US2996429A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for growing living tissue
Assignee: NAT TOXICOLOGICAL LAB Inc
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Filing: 1959-02-12
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Granted: 1961-08-15
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Expires: 1979-02-12
Abstract
No abstract record available.
Inventors
- 👤 Jr Harry A Toulmin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 17:03
CPC Classifications
C; C12; C12M; C12M21/00; C12M21/02; C12M21/08; C12M27/00; C12M27/10; C12M29/00; C12M29/04; C12M31/00; C12M31/10
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
111 words
A METHOD OF GROWING ANIMAL TISSUE COMPRISING THE STEPS OF PROVIDING A CHAMBER ENCLOSING A NUTRIENT BATH OF ANIMAL SERUM AND EMBRYO EXTRACT, ARRANGING A DIALYZING MEMBRANE OVER THE SURFACE OF A DRUM WHICH IS DISPOSED IN THE CHAMBER AND PARTIALLY SUBMERGED IN SAID NUTRIENT BATH, ROTATING THE DRUM TO CAUSE SAID MEMBRANE TO MOVE IN AND OUT OF THE NUTRIENT BATH, MOUNTING A TISSUE EXPLANT ON ONE SIDE OF SAID MEMBRANE, CONDITIONING THE ATMOSPHERE IN THE CHAMBER BY INTRODUCING A GASEOUS MIXTURE OF OXYGEN, NITROGEN AND CARBON DIOXIDE, AND EXPOSING THE TISSUE EXPLANT ON SAID MEMBRANE TO LIGHT WAVES WHILE MOVING THE MEMBRANE IN AND OUT OF THE NUTRIENT BATH.
Claim 2
Independent Claim
111 words
A METHOD OF GROWING ANIMAL TISSUE COMPRISING THE STEPS OF PROVIDING A CHAMBER ENCLOSING A NUTRIENT BATH OF ANIMAL SERUM AND EMBRYO EXTRACT, ARRANGING A DIALYZING MEMBRANE OVER THE SURFACE OF A DRUM WHICH IS DISPOSED IN THE CHAMBER AND PARTIALLY SUBMERGED IN SAID NUTRIENT BATH, ROTATING THE DRUM TO CAUSE SAID MEMBRANE TO MOVE IN AND OUT OF THE NUTRIENT BATH, MOUNTING A TISSUE EXPLANT ON ONE SIDE OF SAID MEMBRANE, CONDITIONING THE ATMOSPHERE IN THE CHAMBER BY INTRODUCING A GASEOUS MIXTURE OF OXYGEN, NITROGEN AND CARBON DIOXIDE, AND EXPOSING THE TISSUE EXPLANT ON SAID MEMBRANE TO LIGHT WAVES WHILE MOVING THE MEMBRANE IN AND OUT OF THE NUTRIENT BATH.
Claim 3
Dependent Claim
120 words
IN AN APPARATUS FOR GROWING ANIMAL TISSUE, A CHAMBER, MEANS FOR INTRODUCING A LIQUID NUTRIENT INTO SAID CHAMBER, A ROTATABLE DRUM IN SAID CHAMBER, SAID DRUM BEING CLOSED AT ITS OPPOSITE ENDS AND HAVING A PLURALITY OF OPENINGS OVER THE SURFACE THEREOF, MEANS COMPRISING A DIALYZING MEMBRANE MOUNTED ON SAID DRUM AND COVERING SAID OPENINGS, SAID MEMBRANE BEING ADAPTED TO CARRY A TISSUE EXPLANT, MEANS FOR ROTATING SAID DRUM TO PERIODICALLY MOVE THE MEMBRANE THROUGH SAID NUTRIENT, MEANS FOR INTRODUCING A GASEOUS MIXTURE OF OXYGEN, NITROGEN AND CARBON DIOXIDE INTO SAID CHAMBER TO PROVIDE AN ATMOSPHERE FOR SAID TISSUE EXPLANT, AND MEANS COMPRISING A LIGHT SOURCE DISPOSED IN SAID CHAMBER FOR SUBJECTING SAID MEMBRANE TO THE ACTION OF LIGHT WAVES.
Claim 4
Dependent Claim
120 words
IN AN APPARATUS FOR GROWING ANIMAL TISSUE, A CHAMBER, MEANS FOR INTRODUCING A LIQUID NUTRIENT INTO SAID CHAMBER, A ROTATABLE DRUM IN SAID CHAMBER, SAID DRUM BEING CLOSED AT ITS OPPOSITE ENDS AND HAVING A PLURALITY OF OPENINGS OVER THE SURFACE THEREOF, MEANS COMPRISING A DIALYZING MEMBRANE MOUNTED ON SAID DRUM AND COVERING SAID OPENINGS, SAID MEMBRANE BEING ADAPTED TO CARRY A TISSUE EXPLANT, MEANS FOR ROTATING SAID DRUM TO PERIODICALLY MOVE THE MEMBRANE THROUGH SAID NUTRIENT, MEANS FOR INTRODUCING A GASEOUS MIXTURE OF OXYGEN, NITROGEN AND CARBON DIOXIDE INTO SAID CHAMBER TO PROVIDE AN ATMOSPHERE FOR SAID TISSUE EXPLANT, AND MEANS COMPRISING A LIGHT SOURCE DISPOSED IN SAID CHAMBER FOR SUBJECTING SAID MEMBRANE TO THE ACTION OF LIGHT WAVES.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1964-08-14 | 1965-02-13 | 1965-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1968-08-14 | 1969-02-13 | 1969-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1972-08-14 | 1973-02-13 | 1973-08-15 | $7700.00 | Window Closed / Lapsed |