Patent US3325787A successfully refreshed from Google Patents.
US2997425A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of purification of streptokinase
Assignee: Ortho Pharmaceutical Corp
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Filing: 1959-05-05
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Granted: 1961-08-22
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Expires: 1979-05-05
Abstract
No abstract record available.
Inventors
- 👤 Singher Heron Orlando
- 👤 Egan Richard
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:10
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/315; C07K14/3153; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
200 words
THE METHOD OF PURIFYING STREPTOKINASE WHICH COMPRISES DISSOLVING SUBSTANTIALLY SALT-FREE POWDERED STREPTOKINASE IN AN AQUEOUS SOLUTION BUFFERED AT A PH OF ABOUT 5.7 TO 7.7, ADDING THE SOLUTION TO AN ADSORBENT COLUMN OF DIETHYLAMINOETHYL CELLULOSE WHICH HAS BEEN PREPARED FOR PASSING THERETHROUGH AN AQUEOUS SOLUTION BUFFERED AT A PH OF ABOUT 5.7 TO 7.7, WHEREBY STREPTOKINASE IS ADSORBED ON THE ADSORBENT, ADDING TO THE COLUMN AQUEOUS SOLUTION BUFFERED AT A PH OF ABOUT 5.7 TO 7.7 WHEREBY INACTIVE PROTEINACEOUS MATERIAL IS REMOVED FROM THE ADSORBENT, ADDING TO THE COLUMN AQUEOUS BUFFER SOLUTION HAVING A PH OF ABOUT 5.7 TO 7.7 AND AN IONIC STRENGHT OF 0.1, WHEREBY ADDITIONAL INACTIVE PROTEINACEOUS MATERIAL IS REMOVED FROM THE ADSORBENT, ADDING TO THE COLUMN AN AMOUNT OF AQUEOUS BUFFER SOLUTION EQUAL TO AT LEAST ABOUT TWICE THE HOLDUP VOLUME OF THE COLUMN AND HAVING A PH OF ABOUT 5.7 TO 7.7 AND AN IONIC STRENGTH AT THE BEGINNING OF THE ADDITION OF 0.1 AND GRADUALLY INCREASING DURING THE ADDITION SO THAT AT THE END THEREOF THE IONIC STRENGTH IS 0.11 TO 0.13, WHEREBY STREPTOKINASE IS REMOVED FROM THE ADSORBENT AND A SOLUTION OF SUBSTANTIALLY PURE STREPTOKINASE IS OBTAINED, AND RECOVERING STREPTOKINASE FROM THE SOLUTION.
Claim 2
Independent Claim
200 words
THE METHOD OF PURIFYING STREPTOKINASE WHICH COMPRISES DISSOLVING SUBSTANTIALLY SALT-FREE POWDERED STREPTOKINASE IN AN AQUEOUS SOLUTION BUFFERED AT A PH OF ABOUT 5.7 TO 7.7, ADDING THE SOLUTION TO AN ADSORBENT COLUMN OF DIETHYLAMINOETHYL CELLULOSE WHICH HAS BEEN PREPARED FOR PASSING THERETHROUGH AN AQUEOUS SOLUTION BUFFERED AT A PH OF ABOUT 5.7 TO 7.7, WHEREBY STREPTOKINASE IS ADSORBED ON THE ADSORBENT, ADDING TO THE COLUMN AQUEOUS SOLUTION BUFFERED AT A PH OF ABOUT 5.7 TO 7.7 WHEREBY INACTIVE PROTEINACEOUS MATERIAL IS REMOVED FROM THE ADSORBENT, ADDING TO THE COLUMN AQUEOUS BUFFER SOLUTION HAVING A PH OF ABOUT 5.7 TO 7.7 AND AN IONIC STRENGHT OF 0.1, WHEREBY ADDITIONAL INACTIVE PROTEINACEOUS MATERIAL IS REMOVED FROM THE ADSORBENT, ADDING TO THE COLUMN AN AMOUNT OF AQUEOUS BUFFER SOLUTION EQUAL TO AT LEAST ABOUT TWICE THE HOLDUP VOLUME OF THE COLUMN AND HAVING A PH OF ABOUT 5.7 TO 7.7 AND AN IONIC STRENGTH AT THE BEGINNING OF THE ADDITION OF 0.1 AND GRADUALLY INCREASING DURING THE ADDITION SO THAT AT THE END THEREOF THE IONIC STRENGTH IS 0.11 TO 0.13, WHEREBY STREPTOKINASE IS REMOVED FROM THE ADSORBENT AND A SOLUTION OF SUBSTANTIALLY PURE STREPTOKINASE IS OBTAINED, AND RECOVERING STREPTOKINASE FROM THE SOLUTION.
References Cited (Backward Citations)
US2753291A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1964-08-21 | 1965-02-20 | 1965-08-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1968-08-21 | 1969-02-20 | 1969-08-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1972-08-21 | 1973-02-20 | 1973-08-22 | $7700.00 | Window Closed / Lapsed |