Patent US3315986A successfully refreshed from Google Patents.
US3003918A
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Production of ceruloplasmin in a purified state from blood plasma fractions
Assignee: Merck and Co Inc
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Filing: 1958-10-17
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Granted: 1961-10-10
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Expires: 1978-10-17
Abstract
No abstract record available.
Inventors
- 👤 Benjamin E Sanders
- 👤 Maurel N Richard
- 👤 Orin P Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:17
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/81; C07K14/8107; C07K14/811; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
151 words
IN A METHOD OF PRODUCING CERULOPLASMIN FROM A PROTEIN FRACTION SELECTED FROM THE GROUP CONSISTING OF COHN ALCOHOL FRACTIONS IV-1 AND IV-1,4, THE STPES WHICH COMPRISE STIRRING SAID FRACTION WITH AN ALKALI METAL CHLORIDE SOLUTION, CENTRIFUGING TO SEPARATE THE INSOLUBLE MATERIALS FROM THE RESULTING CERULOPLASMIN CONTAINING SUSPENSION, ADJUSTING THE PH OF THE RESULTING CERULOPLASMIN SUSPENSION TO ABOUT 4.6 TO 5, ADJUSTING THE SPECIFIC ELECTRICAL RESISTANCE OF THE SUSPENSION TO A VALUE ABOVE ABOUT 250 OHMS AT ABOUT 0 TO 4*C., SUSPENDING DIETHYLAMINOETHYLCELLULOSE IN A SOLUTION OF ALKALI METAL CHLORIDE AND ACETATE, SAID SOLUTION HAVING A PH OF ABOUT 4.6 TO 5 AND HAVING AN ALKALI METAL CHLORIDE MOLARITY OF AT LEAST ABOUT 0.025 M, MIXING THE SOLUTIONS TOGETHER CAUSING ADSORPTION OF SAID CERULOPLASMIN ON SAID DIETHYLAMINOETHYLCELLULOSE, FILTERING THE SOLUTION FROM THE RESULTING SOLIDS, AND ELUTING THE RESULTING CERULOPLASMIN FROM THE DIETHYLAMINOETHYLCELLULOSE BY INCREASING THE MOLAR CONCENTRATION OF SAID ALKALI METAL CHLORIDE.
Claim 2
Dependent Claim
151 words
IN A METHOD OF PRODUCING CERULOPLASMIN FROM A PROTEIN FRACTION SELECTED FROM THE GROUP CONSISTING OF COHN ALCOHOL FRACTIONS IV-1 AND IV-1,4, THE STPES WHICH COMPRISE STIRRING SAID FRACTION WITH AN ALKALI METAL CHLORIDE SOLUTION, CENTRIFUGING TO SEPARATE THE INSOLUBLE MATERIALS FROM THE RESULTING CERULOPLASMIN CONTAINING SUSPENSION, ADJUSTING THE PH OF THE RESULTING CERULOPLASMIN SUSPENSION TO ABOUT 4.6 TO 5, ADJUSTING THE SPECIFIC ELECTRICAL RESISTANCE OF THE SUSPENSION TO A VALUE ABOVE ABOUT 250 OHMS AT ABOUT 0 TO 4*C., SUSPENDING DIETHYLAMINOETHYLCELLULOSE IN A SOLUTION OF ALKALI METAL CHLORIDE AND ACETATE, SAID SOLUTION HAVING A PH OF ABOUT 4.6 TO 5 AND HAVING AN ALKALI METAL CHLORIDE MOLARITY OF AT LEAST ABOUT 0.025 M, MIXING THE SOLUTIONS TOGETHER CAUSING ADSORPTION OF SAID CERULOPLASMIN ON SAID DIETHYLAMINOETHYLCELLULOSE, FILTERING THE SOLUTION FROM THE RESULTING SOLIDS, AND ELUTING THE RESULTING CERULOPLASMIN FROM THE DIETHYLAMINOETHYLCELLULOSE BY INCREASING THE MOLAR CONCENTRATION OF SAID ALKALI METAL CHLORIDE.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1964-10-09 | 1965-04-10 | 1965-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1968-10-09 | 1969-04-10 | 1969-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1972-10-09 | 1973-04-10 | 1973-10-10 | $7700.00 | Window Closed / Lapsed |