US3789116A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorescent labeled antibody reagent
Assignee: Abbott Laboratories
•
Filing: 1970-12-09
•
Granted: 1974-01-29
•
Expires: 1990-12-09
Abstract
Abstract A lyophilized stable labeled antibody reagent that comprises a non-reducing polysaccharide, a normal serum and a labeled antibody, particularly an antibody labeled with a fluorescent chemical is provided that is exceptionally specific to a given antigen. Specificity is such that Group A, Beta -hemolytic Streptococci, can readily be identified in the presence of Group C or Group G Streptococci or Staphlococcus aureus. The reagent is stable in the lyophilized state at temperatures of up to 37*C. for a period of months and in the reconstituted state at 2* to 8* C. for a period of weeks.
Inventors
- 👤 J Kay
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
13 words
The reagent of claim 1 in which said labeling agent is fluorescein isothiocyanate.
Claim 2
Dependent Claim
11 words
The reagent of claim 1 in which said polysaccharide is sucrose.
Claim 3
Dependent Claim
11 words
The reagent of claim 1 in which said preservative is thimerosol.
Claim 4
Dependent Claim
14 words
The reagent of claim 1 in which said isotonic solution is phosphate buffered saline.
Claim 5
Dependent Claim
70 words
A reagent solution suitable for lyophilization comprising an admixture of at least 0.05 percent monospecific fluorescent labeled antibody for the detection of Group A streptococci, 10 75 percent polysaccharide selected from the group consisting of sucrose and fructose, 20 to 55 percent normal serum derived from the same host species as that of the monospecific antibody; and a quantity sufficient of a preservative, said admixture combined with an isotonic carrier.
Claim 6
Dependent Claim
56 words
A lyophilized reagent comprising at least 0.05 percent of a monospecific fluorescent labeled antibody, specific to Group A streptococci, 10 to 75 percent of a polysaccharide selected from the group consisting of sucrose and fructose, and 20 to 55 percent of a normal serum derived from the same host species as that of the monospecific antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-01-28 | 1977-07-30 | 1978-01-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-01-28 | 1981-07-30 | 1982-01-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-01-28 | 1985-07-30 | 1986-01-29 | $7700.00 | Window Closed / Lapsed |