Genetic transformation of eucaryota

Abstract

Abstract PURPOSE:To inject DNA securely into cell and to carry out the titled transformation, by suspending an eucaryote in a DNA-containing solution, pricking the cell nucleus with a spicule from the outside, pulling it out. CONSTITUTION:An eucaryote (any of yeast, a fungus such as a mold, animal and plant, and, when one such as plant has a cell wall, the cell wall may be removed and the cell may be processed into protoplast) is placed on the Petri dish 1, a DNA-containing solution having the desired genetic information is added to the dish, the Petri dish 1 is placed on the objective 2, the central part of the cell nucleus is pricked through with the hypodermic needle 3, and it is pulled out, so that DNA is introduced into the cell. DNA is incorporated into chromosome without being modified at all. Consequently, almost in all cases, genetic information except that of injected DNA is not admixtured, and screening of genetic variant is not required. Useful for breeding of animal or plant, improvement of kinds, remedy for animal, etc.

Inventors

• 👤 Mitsuru Furusawa
• 👤 古沢　満
• 👤 Fumiichiro Yamamoto
• 👤 山本　文一郎

Bibliographic Details