GB2188638A
Expired / Lapsed
GB
⏱️ 0.0 years remaining
Chimeric antibodies
Assignee: Individual
•
Filing: 1987-03-26
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Granted: —
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Expires: 2007-03-26
Abstract
Abstract An altered antibody is produced by replacing the complementarity determining regions (CDRs) of a variable region of an immunoglobulin (Ig) with the CDRs from an Ig of different specificity, using recombinant DNA techniques. The gene coding sequences for producing the altered antibody may be produced by site-directed mutagenesis using long oligonucleotides.
Inventors
- 👤 Gregory Paul Winter
Bibliographic Details
Application Number
—
Jurisdiction
GB
Data Source
google_patents
Last Synchronized
2026-10-23 05:22
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/46; C07K16/461; C07K16/464; C07K2317/00; C07K2317/20; C07K2317/24; C07K2319/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
36 words
An altered antibody in which at least parts of the complementarity determining regions (CDRs) in the light or heavy chain variable domains have been replaced by analogous parts of CDRs from an antibody of 45 differentspecificity.
Claim 2
Dependent Claim
36 words
An altered antibody in which at least parts of the complementarity determining regions (CDRs) in the light or heavy chain variable domains have been replaced by analogous parts of CDRs from an antibody of 45 differentspecificity.
Claim 3
Dependent Claim
14 words
The altered antibody of claim 1, in which the entire CDRs have been replaced.
Claim 4
Dependent Claim
27 words
The altered antibody of claim 1 or claim 2. in which the variable domains in both the heavy and light chains have been altered by CDR replacement.
Claim 5
Dependent Claim
29 words
The altered antibody of anyone of claims 1 to 3 in which the CDRs from a mouse antibody are grafted 50 onto the framework regions of a human antibody.
Claim 6
Dependent Claim
22 words
The altered antibody of any one of claims 1 to 4, which has the structure of a natural antibody ora fragment thereof.
Claim 7
Independent Claim
123 words
A method for producing an altered antibody comprising: a) preparing a first replicable expression vector including a suitable promoter operably linked to a DNA 55 sequence which encodes at least a variable domain of an lg heavy or light chain, the variable domain com prising framework regions from a first antibody and CDRs comprising at least parts of the CDRs from a second antibody of different specificity; b) if necessary, preparing a second replicable expression vector including a suitable promoter operably linked to a DNA sequence which encodes at leastthe variable domain of a complementary [g light or heavy 60 chain respectively; c) transforming a cell linewith thefirst or both prepared vectors; and d) culturing said transformed cell lineto produce said altered antibody.
Claim 8
Independent Claim
123 words
A method for producing an altered antibody comprising: a) preparing a first replicable expression vector including a suitable promoter operably linked to a DNA 55 sequence which encodes at least a variable domain of an lg heavy or light chain, the variable domain com prising framework regions from a first antibody and CDRs comprising at least parts of the CDRs from a second antibody of different specificity; b) if necessary, preparing a second replicable expression vector including a suitable promoter operably linked to a DNA sequence which encodes at leastthe variable domain of a complementary [g light or heavy 60 chain respectively; c) transforming a cell linewith thefirst or both prepared vectors; and d) culturing said transformed cell lineto produce said altered antibody.
Claim 9
Dependent Claim
31 words
The method of claim 6, in which the cell line which is transformed to produce the altered antibody is an immortalised cell line. 65 9 GB 2 188 638 A 9
Claim 10
Dependent Claim
20 words
The method of claim 7, in which the immortalised cell line is a myeloma cell line or a derivative thereof.
Claim 11
Independent Claim
23 words
The method of anyone of claims 6 to 8, in which the DNA sequence encoding the altered variable domain is prepared byoligonucleotide synthesis.
Claim 12
Independent Claim
63 words
The method of anyone of claims 6 to 8, in which the DNA sequence encoding the altered variable domain is prepared by primer directed oligonucleotide site-directed mutagenesis using along oli- 5 gonucleotide. Printed for Her Majesty's Stationery Office by Croydon Printing Company (11 K) Ltd,8187, D8991685. Published by The Patent Office, 25 Southampton Buildings, London WC2A l AY, from which copies maybe obtained.
References Cited (Backward Citations)
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