Apparatus for cell culture
Abstract
Abstract METHOD AND APPARATUS FOR HANDLING CELLS WHICH MAY BE MICROORGANISMS OR MAY BE DERIVED FROM THE TISSUES OF LIVING ORGANISMS. THE METHOD AND APPARATUS PERMIT GROWING SUCH CELLS IN AN ACTIVE GROWTH STATE TO POPULATION DENSITIES WHICH MAY BE FROM 2 TO 35 TIMES THE DENSITIES NOW POSSIBLE WITH PRESENT METHODS AND EQUIPMENT. THE CELLS ARE MAINTAINED SUSPENDED IN A LIQUID MEDIUM IN WHICH IS PLACED A ROTATING FILTER. LIQUIDS MAY BE INTRODUCED AND WITHDRAWN WITHOUT DISTRUBING THE CELLS. CELL DENSITIES MAY BE BUILT UP TO A HIGH LEVEL AND USED AS MODEL TISSUES TO DETERMINE THE EFFECT OF CHEMICAL AGENTS ON THEM UNDER CONDITIONS WHICH ARE SIMILAR TO THOSE IN LIVING ORGANISMS. THE METHOD AND APPARATUS ARE ALSO SUITABLE FOR CARRYING OUT LARGE-SCALE INDUSTRIAL PROCESS INCLUDING THE SYNTHESIS OF ORGANIC CHEMICALS AS WELL AS THE TREATMENT OF SEWAGE.
Inventors
- 👤 Dennis E Johnson
- 👤 Lawrence R Woodland
- 👤 Charles J Kensler
- 👤 Philip Himmelfarb
Bibliographic Details
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