EP0459577A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Microbially expressed portions of a monoclonal antibody block rhinovirus attachment to cell receptors
Assignee: Merck and Co Inc
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Filing: 1991-05-25
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Granted: —
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Expires: 2011-05-25
Abstract
Abstract Complementary DNA (cDNA) fragments encoding the Fab and Fv portion of the light and heavy chains of a murine monoclonal antibody (mAb) were prepared from hybridoma messenger RNA (mRNA). The mAb is specific for the intercellular adhesion molecule-1 (ICAM-1). Furthermore, the mAb blocks the attachment of human rhinovirus (HRV) particles to their cellular receptor, ICAM-1. Fab and Fv cDNA were molecularly cloned and expressed in a recombinant host, purified, and shown to be biologically active in that they bind to ICAM-1, and block attachment of ligands to ICAM-1, including HRV.
Inventors
- 👤 Richard J. Colonno
- 👤 Jon H. Condra
- 👤 Joanne E. Tomassini
- 👤 Vinod V. Sardana
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 12:49
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2803; C07K16/2821; A; A61; A61P; A61P29/00; A61K; A61K38/00
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
8 words
A peptide selected form the group consisting of:
Claim 2
Dependent Claim
15 words
A composition of peptides consisting essentially of peptide (A) and peptide (B) of Claim 1.
Claim 3
Dependent Claim
15 words
A composition of peptides consisting essentially of peptide (C) and peptide (D) of Claim 1.
Claim 4
Dependent Claim
15 words
A composition of peptides consisting essentially of peptide (E) and peptide (F) of Claim 1.
Claim 5
Dependent Claim
8 words
A peptide which has the amino acid sequence:
Claim 6
Dependent Claim
16 words
A peptide according to Claim 5 which corresponds to the Fv portion of an immunoglobulin molecule.
Claim 7
Dependent Claim
29 words
A peptide or composition of peptides which corresponds to the Fab or Fv portion of an immunoglobulin molecule which is specific for the cellular receptor of a respiratory virus.
Claim 8
Dependent Claim
20 words
The peptide or composition of peptides according to Claim 7, which is specific for the cellular receptor of a rhinovirus.
Claim 9
Dependent Claim
28 words
A peptide or composition of peptides according to Claim 8, which is capable of blocking the attachment of serotypes of the majority of rhinoviruses to a susceptible cell.
Claim 10
Dependent Claim
26 words
A peptide or composition of peptides according to Claim 8, which is capable of displacing rhinovirus virions when those virions are bound to a susceptible cell.
Claim 11
Dependent Claim
20 words
A pharmaceutical composition comprising a peptide or peptides according to Claim 7 for the prevention and treatment of rhinovirus infection.
Claim 12
Dependent Claim
22 words
A formulation for intranasal instillation of a peptide or peptides according to Claim 7 for the prevention and treatment of rhinovirus infection.
Claim 13
Dependent Claim
27 words
A peptide or composition of peptides which corresponds to the Fab or Fv portion of an immunoglobulin molecule which can bind to the intercellular adhesion molecule-1, ICAM-1.
Claim 14
Dependent Claim
14 words
A peptide according to Claim 13 which prevents a ligand from binding to ICAM-1.
Claim 15
Dependent Claim
13 words
A peptide according to Claim 14 which binds to domain I of ICAM-1.
Claim 16
Dependent Claim
12 words
A pharmaceutical composition containing a peptide or peptides according to Claim 14.
Claim 17
Dependent Claim
20 words
A peptide or composition of peptides according to Claim 14 which can bind to and inactivate the intercellular adhesion molecule-1.
Claim 18
Dependent Claim
12 words
A pharmaceutical composition containing a peptide or peptides according to Claim 17.
References Cited (Backward Citations)
No citation records indexed.
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