US3683550A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plant culture method and product
Assignee: Big Heart Pet Brands LLC
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Filing: 1970-04-01
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Granted: 1972-08-15
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Expires: 1990-04-01
Abstract
Abstract A method for reproducing large numbers of pineapple plants using a three stage plant tissue culture technique. Also the product of latent embryoid tissue (i.e., bud clusters) used in the same technique. In an induction stage, latent embryoid tissue is derived and induced to grow from an excised plant meristem. Such tissue is then grown or proliferated in an aqueous nutrient medium under conditions whereby differentiation of tissue into plantlets is inhibited. In a preferred method, the latent embryoid tissue is proliferated to a desirable amount and then transferred to a storage bank for unlimited storage. When desired, a portion of tissue may be removed from the bank and further proliferated. Also, the storage bank product. Then, in a differentiation stage, the latent embryoid tissue is caused to differentiate into plantlets.
Inventors
- 👤 Donald A Corlett Jr
- 👤 Panos D Caldis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:04
CPC Classifications
A; A01; A01H; A01H4/00; A01H4/005
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
62 words
A method for producing a pineapple plant culture comprising removing meristem tissue from a donor plant, incising the meristem tissue to promote growth of bud clusters therefrom, said growth occurring while the meristem is in aqueous nutrient medium, and subjecting the bud clusters to predetermined environmental conditions in an aqueous nutrient medium to proliferate the bud clusters without substantial differentiation into plantlets.
Claim 2
Independent Claim
62 words
A method for producing a pineapple plant culture comprising removing meristem tissue from a donor plant, incising the meristem tissue to promote growth of bud clusters therefrom, said growth occurring while the meristem is in aqueous nutrient medium, and subjecting the bud clusters to predetermined environmental conditions in an aqueous nutrient medium to proliferate the bud clusters without substantial differentiation into plantlets.
Claim 3
Dependent Claim
22 words
A method as in claim 1 in which the promoted bud clusters are separated from any remaining donor tissue prior to proliferation.
Claim 4
Dependent Claim
25 words
A method as in claim 1 in which the bud clusters are subjected to modified environmental conditions to cause the same to differentiate into plantlets.
Claim 5
Dependent Claim
19 words
A method as in claIm 1 wherein the meristem tissue removed from the donor plant is a bud meristem.
Claim 6
Dependent Claim
31 words
A method as in claim 1 wherein the aqueous nutrient medium contains a carbohydrate, vitamins, trace metallic elements, and ions of potassium, magnesium, calcium, nitrate, phosphate, sulfate, and chelated ferrous ion.
Claim 7
Dependent Claim
70 words
A method as in claim 1 together with the steps of storing the proliferated bud clusters in a container bank with sufficient aqueous nutrient medium to substantially cover the bud clusters, maintaining the container bank at conditions whereby differentiation into plantlets is inhibited, agitating the container bank, removing the medium and upper portion of the bud clusters periodically and adding sufficient new medium to again cover the remaining bud clusters.
Claim 8
Dependent Claim
48 words
A method as in claim 1 wherein the aqueous nutrient medium contains vitamins, a carbohydrate, trace metallic elements and ions of potassium, magnesium, calcium, nitrate, phosphate, sulfate, and at least about 5 mg/l of chelated ferrous ion and polyvinylpyrrolidone in the quantity of about 50-100 mg/l of medium.
Claim 9
Independent Claim
89 words
A method for reproducing large numbers of pineapple plants from pineapple bud clusters comprising subjecting said bud clusters to predetermined environmental conditions while substantially submerged in an aqueous nutrient medium to proliferate the bud clusters into a mass without substantial differentiation into plantlets, and thereafter exposing a substantial upper surface portion of the proliferated bud cluster mass to the atmosphere and simultaneously contacting a lower surface of the bud cluster mass with aqueous nutrient medium to cause an upper segment of the proliferated bud clusters to differentiate into plantlets.
Claim 10
Independent Claim
89 words
A method for reproducing large numbers of pineapple plants from pineapple bud clusters comprising subjecting said bud clusters to predetermined environmental conditions while substantially submerged in an aqueous nutrient medium to proliferate the bud clusters into a mass without substantial differentiation into plantlets, and thereafter exposing a substantial upper surface portion of the proliferated bud cluster mass to the atmosphere and simultaneously contacting a lower surface of the bud cluster mass with aqueous nutrient medium to cause an upper segment of the proliferated bud clusters to differentiate into plantlets.
Claim 11
Dependent Claim
25 words
A method as in claim 8 wherein said medium employed for differentiation includes substantially the same type of nutrient as the medium employed for proliferation.
Claim 12
Dependent Claim
27 words
A method as in claim 9 in which the nutrient concentration in the proliferation medium is reduced by about 50 to 90 percent in the differentiation medium.
Claim 13
Dependent Claim
22 words
A method as in claim 9 wherein the nutrient concentration is reduced by about 70 to 80 percent in the differentiation medium.
Claim 14
Dependent Claim
28 words
A method as in claim 9 in which the nutrient medium includes carbohydrates and trace metallic elements, and ions of iron, potassium, magnesium, calcium, nitrate, phosphate and sulfate.
Claim 15
Dependent Claim
28 words
A method as in claim 8 in which the proliferation medium nutrients include about 1.5 to 3 percent by weight of carbohydrate along with adequate vitamins and minerals.
Claim 16
Dependent Claim
27 words
A method as in claim 8 in which the differentiation medium nutrients include 0.25 to 1 percent by weight of carbohydrate along with adequate vitamins and minerals.
Claim 17
Dependent Claim
21 words
A method as in claim 8 wherein differentiation into plantlets is performed on a soft inert gel containing aqueous nutrient medium.
Claim 18
Dependent Claim
35 words
A method as in claim 15 wherein the gel is formed of agar dispersed in the aqueous nutrient medium, said agar being present in a quantity of about 1 to 1.4 percent of the medium.
Claim 19
Dependent Claim
68 words
A culture comprising granular pineapple bud clusters free-living apart from the donor pineapple plant, at least a portion of the bud clusters being in contact with aqueous nutrient, said bud clusters being characterized by the capacity to proliferate to form more granules without substantial differentiation into plantlets under first predetermined environmental conditions and the capacity to differentiate into a plurality of pineapple plantlets by modifying the environmental conditions.
Claim 20
Dependent Claim
68 words
A culture comprising granular pineapple bud clusters free-living apart from the donor pineapple plant, at least a portion of the bud clusters being in contact with aqueous nutrient, said bud clusters being characterized by the capacity to proliferate to form more granules without substantial differentiation into plantlets under first predetermined environmental conditions and the capacity to differentiate into a plurality of pineapple plantlets by modifying the environmental conditions.
Claim 21
Dependent Claim
29 words
A culture as in claim 17 in which the first environmental conditions comprise submergence in liquid nutrient medium and the second environmental conditions comprise exposure to an oxygen-containing environment.
Claim 22
Dependent Claim
19 words
A culture as in claim 17 in which substantially all of the bud clusters are submerged in said medium.
Claim 23
Dependent Claim
36 words
A culture as in claim 19 in which an upper surface portion of the bud clusters is expoSed to the atmosphere and a lower surface portion of the same is in contact with aqueous nutrient medium.
Claim 24
Dependent Claim
51 words
A culture as in claim 20 in which a soft inert gel is dispersed in the aqueous nutrient medium and a lower surface portion of the bud clusters rests on the agar in contact with the medium while an upper surface portion of the bud clusters is exposed to the atmosphere.
References Cited (Backward Citations)
US3514900A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-08-15 | 1976-02-14 | 1976-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-08-15 | 1980-02-14 | 1980-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-08-15 | 1984-02-14 | 1984-08-15 | $7700.00 | Window Closed / Lapsed |