US3607650A
Expired / Lapsed
US
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Process for the enzymatic extraction and purification of a glycopeptide obtained from animal organs,useful as a drug
Assignee: Crinos Industria Farmacobiologica SpA
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Filing: 1970-05-15
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Granted: 1971-09-21
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Expires: 1990-05-15
Abstract
Abstract Glycopeptides are extracted from the gastric mucosa or duodenum of swine by hydrolysis of the animal organ under specified conditions, followed by separation of acidic hydrolysis byproducts and dilution of the extraction product with a nonsolvent to precipitate the glycopeptides.
Inventors
- 👤 Gianfranco Bertellini
- 👤 Adriano Butti
- 👤 Giuseppe Prino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:02
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C08B37/0066; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/843; Y10S530/844
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
28 words
Process of claim 1, wherein the product of (c) is dissolved in water, and the resulting aqueous solution is contacted with an acidic ion exchange resin before (d).
Claim 2
Dependent Claim
39 words
Process of claim 1, wherein the product of (d) is dissolved in water, the pH of the resulting solution is adjusted to a pH of 6 to 7, and the product is precipitated by addition of a nonsolvent therefor.
Claim 3
Dependent Claim
22 words
Process of claim 2, wherein contact with the acidic ion exchange resin is followed by contact with a basic ion exchange resin.
Claim 4
Dependent Claim
18 words
Process of claim 1, wherein the product is dialyzed at a pH from about 7 to about 8.
Claim 5
Dependent Claim
21 words
Process of claim 1, wherein the acidic byproducts of hydrolysis are precipitated by contacting the product of (a) with trichloroacetic acid.
Claim 6
Dependent Claim
17 words
Process of claim 1, wherein the pH of step (a) is from about 8.5 to about 9.5.
Claim 7
Dependent Claim
17 words
Process of claim 1, wherein the pH of step (a) is from about 1.5 to about 4.5.
Claim 8
Dependent Claim
13 words
Process of claim 13, wherein the nonsolvent is acetone or a lower alkanol.
Claim 9
Dependent Claim
77 words
A glycopeptide extracted from the gaStric mucosa or duodenum of swine having, by weight, a hexosamine content of from about 40 percent to 50 percent, a hexose content of from about 27 percent to 31 percent, an amino acid content of from about 13 percent to 18 percent, neuraminic acid derivatives of from about 4 percent to 6 percent, being substantially free from sulfur, phosphorus and uronic acids, and being prepared by the process of claim 1.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-09-20 | 1975-03-22 | 1975-09-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-09-20 | 1979-03-22 | 1979-09-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-09-20 | 1983-03-22 | 1983-09-21 | $7700.00 | Window Closed / Lapsed |