US3625827A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Water-soluble polymer-enzyme products
Assignee: Monsanto Co
•
Filing: 1968-09-27
•
Granted: 1971-12-07
•
Expires: 1988-09-27
Abstract
Abstract Polymer-enzyme products wherein the enzyme is covalently bound to the polymer chain, the polymer-enzyme product being water soluble. The products have wide applicability as stable, longacting, enzymatic materials, having same general type of activity as parent enzyme, but with different pH optimum activity and range of applicability, and are stable, substantially colorless and odorless, long acting, and remarkably less subject to autogenous deterioration or destruction by other enzymes.
Inventors
- 👤 Bernard S Wildi
- 👤 Thomas L Westman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:09
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/18; C08; C08F; C08F8/00; C12N11/02; C12N11/08; C12N11/082
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
28 words
Product of claim 1, wherein the polymer is formed by polymerization of polymerizable acids or anhydrides, or by copolymerizing a polymerizable acid or anhydride with another copolymerizable monomer.
Claim 2
Dependent Claim
11 words
Product of claim 1, wherein the polymer is an EMA-type polymer.
Claim 3
Dependent Claim
9 words
Product of claim 3, wherein the polymer is EMA.
Claim 4
Dependent Claim
8 words
Product of claim 1, which is water-soluble EMA-lipase.
Claim 5
Dependent Claim
8 words
Product of claim 1, which is water-soluble EMA-cellulase.
Claim 6
Dependent Claim
8 words
Product of claim 1, which is water-soluble EMA-asparaginase.
Claim 7
Dependent Claim
9 words
Product of claim 1, which is water-soluble EMA-alkaline protease.
Claim 8
Dependent Claim
9 words
Product of claim 1, which is water-soluble EMA-neutral protease.
Claim 9
Dependent Claim
9 words
Product of claim 1, which is water soluble EMA-amylase.
Claim 10
Dependent Claim
20 words
Product of claim 1, wherein a plurality of different enzymes of microbiological origin are present in the water-soluble polymer-enzyme product.
Claim 11
Dependent Claim
13 words
Product of claim 1, which is a water-soluble EMA-alkaline protease, neutral protease product.
Claim 12
Dependent Claim
15 words
Product of claim 1, which is a water-soluble EMA-alkaline protease, neutral protease and amylase product.
Claim 13
Dependent Claim
15 words
Product of claim 1, which is a water-soluble EMA-alkaline protease, neutral protease and lipase product.
Claim 14
Dependent Claim
23 words
Product of claim 12, wherein the ratio of alkaline protease activity to neutral protease activity is about 0.25 to about 1.2 to 1.
Claim 15
Dependent Claim
8 words
Product of claim 1, which is water-soluble EMA-dextranase.
Claim 16
Dependent Claim
12 words
Product of claim 1, which is a water-soluble EMA-dextranase, neutral protease product.
Claim 17
Dependent Claim
219 words
Method of producing a water-soluble enzymatically active polymer-enzyme product wherein the enzyme is bound covalently through groups which are nonessential for enzymatic activity to (a) a water-soluble polymer comprising chains of carboxylic acid or carboxylic acid anhydride units, or (b) a water-soluble polymer comprising units of carboxylic acid or carboxylic acid anhydride groups separated by carbon chains of at least one and not more than four carbon atoms, said carbon chains being part of a unit which contains a maximum of 18 carbon atoms, which comprises the step of reacting the polymer and an enzyme under substantially noncross-linking conditions and according to conditions which do not operate to destroy enzymatic activity to produce a desired enzymatically active water-soluble polymer-enzyme product, wherein the polymer and enzyme are reacted together in dilute aqueous solution or suspension or with the enzyme in sufficient concentration to favor production of a noncrosslinked polymer-enzyme product, and wherein the polymer is selected from the group consisting of an ethylene/maleic anhydride copolymer, styrene/maleic anhydride copolymer, vinyl methyl ether/maleic anhydride copolymer, vinyl acetate/maleic anhydride copolymer, divinyl ether/maleic anhydride cyclocopolymer, polymaleic anhydride, polyacrylic anhydride, and cationic derivatives thereof and wherein the enzyme moiety comprises at least one enzyme of microbial origin selected from the group consisting of neutral protease, acid protease, alkaline protease, lipase, cellulase, dextranase, amylase and asparaginase.
Claim 18
Dependent Claim
35 words
Process of claim 18 wherein the polymer is reacted with a plurality of different enzymes of microbiological origin to produce a water-soluble polymer-enzyme product having a plurality of Different enzymes of microbiological origin bound therein.
References Cited (Backward Citations)
US3436309A
Cited
US3507750A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-12-06 | 1975-06-07 | 1975-12-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-12-06 | 1979-06-07 | 1979-12-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-12-06 | 1983-06-07 | 1983-12-07 | $7700.00 | Window Closed / Lapsed |