EP0509517A2
Expired / Lapsed
EP
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Oligosaccharide having affinity for fibroblast growth factor and process for producing same
Assignee: Seikagaku Corp
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Filing: 1992-04-16
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Granted: —
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Expires: 2012-04-16
Abstract
Abstract An oligosaccharide having an affinity for fibroblast growth factor, which is composed of 8 to 18 monosaccharide residues, wherein a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine and a process for producing the oligosaccharide comprising digesting heparan sulfate.
Inventors
- 👤 Hiroko Habuchi
- 👤 Sakaru Suzuki
- 👤 Koji Kimata
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 10:30
CPC Classifications
C; C07; C07H; C07H5/00; C07H5/08; C07H5/10; A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C08B37/0075; C08B37/0078; C12; C12P; C12P19/00; C12P19/26
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
28 words
An oligosaccharide having an affinity for fibroblast growth factor, which is composed of 8- to 18-monosaccharide residues with a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine.
Claim 2
Dependent Claim
39 words
A process for producing an oligosaccharide having an affinity for fibroblast growth factor and composed of 8- to 18-monosaccharide residues with a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine, which comprises digesting heparan sulfate with Heparitinase I.
Claim 3
Dependent Claim
36 words
A process of claim 2, wherein the resulting digest is incubated together with an acidic or basic fibroblast growth factor-bound carrier in the presence of chondroitin sulfate, and subsequently desorbing the bound digest from said carrier.
Claim 4
Dependent Claim
26 words
A process of claim 2, wherein the heparan sulfate is derived from small intestines or aortas of cattle, swine or sheep and cockscombs of domestic fowls.
Claim 5
Dependent Claim
11 words
A process of claim 3, wherein said carrier is agarose gel.
Claim 6
Dependent Claim
68 words
A process for producing an oligosaccharide having an affinity for fibroblast growth factor and composed of 8- to 18-monosaccharide residues with a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine, which comprises incubating heparan sulfate together with an acidic or basic fibroblast growth factor-bound carrier, isolating a fraction of the heparan sulfate which binds to said carrier and digesting the isolated heparan sulfate fraction with Heparitinase I.
Claim 7
Dependent Claim
36 words
A process of claim 6, wherein the resulting digest is incubated together with an acidic or basic fibroblast growth factor-bound carrier in the presence of chondroitin sulfate, and subsequently desorbing the bound digest from said carrier.
Claim 8
Dependent Claim
34 words
A composition comprising two or more of oligosaccharides having an affinity for fibroblast growth factor, which is composed of 8- to 18-monosaccharide residues with a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine.
Claim 9
Dependent Claim
44 words
A pharmaceutical composition comprising fibroblast growth factor and at least one of oligosaccharides having an affinity for fibroblast growth factor, which is composed of 8- to 18-monosaccharide residues with a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine, and a pharmaceutically acceptable carrier.
Claim 10
Dependent Claim
15 words
Use of an oligosaccharide according to claim 1 for the manufacture of a pharmaceutical composition.
References Cited (Backward Citations)
No citation records indexed.
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