of like sex from storage, and transplanting said selected ovum to said prospective recipient at a time such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 101
Dependent Claim
207 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor with spermatazoa from a fraction of spermatazoa of which the greater number will produce zygotes of a desired sex, recovering fertilized ova from said donor by flushing the said ova from the oviduct or uterus of the donor and removing said flushed ova from the donor and placing the recovered ova in a life support medium, examining one or more cells of the recovered ova to determine the sex of the cells of the ovum from which the cells being examined are derived and segregating the embryonic ova in accordance with their sex, subjecting said recovered ova to a retardadation process which retards normal development of the ova and storing said recovered ova for a period of time, detecting oestrus in a prospective recipient, selecting one or more stored ova of like sex from storage, and transplanting said selected ovum to said prospective recipient at a time such that the transplant is synchronized at the blastocyst stage in the recipient at the time the recipient would normally implant a blastocyst of its own.
Claim 102
Dependent Claim
283 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing the said ova from the oviduct or uterus of the donor and removing said flushed ova and placing the recovered ova in a life support medium, examining one or more cells of the recovered ova to determine the sex of the cells of the ovum from which the cells being examined are derived and segregating the embryonic ova in accordance with their sex, subjecting said recovered ova to a retardation process which retards normal development of the ova and permits prolonged storage of the recovered ova, said retardation process including a step of freezing one or more recovered embryonic ova with a small amount of life support medium made available in a container, cooling the container in a fluid bath at a rate of less than 2*C. per minute and adding to said container at approximately 0*C. a like small amount of buffer solution and then continuing the cooling of the container to a temperature below 0*C. which is a stable storage temperature; detecting oestrus in a prospective recipient, selecting from storage one or more recovered ova of like sex and thawing said selected ova, and transplanting said selected ovum or ova to said recipient at a time corresponding to a synchronization of said selected ova and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time that the recipient would normally implant a blastocyst of its own.
Claim 103
Dependent Claim
283 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor, detecting oestrus in said donor, inseminating said donor, recovering fertilized ova from said donor by flushing the said ova from the oviduct or uterus of the donor and removing said flushed ova and placing the recovered ova in a life support medium, examining one or more cells of the recovered ova to determine the sex of the cells of the ovum from which the cells being examined are derived and segregating the embryonic ova in accordance with their sex, subjecting said recovered ova to a retardation process which retards normal development of the ova and permits prolonged storage of the recovered ova, said retardation process including a step of freezing one or more recovered embryonic ova with a small amount of life support medium made available in a container, cooling the container in a fluid bath at a rate of less than 2*C. per minute and adding to said container at approximately 0*C. a like small amount of buffer solution and then continuing the cooling of the container to a temperature below 0*C. which is a stable storage temperature; detecting oestrus in a prospective recipient, selecting from storage one or more recovered ova of like sex and thawing said selected ova, and transplanting said selected ovum or ova to said recipient at a time corresponding to a synchronization of said selected ova and the time of fertilization of the recipient such that the transplant is synchronized at the blastocyst stage in the recipient at the time that the recipient would normally implant a blastocyst of its own.
Claim 104
Dependent Claim
327 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulatin in a desired donor, detecting oestrus in said donor, inseminating said donor, recOvering fertilized ova from said donor by flushing the said ova from the oviduct or uterus of the donor and removing said flushed ova and placing the recovered ova in a life support medium, examining one or more cells of the recovered ova to determine the sex of the cells of the ovum from which the cells being examined are derived and segregating the embryonic ova in accordance with their sex, subjecting said recovered ova to a retardation process which retards normal development of the ova and permits prolonged storage of the recovered ova, said retardation process including separating one or more cellular bodies from a single ovum after the single ovum has divided but differentiated no further than the morula stage of development to seperate and free individual embryonic cellular bodes from each other which are of the same sex and derived from the same individual and are capable of sperate differentiation but retarded in their stage of division as compared to the stage which would have been reached by the single ovum from which they are derived, and freezing said recovered ova or cellular bodies at a cooling rate of less than 2*C. per minute with a small amount of buffer solution added at approximately 0*C. and then the cooling is continued to a stable storage temperature below freezing at approximately the same rate of cooling, and subsequently selecting one or more ova or cellular bodies of the same sex and thawing the same and then transplanting said selected ova or cellular bodies to a recipient at a time such that the transplant is synchronized at the blastocyst stage in the recipient at the time that the recipient would normally implant a blastocyst of its own during normal development after fertilization.
Claim 105
Dependent Claim
327 words
A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulatin in a desired donor, detecting oestrus in said donor, inseminating said donor, recOvering fertilized ova from said donor by flushing the said ova from the oviduct or uterus of the donor and removing said flushed ova and placing the recovered ova in a life support medium, examining one or more cells of the recovered ova to determine the sex of the cells of the ovum from which the cells being examined are derived and segregating the embryonic ova in accordance with their sex, subjecting said recovered ova to a retardation process which retards normal development of the ova and permits prolonged storage of the recovered ova, said retardation process including separating one or more cellular bodies from a single ovum after the single ovum has divided but differentiated no further than the morula stage of development to seperate and free individual embryonic cellular bodes from each other which are of the same sex and derived from the same individual and are capable of sperate differentiation but retarded in their stage of division as compared to the stage which would have been reached by the single ovum from which they are derived, and freezing said recovered ova or cellular bodies at a cooling rate of less than 2*C. per minute with a small amount of buffer solution added at approximately 0*C. and then the cooling is continued to a stable storage temperature below freezing at approximately the same rate of cooling, and subsequently selecting one or more ova or cellular bodies of the same sex and thawing the same and then transplanting said selected ova or cellular bodies to a recipient at a time such that the transplant is synchronized at the blastocyst stage in the recipient at the time that the recipient would normally implant a blastocyst of its own during normal development after fertilization.
Claim 106
Dependent Claim
12 words
A differentiated cellular body derived by the process according to claim 87.
Claim 107
Dependent Claim
12 words
A differentiated cellular body derived by the process according to claim 88.
Claim 108
Dependent Claim
12 words
A differentiated cellular body derived by the process according to claim 89.
Claim 109
Dependent Claim
12 words
A differentiated cellular body derived by the process according to claim 90.
Claim 110
Dependent Claim
12 words
A differentiated cellular body derived by the process according to claim 91.
Claim 111
Dependent Claim
12 words
A differentiated cellular body derived by the process according to claim 92.
Claim 112
Dependent Claim
12 words
A differentiated cellular body derived by the process according to claim 93.
References Cited (Backward Citations)
US3574829A
Cited
US3687806A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-16 | 1978-06-17 | 1978-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-16 | 1982-06-17 | 1982-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-16 | 1986-06-17 | 1986-12-17 | $7700.00 | Window Closed / Lapsed |