EP0502496A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Novel heparitinase, process for producing the same and bacteria producing the same
Assignee: Seikagaku Corp
•
Filing: 1992-03-04
•
Granted: —
•
Expires: 2012-03-04
Abstract
Abstract Disclosed are novel enzymes, heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV, which degrade heparan sulfate and/or heparin, a process for producing thereof by cultivating a novel Bacillus circulans HpT 298 having an ability of producing these enzymes and a novel Bacillus circulans HpT 298.
Inventors
- 👤 Kiyoshi Morikawa
- 👤 Hirofumi Miyazono
- 👤 Hiroshi Maruyama
- 👤 Keiichi Yoshida
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 13:08
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/88; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/07; C12R2001/09; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/835
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
165 words
Novel enzymes, heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV, having the following properties: (A) action: each of enzymes is a lyase which acts on glucosaminide linkages of heparin or heparan sulfate, and cleaves them to form double bonds between 4-position and 5-position carbon atoms of uronic acids, (B) substrate specificity: heparitinase T-I and heparitinase T-II do not substantially act on heparin and they mainly act on haparan sulfate, and unsaturated disaccharides formed as degraded products are non-sulfate compound (ADiHS-OS) and a little amount of uronic acid-glucosamine-N-sulfate (ADiHS-NS); heparitinase T-III does not substantially act on heparin and it mainly acts on heparan sulfate, and unsaturated disaccharides formed as degraded product are non-sulfate compound (ADiHS-OS) and uronic acid-glucosamine-N-sulfate (ADiHS-NS); heparitinase T-IV acts on heparin and heparan sulfate, and unsaturated disaccharides formed as degraded product are uronic acid-glucosamine-N-sulfate (ADiHS-OS), uronic acid-glucosamine-N,6-disulfate (ADiHS-diN,6S), uronic acid-2-sulfate- glucosamine-N-sulfate (ΔDiHS-diU,NS) and uronic acid-2-sulfate-glucosamine-N,6-disulate (ADiHS-TriS), (C) optimum pH: (D) pH stability: (E) optimum temperature: (F) heat stability: (G) inhibition and activation:
Claim 2
Dependent Claim
165 words
Novel enzymes, heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV, having the following properties: (A) action: each of enzymes is a lyase which acts on glucosaminide linkages of heparin or heparan sulfate, and cleaves them to form double bonds between 4-position and 5-position carbon atoms of uronic acids, (B) substrate specificity: heparitinase T-I and heparitinase T-II do not substantially act on heparin and they mainly act on haparan sulfate, and unsaturated disaccharides formed as degraded products are non-sulfate compound (ADiHS-OS) and a little amount of uronic acid-glucosamine-N-sulfate (ADiHS-NS); heparitinase T-III does not substantially act on heparin and it mainly acts on heparan sulfate, and unsaturated disaccharides formed as degraded product are non-sulfate compound (ADiHS-OS) and uronic acid-glucosamine-N-sulfate (ADiHS-NS); heparitinase T-IV acts on heparin and heparan sulfate, and unsaturated disaccharides formed as degraded product are uronic acid-glucosamine-N-sulfate (ADiHS-OS), uronic acid-glucosamine-N,6-disulfate (ADiHS-diN,6S), uronic acid-2-sulfate- glucosamine-N-sulfate (ΔDiHS-diU,NS) and uronic acid-2-sulfate-glucosamine-N,6-disulate (ADiHS-TriS), (C) optimum pH: (D) pH stability: (E) optimum temperature: (F) heat stability: (G) inhibition and activation:
Claim 3
Dependent Claim
58 words
A process for producing heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV according to Claim 1, which comprises cultivating bacteria belonging to Bacillus and having an ability of producing heparitinase T-I, heparitinase T-II, heparitinase T-III and/or heparitinase T-IV and collecting heparitinase T-I, heparitinase T-II, heparitinase T-III and/or heparitinase T-IV from its culture broth or bacterial cells extract.
Claim 4
Dependent Claim
28 words
A Bacillus circulans HpT 298 having an ability of producing at least one of heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV as claimed in Claim 1.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.