EP0476556A2
Expired / Lapsed
EP
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Method for determination of a light sensitive substance
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1991-09-14
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Granted: —
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Expires: 2011-09-14
Abstract
Abstract There is disclosed a method for determination of a light sensitive substance wherein irradiation of lumigenic substance-light sensitive substance solution with modulated light is used to generate short wavelength light proportionally to the concentration of the light sensitive substance. Synchronous detection allows high sensitivity. The method provides for diagnostic assays of greater simplicity and convenience.
Inventors
- 👤 Marvin A. Motsenbocker
- 👤 Motoaki Shintani
- 👤 Koichi Kondo
- 👤 Hirotomo Masuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 19:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N21/00; G01N21/75; G01N21/76; G01N33/58; G01N33/582; G01N21/17; G01N2021/1789; G01N2021/1791
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
41 words
A method for determination of a light sensitive substance comprising irradiating the light sensitive substance in the presence of a lumigenic substance with modulated light and synchronously detecting shorter wavelength emitted light proportional to the concentration of the light sensitive substance.
Claim 2
Independent Claim
41 words
A method for determination of a light sensitive substance comprising irradiating the light sensitive substance in the presence of a lumigenic substance with modulated light and synchronously detecting shorter wavelength emitted light proportional to the concentration of the light sensitive substance.
Claim 3
Dependent Claim
26 words
A method according to claim 1, wherein the light sensitive substance is a member selected from the group consisting of xanthines, eosins, porphyrins, chlorins and thionines.
Claim 4
Dependent Claim
23 words
A method according to claim 1, wherein the lumigenic substance is a member selected from the group consisting of luminols, lucigenins and luciferins.
Claim 5
Dependent Claim
19 words
A method according to claim 1, wherein a laser or light emitting diode is used as the light source.
Claim 6
Dependent Claim
14 words
A method according to claim 1, wherein a lock-in amplifier is used for detection.
Claim 7
Dependent Claim
23 words
A method according to claim 1, wherein the light sensitive substance is conjugated to an antibody and participates in an immunoassay detection system.
Claim 8
Independent Claim
45 words
A method for determination of a light sensitive substance comprising pulse-irradiating the light sensitive substance in the presence of a lumigenic substance and detecting shorter wavelength emitted light in the absence of the pulsed excitation light proportional to the concentration of the light sensitive substance.
Claim 9
Independent Claim
45 words
A method for determination of a light sensitive substance comprising pulse-irradiating the light sensitive substance in the presence of a lumigenic substance and detecting shorter wavelength emitted light in the absence of the pulsed excitation light proportional to the concentration of the light sensitive substance.
Claim 10
Dependent Claim
26 words
A method according to claim 7, wherein the light sensitive substance is a member selected from the group consisting of xanthines, eosins, porphyrins, chlorins and thionines.
Claim 11
Dependent Claim
23 words
A method according to claim 7, wherein the lumigenic substance is a member selected from the group consisting of luminols, lucigenins and luciferins.
Claim 12
Dependent Claim
19 words
A method according to claim 7, wherein a laser or light emitting diode is used as the light source.
Claim 13
Dependent Claim
23 words
A method according to claim 7, wherein the light sensitive substance is conjugated to an antibody and participates in an immunoassay detection system.
References Cited (Backward Citations)
No citation records indexed.
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